1887

Abstract

A Gram-stain-positive, aerobic, non-motile, rod-shaped bacterium, designated strain CF5-4, was isolated from iron mining powder. 16S rRNA gene sequence analysis grouped strain CF5-4 in a single cluster with DSM 3133 (97.6 % similarity). The major fatty acids (>5 %) of strain CF5-4 were anteiso-C, anteiso-C A, C, iso-C, iso-C and anteiso-C. The predominant respiratory quinone was MK-10(H) and the genomic DNA G+C content was 74.7 mol%. The major polar lipids were diphosphatidylglycerol and one unidentified phosphoglycolipid. The peptidoglycan type of strain CF5-4 was A4β, containing -Orn–-Ser–-Asp. The cell-wall sugars were rhamnose, fucose, mannose and galactose. The results of DNA–DNA hybridization in combination with the comparison of phenotypic and phylogenetic characteristics among strain CF5-4 and related micro-organisms revealed that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CF5-4 ( = KCTC 29134 = CCTCC AB2012198).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 31170106)
  • , National High Tech Development Program , (Award 2012AA101402-3)
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2013-09-01
2020-05-27
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References

  1. Abt B., Foster B., Lapidus A., Clum A., Sun H., Pukall R., Lucas S., Glavina Del Rio T., Nolan M. & other authors ( 2010 ). Complete genome sequence of Cellulomonas flavigena type strain (134T). . Stand Genomic Sci 3, 1525. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bagnara C., Toci R., Gaudin C., Belaich J. P. ( 1985 ). Isolation and characterization of a cellulolytic microorganism, Cellulomonas fermentans sp. nov.. Int J Syst Bacteriol 35, 502507. [CrossRef]
    [Google Scholar]
  3. Breznak J. A., Costilow R. N. ( 1994 ). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  4. Cappuccino J. G., Sherman N. ( 2002 ). Microbiology: a Laboratory Manual, , 6th edn.. Menlo Park, CA:: Benjamin/Cummings;.
    [Google Scholar]
  5. Chen F., Shi Z., Wang G. ( 2012 ). Arenimonas metalli sp. nov., isolated from an iron mine. . Int J Syst Evol Microbiol 62, 17441749. [CrossRef] [PubMed]
    [Google Scholar]
  6. Cowan S. T., Steel K. J. ( 1965 ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  7. Dussault H. P. ( 1955 ). An improved technique for staining red halophilic bacteria. . J Bacteriol 70, 484485.[PubMed]
    [Google Scholar]
  8. Fan H., Su C., Wang Y., Yao J., Zhao K., Wang Y., Wang G. ( 2008 ). Sedimentary arsenite-oxidizing and arsenate-reducing bacteria associated with high arsenic groundwater from Shanyin, Northwestern China. . J Appl Microbiol 105, 529539. [CrossRef] [PubMed]
    [Google Scholar]
  9. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  10. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [CrossRef]
    [Google Scholar]
  11. Guindon S., Lethiec F., Duroux P., Gascuel O. ( 2005 ). phyml Online–a web server for fast maximum likelihood-based phylogenetic inference. . Nucleic Acids Res 33 (Web Server issue), W557W559. [CrossRef] [PubMed]
    [Google Scholar]
  12. Hugh R., Leifson E. ( 1953 ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. . J Bacteriol 66, 2426.[PubMed]
    [Google Scholar]
  13. Huss V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [CrossRef] [PubMed]
    [Google Scholar]
  14. Jones B. E., Grant W. D., Duckworth A. W., Schumann P., Weiss N., Stackebrandt E. ( 2005 ). Cellulomonas bogoriensis sp. nov., an alkaliphilic cellulomonad. . Int J Syst Evol Microbiol 55, 17111714. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kroppenstedt R. M. ( 1985 ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics (SAB Technical Series no. 20), pp. 173199. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  17. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [CrossRef]
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  19. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [CrossRef]
    [Google Scholar]
  20. Ryu E. ( 1938 ). On the Gram-differentiation of bacteria by the simplest method. . J Jpn Soc Vet Sci 17, 5863. [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  22. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  23. Schumann P. ( 2011 ). Peptidoglycan structure. . In Taxonomy of Prokaryotes, Methods in Microbiology, vol. 38, pp. 101129. Edited by Rainey F., Oren A. . London:: Academic Press;. [CrossRef]
    [Google Scholar]
  24. Shi Z., Luo G., Wang G. ( 2012 ). Cellulomonas carbonis sp. nov., isolated from coal mine soil. . Int J Syst Evol Microbiol 62, 20042010. [CrossRef] [PubMed]
    [Google Scholar]
  25. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  26. Stackebrandt E., Prauser H. ( 1991 ). The family Cellulomonadaceae . . In The Prokaryotes, pp. 13231345. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. . New York:: Springer;.
    [Google Scholar]
  27. Stackebrandt E., Schumann P., Prauser H. ( 2006 ). The family Cellulomonadaceae . . In The Prokaryotes, , 3rd edn., pp. 9831001. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  28. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [CrossRef] [PubMed]
    [Google Scholar]
  29. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  30. Tindall B. J. ( 1990 ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [CrossRef]
    [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
  32. Yi H., Schumann P., Chun J. ( 2007 ). Demequina aestuarii gen. nov., sp. nov., a novel actinomycete of the suborder Micrococcineae, and reclassification of Cellulomonas fermentans Bagnara et al. 1985 as Actinotalea fermentans gen. nov., comb. nov.. Int J Syst Evol Microbiol 57, 151156. [CrossRef] [PubMed]
    [Google Scholar]
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