1887

Abstract

A novel Gram-staining-negative, moderately halophilic and strictly aerobic bacterium, designated strain 112, was isolated from a tidal flat at Taean, Korea. Cells were catalase- and oxidase-positive ovoids to rods and did not produce bacteriochlorophyll . Optimum growth of strain 112 was observed at 30 °C, at pH 6.5–7.5 and in the presence of 2–4 % (w/v) NaCl. The major cellular fatty acids were summed feature 8 (Cω7 and/or Cω6) and C and Q-10 was detected as the predominant ubiquinone. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid and three unidentified lipids. The genomic DNA G+C content of strain 112 was 58.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 112 formed a phyletic lineage within the genus of the class . Strain 112 was most closely related to DFL-24, with 96.52 % similarity. On the basis of phenotypic, chemotaxonomic and molecular properties, strain 112 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 112 ( = KACC 16185 = JCM 17743).

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2013-10-01
2019-10-17
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References

  1. Allgaier M., Uphoff H., Felske A., Wagner-Döbler I.. ( 2003;). Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats. . Appl Environ Microbiol 69:, 5051–5059. [CrossRef][PubMed]
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  3. Biebl H., Allgaier M., Lünsdorf H., Pukall R., Tindall B. J., Wagner-Döbler I.. ( 2005;). Roseovarius mucosus sp. nov., a member of the Roseobacter clade with trace amounts of bacteriochlorophyll a. . Int J Syst Evol Microbiol 55:, 2377–2383. [CrossRef][PubMed]
    [Google Scholar]
  4. Boettcher K. J., Geaghan K. K., Maloy A. P., Barber B. J.. ( 2005;). Roseovarius crassostreae sp. nov., a member of the Roseobacter clade and the apparent cause of juvenile oyster disease (JOD) in cultured Eastern oysters. . Int J Syst Evol Microbiol 55:, 1531–1537. [CrossRef][PubMed]
    [Google Scholar]
  5. DeSantis T. Z. Jr, Hugenholtz P., Keller K., Brodie E. L., Larsen N., Piceno Y. M., Phan R., Andersen G. L.. ( 2006;). nast: a multiple sequence alignment server for comparative analysis of 16S rRNA genes. . Nucleic Acids Res 34:, W394–W399. [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. ( 2002;). phylip (phylogeny inference package), version 3.6a. . Distributed by the author. Department of Genome Sciences, University of Washington;, Seattle, USA:.
  7. Garrity G. M., Bell J. A., Lilburn T.. ( 2005;). Family I. Rhodobacteraceae fam. nov.. In Bergey's Manual of Systematic Bacteriology, , 2nd edn., vol. 2C, pp. 161–229. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M... New York:: Springer;.
    [Google Scholar]
  8. Gomori G.. ( 1955;). Preparation of buffers for use in enzyme studies. . Methods Enzymol 1:, 138–146. [CrossRef]
    [Google Scholar]
  9. Gonzalez J. M., Saiz-Jimenez C.. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4:, 770–773. [CrossRef][PubMed]
    [Google Scholar]
  10. González J. M., Covert J. S., Whitman W. B., Henriksen J. R., Mayer F., Scharf B., Schmitt R., Buchan A., Fuhrman J. A.. & other authors ( 2003;). Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments. . Int J Syst Evol Microbiol 53:, 1261–1269. [CrossRef][PubMed]
    [Google Scholar]
  11. Jeon C. O., Park W., Ghiorse W. C., Madsen E. L.. ( 2004;). Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. . Int J Syst Evol Microbiol 54:, 93–97. [CrossRef][PubMed]
    [Google Scholar]
  12. Jeong S. H., Park M. S., Jin H. M., Lee K., Park W., Jeon C. O.. ( 2013;). Aestuariibaculum suncheonense gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a tidal flat and emended descriptions of the genera Gaetbulibacter and Tamlana. . Int J Syst Evol Microbiol 63:, 332–338. [CrossRef][PubMed]
    [Google Scholar]
  13. Jin H. M., Lee H. J., Kim J. M., Park M. S., Lee K., Jeon C. O.. ( 2011;). Litorimicrobium taeanense gen. nov., sp. nov., isolated from a sandy beach. . Int J Syst Evol Microbiol 61:, 1392–1396. [CrossRef][PubMed]
    [Google Scholar]
  14. Jin H. M., Kim J. M., Lee H. J., Madsen E. L., Jeon C. O.. ( 2012;). Alteromonas as a key agent of polycyclic aromatic hydrocarbon biodegradation in crude oil-contaminated coastal sediment. . Environ Sci Technol 46:, 7731–7740. [CrossRef][PubMed]
    [Google Scholar]
