1887

Abstract

Strains of Gram-stain-negative, anaerobic, rod-shaped bacteria were isolated from chinchilla () faeces, and strain ST166 was investigated taxonomically. Phylogenetic analyses of 16S rRNA gene sequences revealed that strain ST166 belonged to the genus . Strain ST166 formed a distinct line of descent, and the highest sequence similarity to ST166 was found with JCM 9497 (95.6 %) and JCM 13406 (95.0 %). Analysis of gene sequences also supported these relationships. Based on the phenotypic and phylogenetic characteristics, the novel species sp. nov. is proposed. The type strain of sp. nov. is ST166 ( = JCM 17104 = CCUG 62154).

Funding
This study was supported by the:
  • , Institute for Fermentation, Osaka, Japan , (Award 24570117)
  • , Japan Science and Technology Agency/Japan International Cooperation Agency
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2013-09-01
2020-07-07
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References

  1. Jabari L., Gannoun H., Cayol J.-L., Hedi A., Sakamoto M., Falsen E., Ohkuma M., Hamdi M., Fauque G. & other authors ( 2012 ). Macellibacteroides fermentans gen. nov., sp. nov., a member of the family Porphyromonadaceae isolated from an upflow anaerobic filter treating abattoir wastewaters. . Int J Syst Evol Microbiol 62, 25222527. [CrossRef] [PubMed]
    [Google Scholar]
  2. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  3. Kitahara M., Sakamoto M., Ike M., Sakata S., Benno Y. ( 2005 ). Bacteroides plebeius sp. nov. and Bacteroides coprocola sp. nov., isolated from human faeces. . Int J Syst Evol Microbiol 55, 21432147. [CrossRef] [PubMed]
    [Google Scholar]
  4. Kitahara M., Tsuchida S., Kawasumi K., Amao H., Sakamoto M., Benno Y., Ohkuma M. ( 2011 ). Bacteroides chinchillae sp. nov. and Bacteroides rodentium sp. nov., isolated from chinchilla (Chinchilla lanigera) faeces. . Int J Syst Evol Microbiol 61, 877881. [CrossRef] [PubMed]
    [Google Scholar]
  5. Kitahara M., Sakamoto M., Tsuchida S., Kawasumi K., Amao H., Benno Y., Ohkuma M. ( 2012 ). Bacteroides stercorirosoris sp. nov. and Bacteroides faecichinchillae sp. nov., isolated from chinchilla (Chinchilla lanigera) faeces. . Int J Syst Evol Microbiol 62, 11451150. [CrossRef] [PubMed]
    [Google Scholar]
  6. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [CrossRef]
    [Google Scholar]
  7. Momose Y., Park S. H., Miyamoto Y., Itoh K. ( 2011 ). Design of species-specific oligonucleotide probes for the detection of Bacteroides and Parabacteroides by fluorescence in situ hybridization and their application to the analysis of mouse caecal BacteroidesParabacteroides microbiota. . J Appl Microbiol 111, 176184. [CrossRef] [PubMed]
    [Google Scholar]
  8. Nakano V., Piazza R. M., Cianciarullo A. M., Bueris V., Santos M. F., Menezes M. A., Mendes-Ledesma M. R., Szulczewski V., Elias W. P. & other authors ( 2008 ). Adherence and invasion of Bacteroidales isolated from the human intestinal tract. . Clin Microbiol Infect 14, 955963. [CrossRef] [PubMed]
    [Google Scholar]
  9. Saito H., Miura K. I. ( 1963 ). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72, 619629. [CrossRef] [PubMed]
    [Google Scholar]
  10. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  11. Sakamoto M., Benno Y. ( 2006 ). Reclassification of Bacteroides distasonis, Bacteroides goldsteinii and Bacteroides merdae as Parabacteroides distasonis gen. nov., comb. nov., Parabacteroides goldsteinii comb. nov. and Parabacteroides merdae comb. nov.. Int J Syst Evol Microbiol 56, 15991605. [CrossRef] [PubMed]
    [Google Scholar]
  12. Sakamoto M., Suzuki M., Umeda M., Ishikawa I., Benno Y. ( 2002 ). Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov.. Int J Syst Evol Microbiol 52, 841849. [CrossRef] [PubMed]
    [Google Scholar]
  13. Sakamoto M., Suzuki N., Matsunaga N., Koshihara K., Seki M., Komiya H., Benno Y. ( 2009 ). Parabacteroides gordonii sp. nov., isolated from human blood cultures. . Int J Syst Evol Microbiol 59, 28432847. [CrossRef] [PubMed]
    [Google Scholar]
  14. Sakamoto M., Suzuki N., Benno Y. ( 2010 ). hsp60 and 16S rRNA gene sequence relationships among species of the genus Bacteroides with the finding that Bacteroides suis and Bacteroides tectus are heterotypic synonyms of Bacteroides pyogenes . . Int J Syst Evol Microbiol 60, 29842990. [CrossRef] [PubMed]
    [Google Scholar]
  15. Stackebrandt E., Ebers J. ( 2006 ). Taxonomic parameters revisited: tarnished gold standards. . Microbiology Today 33, 152155.
    [Google Scholar]
  16. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [CrossRef] [PubMed]
    [Google Scholar]
  17. Tan H. Q., Li T.-T., Zhu C., Zhang X.-Q., Wu M., Zhu X.-F. ( 2012 ). Parabacteroides chartae sp. nov., an obligately anaerobic species from wastewater of a paper mill. . Int J Syst Evol Microbiol 62, 26132617. [CrossRef] [PubMed]
    [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
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