1887

Abstract

A novel Gram-stain-positive, non-endospore-forming, coccoid actinobacterium, designated strain D3-40, was isolated from the soil of a natural cave and characterized by means of a polyphasic taxonomic analysis. 16S rRNA gene sequence analysis showed that strain D3-40 is a member of the suborder and forms a distinct branch at the base of a cluster. Its closest relative is the type strain of (95.7 % sequence similarity). The chemotaxonomic characteristics were as follows: the cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid; the major menaquinone was MK-9(H); the polar lipids consisted of phosphatidylglycerol, phosphatidylinositol, an unknown glycolipid and an unknown phospholipid; the major fatty acids were anteiso-C, anteiso-C, iso-C, C and cyclohexyl-C; mycolic acids were absent. The G+C content of the DNA was 64.3 mol%. On the basis of morphological, chemotaxonomic and phylogenetic data, it is suggested that the organism represents a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is D3-40 ( = KCTC 29141 = DSM 26341).

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2013-11-01
2019-10-21
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