1887

Abstract

Bacterial strain SF87, and additional strains SF80, SF362 and 106-C, isolated from the nematode , are non-bioluminescent Gram-reaction-negative bacteria that share many of the carbohydrate fermentation reactions recorded for the type strains of recognized species. Based on 16S rRNA gene sequence data, strain SF87 is shown to be closely related (98 % similarity) to DSM 17903. Nucleotide sequences of strain SF87 obtained from the , , , and genes showed 96–97 % similarity with DSM 17902 However, strain SF87 shares only 52.7 % DNA–DNA relatedness with the type strain of , confirming that it belongs to a different species. Strains SF87, SF80, SF362 and 106-C are phenotypically similar to and , except that they do not produce acid from aesculin. These strains are thus considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SF87 ( = DSM 25463 = ATCC BAA-2406).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.049049-0
2013-09-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/9/3220.html?itemId=/content/journal/ijsem/10.1099/ijs.0.049049-0&mimeType=html&fmt=ahah

References

  1. Akhurst R. J.. ( 1980;). Morphological and functional dimorphism in Xenorhabdus spp., bacteria symbiotically associated with the insect pathogenic nematodes Neoaplectana and Heterorhabditis. . J Gen Microbiol 121:, 303–309.
    [Google Scholar]
  2. Akhurst R. J., Boemare N. E.. ( 1988;). A numerical taxonomic study of the genus Xenorhabdus (Enterobacteriaceae) and proposed elevation of the subspecies of X. nematophilus to species. . J Gen Microbiol 134:, 1835–1845.[PubMed]
    [Google Scholar]
  3. Boemare N. E., Akhurst R. J.. ( 1988;). Biochemical and physiological characterization of colony form variants in Xenorhabdus spp. (Enterobacteriaceae). . J Gen Microbiol 134:, 751–761.
    [Google Scholar]
  4. Brunel B., Givaudan A., Lanois A., Akhurst R. J., Boemare N.. ( 1997;). Fast and accurate identification of Xenorhabdus and Photorhabdus species by restriction analysis of PCR-amplified 16S rRNA genes. . Appl Environ Microbiol 63:, 574–580.[PubMed]
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Fe lsenstein J.. ( 2005;). phylip (phylogeny inference package), version 3.6. . Distributed by the author. Department of Genome Sciences, University of Washington;, Seattle, USA:.
  7. Ferreira T., Van Reenen C. A., Pagès S., Tailliez P., Malan A. P., Dicks L. M. T.. ( 2013;). Photorhabdus luminescens subsp. noenieputensis subsp. nov., a symbiotic bacterium associated with a new Heterorhabditis species related to Heterorhabditis indica. . Int J Syst Evol Microbiol 63:, 1853–1858. [CrossRef][PubMed]
    [Google Scholar]
  8. Huss V. A. R., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef][PubMed]
    [Google Scholar]
  9. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  10. Kuwata R., Qiu L., Wang W., Harada Y., Yoshida M., Kondo E., Yoshiga T.. ( 2013;). Xenorhabdus ishibashii sp. nov., isolated from the entomopathogenic nematode Steinernema aciari. . Int J Syst Evol Microbiol 63:, 1690–1695. [CrossRef][PubMed]
    [Google Scholar]
  11. Lee M. M., Stock S. P.. ( 2010;). A multigene approach for assessing evolutionary relationships of Xenorhabdus spp. (γ-Proteobacteria), the bacterial symbionts of entomopathogenic Steinernema nematodes. . J Invertebr Pathol 104:, 67–74. [CrossRef][PubMed]
    [Google Scholar]
  12. Lengyel K., Lang E., Fodor A., Szállás E., Schumann P., Stackebrandt E.. ( 2005;). Description of four novel species of Xenorhabdus, family Enterobacteriaceae: Xenorhabdus budapestensis sp. nov., Xenorhabdus ehlersii sp. nov., Xenorhabdus innexi sp. nov., and Xenorhabdus szentirmaii sp. nov.. Syst Appl Microbiol 28:, 115–122. [CrossRef][PubMed]
    [Google Scholar]
  13. Malan A. P., Nguyen K. B., Addison M. F.. ( 2006;). Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from the southwestern parts of South Africa. . Afr Plant Prot 12:, 65–69.
    [Google Scholar]
  14. Malan A. P., Knoetze R., Moore S. D.. ( 2011;). Isolation and identification of entomopathogenic nematodes from citrus orchards in South Africa and their biocontrol potential against false codling moth. . J Invertebr Pathol 108:, 115–125. [CrossRef][PubMed]
    [Google Scholar]
  15. Nguyen K. B., Malan A. P., Gozel U.. ( 2006;). Steinernema khoisanae n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from South Africa. . Nematology 8:, 157–175. [CrossRef]
    [Google Scholar]
  16. Nishimura Y., Hagiwara A., Suzuki T., Yamanaka S.. ( 1994;). Xenorhabdus japonicus sp. nov. associated with the nematode Steinernema kushidai. . World J Microbiol Biotechnol 10:, 207–210. [CrossRef]
    [Google Scholar]
  17. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  18. Somogyi E., Saskoi A., Furgani G., Lengyel K., Mathe A., Fodor A.. ( 2002;). Isolation and characterization of bacterial symbionts of Steinernema strains of long dauer phenotype. . http://www.cost850.ch/publications/20020404_debrecen/20020404_06.pdf
  19. Somvanshi V. S., Lang E., Ganguly S., Swiderski J., Saxena A. K., Stackebrandt E.. ( 2006;). A novel species of Xenorhabdus, family Enterobacteriaceae: Xenorhabdus indica sp. nov., symbiotically associated with entomopathogenic nematode Steinernema thermophilum Ganguly and Singh, 2000. . Syst Appl Microbiol 29:, 519–525. [CrossRef][PubMed]
    [Google Scholar]
  20. Tailliez P., Pagès S., Ginibre N., Boemare N.. ( 2006;). New insight into diversity in the genus Xenorhabdus, including the description of ten novel species. . Int J Syst Evol Microbiol 56:, 2805–2818. [CrossRef][PubMed]
    [Google Scholar]
  21. Tailliez P., Laroui C., Ginibre N., Paule A., Pagès S., Boemare N.. ( 2010;). Phylogeny of Photorhabdus and Xenorhabdus based on universally conserved protein-coding sequences and implications for the taxonomy of these two genera. Proposal of new taxa: X. vietnamensis sp. nov., P. luminescens subsp. hainanensis subsp. nov., P. temperata subsp. tasmaniensis subsp. nov., and the reclassification of P. luminescens subsp. thracensis as P. temperata subsp. thracensis comb. nov.. Int J Syst Evol Microbiol 60:, 1921–1937. [CrossRef][PubMed]
    [Google Scholar]
  22. Tailliez P., Pagès S., Edgington S., Tymo L. M., Buddie A. G.. ( 2012;). Description of Xenorhabdus magdalenensis sp. nov., the symbiotic bacterium associated with Steinernema australe. . Int J Syst Evol Microbiol 62:, 1761–1765. [CrossRef][PubMed]
    [Google Scholar]
  23. Thomas G. M., Poinar G. O.. ( 1979;). Xenorhabdus gen. nov., a genus of entomopathogenic, nematophilic bacteria of the family Enterobacteriaceae. . Int J Syst Bacteriol 29:, 352–360. [CrossRef]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.049049-0
Loading
/content/journal/ijsem/10.1099/ijs.0.049049-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error