1887

Abstract

A novel Gram-stain-positive, non-motile, rod-shaped bacterium, designated 5GHs34-4, was isolated from greenhouse soil in Yongin, Republic of Korea. Growth occurred in the temperature range of 10–37 °C (optimum 28–30 °C) and at pH 5.0–9.0 (optimum pH 7.0). It can tolerate up to 3 % (w/v) NaCl. The strain showed 16S rRNA gene sequence similarity levels of 95.1–97.0 % with species of the genus , 95.7–96.7 % with species of the genus , 95.1–96.4 % with species of the genus and 96.1 % with and . The highest sequence similarities (97.0 %) were with JCM 1368, VKM Ac-1401 and LI1. The peptidoglycan type determined for strain 5GHs34-4 was B2γ with -2,4-diaminobutyric acid at position 3. The murein was of the acetyl type. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The menaquinones detected were MK-13, MK-12 and MK-14, and the major fatty acids were summed feature 8 (Cω7 and/or Cω6), anteiso-C and anteiso-C. The phenotypic and phylogenetic traits of strain 5GHs34-4 differed in some respects from those of members of the family . Therefore, strain 5GHs34-4 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is 5GHs34-4 ( = KACC 15523 = NBRC 108754).

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2013-11-01
2020-01-27
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