1887

Abstract

A pink-pigmented, chemo-organotrophic bacterium, designated strain 03SUJ4, was isolated from the freshwater of Juam reservoir, Republic of Korea (35° 03′ 43′′ N 127° 14′ 15′′ E). Cells were aerobic, Gram-reaction-negative and non-motile rods. Strain 03SUJ4 grew at pH 6–7 (optimum, pH 6) and at 15–30 °C (optimum, 25 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the genus , showing sequence similarities of 97.09 % and 96.82 % to DSM 18391 and SP1PR4, respectively. Low gene sequence similarity with members of the genus and different fingerprints with the repetitive primers BOX, ERIC and REP indicated that the isolate represented a novel species of the genus . The major cellular fatty acids were iso-C, C, Cω9, C and summed feature 3 (Cω7/Cω6). The DNA G+C content of strain 03SUJ4 was 63.2±0.1 mol% (mean± of three determinations). The predominant menaquinone was MK-8. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. Several phenotypic characteristics served to differentiate the novel isolate from recognized members of the genus . On the basis of the evidence presented in this study, a novel species, sp. nov. is proposed for strain 03SUJ4 ( = KCTC 23332 = JCM 17517).

Funding
This study was supported by the:
  • National Research Foundation of Korea
  • Ministry of Education, Science and Technology (Award 2010-0006861)
  • National Research Foundation of Korea
  • Korea Government (Award 2012R1A2A2A01015706)
  • National Institute of Biological Resources
  • Ministry of Environment, Republic of Korea
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2013-12-01
2021-10-25
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