1887

Abstract

The taxonomic position of strain MSSRFBL1, isolated from chickpea rhizosphere soil from Kannivadi, India, was determined. Strain MSSRFBL1 formed bluish black colonies, stained Gram-negative and was motile, aerobic, capable of fixing dinitrogen, oxidase-negative and catalase-positive. Q-10 was the major respiratory quinone. Major fatty acids of strain MSSRFBL1 were Cω7 and Ccycloω8. Minor amounts of C, C, C 3-OH, C 3-OH, C, Cω6/Cω7, C 3-OH and Cω7 were also present. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylcholine and two unidentified glycolipids. Bacteriohopane derivatives (BHD1 and 2), diplopterol, diploptene, bishomohopanediol, adenosylhopane and 2β-methyl bacteriohopanetetrol were the major hopanoids of strain MSSRFBL1. The genomic DNA G+C content was 71 mol%. EzTaxon-e-based analysis of the 16S rRNA gene indicated the highest similarity of strain MSSRFBL1 to LMG 20216 (97.3 %) and other members of the genus (<96.9 %) in the family of the class . However, phylogenetic analysis based on 16S rRNA, , and gene sequences showed distinct out-grouping from the recognized genera of the family . Based on phenotypic, genotypic and chemotaxonomic characters, strain MSSRFBL1 represents a novel species in a new genus in the family for which the name gen. nov., sp. nov. is proposed. The type strain of is MSSRFBL1 ( = DSM 25528 = KCTC 32403).

Funding
This study was supported by the:
  • , Government of India
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2013-12-01
2020-07-08
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