1887

Abstract

An actinobacterial strain, designated ViU22, was isolated from a natural uranium-rich soil and was studied using a polyphasic approach. Cells formed orange-pigmented colonies, were rod-shaped, Gram-positive (non-staining method), non-motile and non-spore-forming. This organism grew in 0–4.5 % (w/v) NaCl and at 15–37 °C, with optimal growth occurring in 0.5 % (w/v) NaCl and at 30 °C. Comparative 16S rRNA gene sequence analysis revealed that the strain ViU22 belonged to the genus . It exhibited highest 16S rRNA gene sequence similarity with the type strains of (98.14 %) and (98.02 %). The DNA–DNA relatedness of strains ViU22 with the most closely related type strains and DSM 19164 was 20.10 % (±0.70) and 28.05 % (±0.35), respectively. Strain ViU22 possessed a type B2β peptidoglycan with partial substitution of glutamic acid by 3-hydroxy glutamic acid. The major menaquinones were MK-11 and MK-12. Major polar lipids detected in the strain ViU22 were diphosphatidylglycerol, phosphatidylglycerol, an unknown phospholipid and unknown glycolipids. The predominant fatty acids were anteiso-C, anteiso-C and iso-C, a pattern reported for other species. The major cell-wall sugars were galactose, xylose and mannose and the DNA G+C content was 71 mol%. Together, the DNA–DNA hybridization results and the differentiating phenotypic characteristics, showed that strain ViU22 should be classified as the type strain of a novel species within the genus , for which the name sp. nov. is proposed. The type strain is ViU22 ( = ATCC BAA-2396 = CCUG 62198 = DSM 25044).

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2013-07-01
2019-12-09
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