1887

Abstract

Two Gram-negative, rod-shaped bacteria, strains E96 and E90, were isolated from medicinal leeches () and characterized by a polyphasic taxonomic approach. Phylogenetic analysis based on the nearly full-length 16S rRNA gene sequence showed that the two strains shared 98.1 % sequence similarity and were affiliated with the genus within the phylum , with 94.4–97.6 % sequence similarity to type strains of species of the genus and highest sequence similarity to the type strain of (97.3 and 97.6 %, respectively). -related 16S rRNA gene sequences were recently detected in the bladders of ; however, no cultured representatives were so far available. Genomic fingerprint analysis using repetitive element primed (rep)- and randomly amplified polymorphic DNA (RAPD)-PCRs and DNA–DNA hybridization experiments clearly showed that the strains were different from each other (DNA–DNA relatedness values of 39.1 %, reciprocal 28.0 %) and from the type strains of (<19.7 %) and (<41.1 %). Chemotaxonomic analyses confirmed the affiliation to the genus . Both strains contained MK-7 as the predominant menaquinone. The major fatty acids of both strains were iso-C, iso-C G, iso-C 3-OH and summed feature 3 (Cω7 and/or iso-C 2-OH), which is characteristic for the genus . Based on genotypic, chemotaxonomic and physiological characterization, we propose two novel species of the genus , sp. nov., with strain E96 ( = DSM 25812 = CCM 8411 = LMG 26956) as the type strain, and sp. nov., with strain E90 ( = DSM 25811 = CCM 8410 = LMG 26954) as the type strain.

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2013-09-01
2019-10-14
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