1887

Abstract

Two Gram-negative, rod-shaped bacteria, strains E96 and E90, were isolated from medicinal leeches () and characterized by a polyphasic taxonomic approach. Phylogenetic analysis based on the nearly full-length 16S rRNA gene sequence showed that the two strains shared 98.1 % sequence similarity and were affiliated with the genus within the phylum , with 94.4–97.6 % sequence similarity to type strains of species of the genus and highest sequence similarity to the type strain of (97.3 and 97.6 %, respectively). -related 16S rRNA gene sequences were recently detected in the bladders of ; however, no cultured representatives were so far available. Genomic fingerprint analysis using repetitive element primed (rep)- and randomly amplified polymorphic DNA (RAPD)-PCRs and DNA–DNA hybridization experiments clearly showed that the strains were different from each other (DNA–DNA relatedness values of 39.1 %, reciprocal 28.0 %) and from the type strains of (<19.7 %) and (<41.1 %). Chemotaxonomic analyses confirmed the affiliation to the genus . Both strains contained MK-7 as the predominant menaquinone. The major fatty acids of both strains were iso-C, iso-C G, iso-C 3-OH and summed feature 3 (Cω7 and/or iso-C 2-OH), which is characteristic for the genus . Based on genotypic, chemotaxonomic and physiological characterization, we propose two novel species of the genus , sp. nov., with strain E96 ( = DSM 25812 = CCM 8411 = LMG 26956) as the type strain, and sp. nov., with strain E90 ( = DSM 25811 = CCM 8410 = LMG 26954) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.050823-0
2013-09-01
2020-05-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/9/3487.html?itemId=/content/journal/ijsem/10.1099/ijs.0.050823-0&mimeType=html&fmt=ahah

References

  1. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. ( 1978 ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . . Proc Natl Acad Sci U S A 75, 48014805. [CrossRef] [PubMed]
    [Google Scholar]
  2. Collins M. D., Jones D. ( 1980 ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48, 459470. [CrossRef]
    [Google Scholar]
  3. Collins M. D., Goodfellow M., Minnikin D. E. ( 1979 ). Isoprenoid quinones in the classification of coryneform and related bacteria. . J Gen Microbiol 110, 127136. [CrossRef] [PubMed]
    [Google Scholar]
  4. Dai J., Jiang F., Wang Y., Yu B., Qi H., Fang C., Zheng C. ( 2011 ). Niabella tibetensis sp. nov., isolated from soil, and emended description of the genus Niabella . . Int J Syst Evol Microbiol 61, 12011205. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1985 ). Confidence limits of phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  6. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  7. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. ( 1996 ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46, 234239. [CrossRef] [PubMed]
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;.
    [Google Scholar]
  9. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [CrossRef]
    [Google Scholar]
  10. Kämpfer P., Steiof M., Dott W. ( 1991 ). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. . Microb Ecol 21, 227251. [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Lodders N., Martin K., Avendaño-Herrera R. ( 2012 ). Flavobacterium chilense sp. nov. and Flavobacterium araucananum sp. nov., isolated from farmed salmonid fish. . Int J Syst Evol Microbiol 62, 14021408. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kikuchi Y., Bomar L., Graf J. ( 2009 ). Stratified bacterial community in the bladder of the medicinal leech, Hirudo verbana . . Environ Microbiol 11, 27582770. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kim B. Y., Weon H. Y., Yoo S. H., Hong S. B., Kwon S. W., Stackebrandt E., Go S. J. ( 2007 ). Niabella aurantiaca gen. nov., sp. nov., isolated from a greenhouse soil in Korea. . Int J Syst Evol Microbiol 57, 538541. [CrossRef] [PubMed]
    [Google Scholar]
  14. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  15. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [CrossRef] [PubMed]
    [Google Scholar]
  16. Minnikin D. E., Collins M. D., Goodfellow M. ( 1979 ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47, 8795. [CrossRef]
    [Google Scholar]
  17. Pitcher D. G., Saunders N. A., Owen R. J. ( 1989 ). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. . Lett Appl Microbiol 8, 151156. [CrossRef]
    [Google Scholar]
  18. Stamatakis A. ( 2006 ). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22, 26882690. [CrossRef] [PubMed]
    [Google Scholar]
  19. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  20. Versalovic J., Koeuth T., Lupski J. R. ( 1991 ). Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. . Nucleic Acids Res 19, 68236831. [CrossRef] [PubMed]
    [Google Scholar]
  21. Versalovic J., Schneider M., de Bruijn F. J., Lupski J. R. ( 1994 ). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. . Methods Mol Cell Biol 5, 2540.
    [Google Scholar]
  22. Wang H., Zhang Y. Z., Man C. X., Chen W. F., Sui X. H., Li Y., Zhang X. X., Chen W. X. ( 2009 ). Niabella yanshanensis sp. nov., isolated from the soybean rhizosphere. . Int J Syst Evol Microbiol 59, 28542856. [CrossRef] [PubMed]
    [Google Scholar]
  23. Weon H. Y., Kim B. Y., Joa J. H., Kwon S. W., Kim W. G., Koo B. S. ( 2008 ). Niabella soli sp. nov., isolated from soil from Jeju Island, Korea. . Int J Syst Evol Microbiol 58, 467469. [CrossRef] [PubMed]
    [Google Scholar]
  24. Weon H. Y., Yoo S. H., Kim B. Y., Son J. A., Kim Y. J., Kwon S. W. ( 2009 ). Niabella ginsengisoli sp. nov., isolated from soil cultivated with Korean ginseng. . Int J Syst Evol Microbiol 59, 12821285. [CrossRef] [PubMed]
    [Google Scholar]
  25. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R. ( 2008 ). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31, 241250. [CrossRef] [PubMed]
    [Google Scholar]
  26. Ziemke F., Brettar I., Höfle M. G. ( 1997 ). Stability and diversity of the genetic structure of a Shewanella putrefaciens population in the water column of the central Baltic. . Aquat Microb Ecol 13, 6374. [CrossRef]
    [Google Scholar]
  27. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. ( 1998 ). Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov.. Int J Syst Bacteriol 48, 179186. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.050823-0
Loading
/content/journal/ijsem/10.1099/ijs.0.050823-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error