  15. Jung Y. T., Lee J. S., Oh K. H., Oh T. K., Yoon J. H.. ( 2011;). Roseovarius marinus sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 61:, 427–432. [CrossRef][PubMed]
    [Google Scholar]
  16. Jung Y. T., Park S., Yoon J. H.. ( 2012;). Roseovarius litoreus sp. nov., isolated from seawater of southern coast of Korean peninsula. . Antonie van Leeuwenhoek 102:, 141–148. [CrossRef][PubMed]
    [Google Scholar]
  17. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012a;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  18. Kim Y. O., Kong H. J., Park S., Kang S. J., Kim W. J., Kim K. K., Oh T. K., Yoon J. H.. ( 2012b;). Roseovarius halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi. . Int J Syst Evol Microbiol 62:, 931–936. [CrossRef][PubMed]
    [Google Scholar]
  19. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  20. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Schumann P., Hirsch P.. ( 1999;). Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. . Int J Syst Bacteriol 49:, 137–147. [CrossRef][PubMed]
    [Google Scholar]
  21. Lai Q., Zhong H., Wang J., Yuan J., Sun F., Wang L., Zheng T., Shao Z.. ( 2011;). Roseovarius indicus sp. nov., isolated from deep-sea water of the Indian Ocean. . Int J Syst Evol Microbiol 61:, 2040–2044. [CrossRef][PubMed]
    [Google Scholar]
  22. Lányi B.. ( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  23. Leifson E.. ( 1963;). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  24. Martens T., Heidorn T., Pukall R., Simon M., Tindall B. J., Brinkhoff T.. ( 2006;). Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera. . Int J Syst Evol Microbiol 56:, 1293–1304. [CrossRef][PubMed]
    [Google Scholar]
  25. Math R. K., Jin H. M., Kim J. M., Hahn Y., Park W., Madsen E. L., Jeon C. O.. ( 2012;). Comparative genomics reveals adaptation by Alteromonas sp. SN2 to marine tidal-flat conditions: cold tolerance and aromatic hydrocarbon metabolism. . PLoS ONE 7:, e35784. [CrossRef][PubMed]
    [Google Scholar]
  26. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  27. Oh Y. S., Lim H. J., Cha I. T., Im W. T., Yoo J. S., Kang U. G., Rhee S. K., Roh D. H.. ( 2009;). Roseovarius halotolerans sp. nov., isolated from deep seawater. . Int J Syst Evol Microbiol 59:, 2718–2723. [CrossRef][PubMed]
    [Google Scholar]
  28. Park S., Yoon J. H.. ( 2013;). Roseovarius sediminilitoris sp. nov., isolated from seashore sediment. . Int J Syst Evol Microbiol 63:, 1741–1745. [CrossRef][PubMed]
    [Google Scholar]
  29. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. , MIDI Technical Note 101. Newark, DE:: MIDI Inc.;
  30. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  31. Stamatakis A., Ott M., Ludwig T.. ( 2005;). RAxML-OMP: an efficient program for phylogenetic inference on SMPs. . In Parallel Computing Technologies (Lecture Notes in Computer Science, vol. 3606), pp. 288–302. Edited by Malyshkin V... Berlin, Heidelberg:: Springer;. [CrossRef]
    [Google Scholar]
  32. Wang Q., Garrity G. M., Tiedje J. M., Cole J. R.. ( 2007;). Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. . Appl Environ Microbiol 73:, 5261–5267. [CrossRef][PubMed]
    [Google Scholar]
  33. Wang B., Tan T., Shao Z.. ( 2009;). Roseovarius pacificus sp. nov., isolated from deep-sea sediment. . Int J Syst Evol Microbiol 59:, 1116–1121. [CrossRef][PubMed]
    [Google Scholar]
  34. Wang B., Sun F., Lai Q., Du Y., Liu X., Li G., Luo J., Shao Z.. ( 2010;). Roseovarius nanhaiticus sp. nov., a member of the Roseobacter clade isolated from marine sediment. . Int J Syst Evol Microbiol 60:, 1289–1295. [CrossRef][PubMed]
    [Google Scholar]
  35. Winberg P. C., Lynch T. P., Murray A., Jones A. R., Davis A. R.. ( 2007;). The importance of spatial scale for the conservation of tidal flat macrobenthos: an example from New South Wales, Australia. . Biol Conserv 134:, 310–320. [CrossRef]
    [Google Scholar]
  36. Yoon J. H., Kang S. J., Oh T. K.. ( 2008;). Roseovarius aestuarii sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 58:, 1198–1202. [CrossRef][PubMed]
    [Google Scholar]
  37. Yurkov V., Stackebrandt E., Holmes A., Fuerst J. A., Hugenholtz P., Golecki J., Gad'on N., Gorlenko V. M., Kompantseva E. I., Drews G.. ( 1994;). Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov.. Int J Syst Bacteriol 44:, 427–434. [CrossRef][PubMed]
    [Google Scholar]
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