1887

Abstract

A novel Gram-stain-positive, non-endospore-forming, coccoid actinobacterium, designated strain D3-40, was isolated from the soil of a natural cave and characterized by means of a polyphasic taxonomic analysis. 16S rRNA gene sequence analysis showed that strain D3-40 is a member of the suborder and forms a distinct branch at the base of a cluster. Its closest relative is the type strain of (95.7 % sequence similarity). The chemotaxonomic characteristics were as follows: the cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid; the major menaquinone was MK-9(H); the polar lipids consisted of phosphatidylglycerol, phosphatidylinositol, an unknown glycolipid and an unknown phospholipid; the major fatty acids were anteiso-C, anteiso-C, iso-C, C and cyclohexyl-C; mycolic acids were absent. The G+C content of the DNA was 64.3 mol%. On the basis of morphological, chemotaxonomic and phylogenetic data, it is suggested that the organism represents a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is D3-40 ( = KCTC 29141 = DSM 26341).

Funding
This study was supported by the:
  • Jeju National University
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.050732-0
2013-11-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/11/3958.html?itemId=/content/journal/ijsem/10.1099/ijs.0.050732-0&mimeType=html&fmt=ahah

References

  1. Breed R. S. ( 1953 ). The families developed from Bacteriaceae Cohn with a description of the family Brevibacteriaceae Breed, 1953. . VI Congr Int Microbiol Roma 1, 10–15.
    [Google Scholar]
  2. Busse H.-J. ( 2012 ). Order Micrococcales . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 5, pp. 569570. Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K., Ludwig W., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  3. Collins M. D., Jones D., Keddie R. M., Sneath P. H. A. ( 1980 ). Reclassification of Chromobacterium iodinum (Davis) in a redefined genus Brevibacterium (Breed) as Brevibacterium iodinum nom. rev., comb. nov.. J Gen Microbiol 120, 110.
    [Google Scholar]
  4. Cui Y., Kang M.-S., Woo S.-G., Jin L., Kim K. K., Park J., Lee M., Lee S.-T. ( 2013 ). Brevibacterium daeguense sp. nov. a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture. . 63, 152157.
    [Google Scholar]
  5. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  6. Fitch W. M. ( 1971 ). Towards defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  7. Goodfellow M., Trujillo M. E. ( 2012 ). Genus Brevibacterium . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 5, pp. 685700. Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K., Ludwig W., Whitman W. B. . New York:: Springer;. [View Article]
    [Google Scholar]
  8. Heyrman J., Verbeeren J., Schumann P., Devos J., Swings J., De Vos P. ( 2004 ). Brevibacterium picturae sp. nov., isolated from a damaged mural painting at the Saint-Catherine chapel (Castle Herberstein, Austria). . Int J Syst Evol Microbiol 54, 15371541. [View Article] [PubMed]
    [Google Scholar]
  9. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. (editors) ( 1985 ). Genetic Manipulation of Streptomyces. A Laboratory Manual. Norwich:: The John Innes Foundation;.
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;.
    [Google Scholar]
  11. Kämpfer P., Schäfer J., Lodders N., Busse H. J. ( 2010 ). Brevibacterium sandarakinum sp. nov., isolated from a wall of an indoor environment. . Int J Syst Evol Microbiol 60, 909913. [View Article] [PubMed]
    [Google Scholar]
  12. Kim J., Srinivasan S., You T., Bang J. J., Park S., Lee S. S. ( 2013 ). Brevibacterium ammoniilyticum sp. nov., an ammonia-degrading bacterium isolated from sludge of a wastewater treatment plant. . Int J Syst Evol Microbiol 63, 11111118. [View Article] [PubMed]
    [Google Scholar]
  13. Kroppenstedt R. M. ( 1985 ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics, pp. 173199. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  14. Kumar A., İnce İ. A., Katı A., Chakraborty R. ( 2013 ). Brevibacterium siliguriense sp. nov., a facultatively oligotrophic bacterium isolated from river water. . Int J Syst Evol Microbiol 63, 511515. [View Article] [PubMed]
    [Google Scholar]
  15. Lee S. D. ( 2006 ). Brevibacterium samyangense sp. nov., an actinomycete isolated from a beach sediment. . Int J Syst Evol Microbiol 56, 18891892. [View Article] [PubMed]
    [Google Scholar]
  16. Manaia C. M., Moore E. R. B. ( 2002 ). Pseudomonas thermotolerans sp. nov., a thermotolerant species of the genus Pseudomonas sensu stricto . . Int J Syst Evol Microbiol 52, 22032209. [View Article] [PubMed]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  18. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104117. [View Article]
    [Google Scholar]
  19. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. ( 1980 ). Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188, 221233. [View Article]
    [Google Scholar]
  20. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  21. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313340. [View Article]
    [Google Scholar]
  22. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. ( 1997 ). Proposal for a new hierarchic classification system, Actinobacteria classis. nov.. Int J Syst Bacteriol 47, 479491. [View Article]
    [Google Scholar]
  23. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226231.[PubMed]
    [Google Scholar]
  24. Tang S.-K., Wang Y., Schumann P., Stackebrandt E., Lou K., Jiang C.-L., Xu L.-H., Li W.-J. ( 2008 ). Brevibacterium album sp. nov., a novel actinobacterium isolated from a saline soil in China. . Int J Syst Evol Microbiol 58, 574577. [View Article] [PubMed]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  26. Tonouchi A., Kitamura K., Fujita T. ( 2013 ). Brevibacterium yomogidense sp. nov., isolated from a soil conditioner made from poultry manure. . Int J Syst Evol Microbiol 63, 516520. [View Article] [PubMed]
    [Google Scholar]
  27. Vaz-Moreira I., Nobre M. F., Ferreira A. C. S., Schumann P., Nunes O. C., Manaia C. M. ( 2008 ). Humibacter albus gen. nov., sp. nov., isolated from sewage sludge compost. . Int J Syst Evol Microbiol 58, 10141018. [View Article] [PubMed]
    [Google Scholar]
  28. Zhi X.-Y., Li W.-J., Stackebrandt E. ( 2009 ). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. . Int J Syst Evol Microbiol 59, 589608. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.050732-0
Loading
/content/journal/ijsem/10.1099/ijs.0.050732-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error