1887

Abstract

A novel Gram-staining-negative, strictly aerobic bacterium, designated BS14, was isolated from a marine tidal flat of the South Sea in Korea. Colonies were opaque, white, smooth and circular on marine agar. Cells were moderately halophilic, non-motile rods showing catalase- and oxidase-positive reactions. Growth of strain BS14 was observed at 5–40 °C (optimum: 30 °C), pH 6.5–9.5 (optimum: 7.0–7.5) and 0–10 % (w/v) NaCl (optimum: 1–1.5 %). The G+C content of the genomic DNA was 61.6 mol%. Strain BS14 contained ubiquinone-10 (Q-10) as the sole respiratory quinone and summed feature 8 (comprising Cω7 and/or Cω6), C 3-OH, C 3-OH and C as the major fatty acids. The polar lipid pattern comprised phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminolipid, an unidentified phospholipid and an unidentified polar lipid. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BS14 formed a tight phylogenetic lineage with D9-3 with a bootstrap value of 100 %. The 16S rRNA gene sequence similarity between strain BS14 and D9-3 was 97.4 % and their DNA–DNA relatedness was 19.1±3.6 %. Based on the phenotypic and genotypic studies, strain BS14 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BS14 ( = KACC 16442 = JCM 18630). An emended description of the genus Foesel 2011 is also proposed.

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2013-08-01
2020-01-22
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References

  1. Bernardet J.-F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  2. DeSantis T. Z. Jr, Hugenholtz P., Keller K., Brodie E. L., Larsen N., Piceno Y. M., Phan R., Andersen G. L.. ( 2006;). NAST: a multiple sequence alignment server for comparative analysis of 16S rRNA genes. . Nucleic Acids Res 34: (Web Server issue), W394–W399. [CrossRef][PubMed]
    [Google Scholar]
  3. Einen J., Øvreås L.. ( 2006;). Flaviramulus basaltis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seafloor basalt. . Int J Syst Evol Microbiol 56:, 2455–2461. [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J.. ( 2002;). phylip (phylogeny inference package), version 3.68. Distributed by the author. . Department of Genome Sciences, University of Washington;, Seattle, USA:.
  5. Foesel B. U., Drake H. L., Schramm A.. ( 2011;). Defluviimonas denitrificans gen. nov., sp. nov., and Pararhodobacter aggregans gen. nov., sp. nov., non-phototrophic Rhodobacteraceae from the biofilter of a marine aquaculture. . Syst Appl Microbiol 34:, 498–502. [CrossRef][PubMed]
    [Google Scholar]
  6. Garrity G. M., Bell J. A., Lilburn T.. ( 2005;). Family I. Rhodobacteraceae. . In: Bergey’s Manual of Systematic Bacteriology, vol. 2, p. 161. Edited by Garrity G. M., Brenner D. J., Krieg N. R., Staley J. T... New York:: Springer;.
    [Google Scholar]
  7. Gomori G.. ( 1955;). Preparation of buffers for use in enzyme studies. . In Methods in Enzymology, vol. 1, pp. 138–146. Edited by Colowick S. P., Kaplan N. O... New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  8. Gonzalez J. M., Saiz-Jimenez C.. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4:, 770–773. [CrossRef][PubMed]
    [Google Scholar]
  9. Isnansetyo A., Kamei Y.. ( 2003;). Pseudoalteromonas phenolica sp. nov., a novel marine bacterium that produces phenolic anti-methicillin-resistant Staphylococcus aureus substances. . Int J Syst Evol Microbiol 53:, 583–588. [CrossRef][PubMed]
    [Google Scholar]
  10. Jeon C. O., Park W., Ghiorse W. C., Madsen E. L.. ( 2004;). Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. . Int J Syst Evol Microbiol 54:, 93–97. [CrossRef][PubMed]
    [Google Scholar]
  11. Jeong S. H., Yang S. H., Jin H. M., Kim J. M., Kwon K. K., Jeon C. O.. ( 2013;). Microbulbifer gwangyangensis sp. nov. and Microbulbifer pacificus sp. nov., isolated from marine environments. . Int J Syst Evol Microbiol 63:, 1335–1341. [CrossRef][PubMed]
    [Google Scholar]
  12. Jin H. M., Lee H. J., Kim J. M., Park M. S., Lee K., Jeon C. O.. ( 2011;). Litorimicrobium taeanense gen. nov., sp. nov., isolated from a sandy beach. . Int J Syst Evol Microbiol 61:, 1392–1396. [CrossRef][PubMed]
    [Google Scholar]
  13. Jin H. M., Kim J. M., Lee H. J., Madsen E. L., Jeon C. O.. ( 2012;). Alteromonas as a key agent of polycyclic aromatic hydrocarbon biodegradation in crude oil-contaminated coastal sediment. . Environ Sci Technol 46:, 7731–7740. [CrossRef][PubMed]
    [Google Scholar]
  14. Jung J. Y., Kim J. M., Jin H. M., Kim S. Y., Park W., Jeon C. O.. ( 2011;). Litorimonas taeanensis gen. nov., sp. nov., isolated from a sandy beach. . Int J Syst Evol Microbiol 61:, 1534–1538. [CrossRef][PubMed]
    [Google Scholar]
  15. Kim J. M., Lee S. H., Jung J. Y., Jeon C. O.. ( 2010a;). Marinobacterium lutimaris sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 60:, 1828–1831. [CrossRef][PubMed]
    [Google Scholar]
  16. Kim J. M., Lee H. J., Kim S. Y., Song J. J., Park W., Jeon C. O.. ( 2010b;). Analysis of fine-scale population structure of ‘Candidatus Accumulibacter phosphatis’ in enhanced biological phosphorus removal sludge using fluorescence in situ hybridization and flow cytometric sorting. . Appl Environ Microbiol 76:, 3825–3835. [CrossRef][PubMed]
    [Google Scholar]
  17. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  18. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  19. Lee S. H., Shim J. K., Kim J. M., Choi H.-K., Jeon C. O.. ( 2011;). Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella. . Int J Syst Evol Microbiol 61:, 722–727. [CrossRef][PubMed]
    [Google Scholar]
  20. Leifson E.. ( 1963;). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  21. Lu S., Park M., Ro H.-S., Lee D. S., Park W., Jeon C. O.. ( 2006;). Analysis of microbial communities using culture-dependent and culture-independent approaches in an anaerobic/aerobic SBR reactor. . J Microbiol 44:, 155–161.[PubMed]
    [Google Scholar]
  22. Lyman J., Fleming R. H.. ( 1940;). Composition of sea water. . J Mar Res 3:, 134–146.
    [Google Scholar]
  23. Math R. K., Jeong S. H., Jin H. M., Park M. S., Kim J. M., Jeon C. O.. ( 2012a;). Kordiimonas aestuarii sp. nov., a marine bacterium isolated from a tidal flat. . Int J Syst Evol Microbiol 62:, 3049–3054. [CrossRef][PubMed]
    [Google Scholar]
  24. Math R. K., Jin H. M., Kim J. M., Hahn Y., Park W., Madsen E. L., Jeon C. O.. ( 2012b;). Comparative genomics reveals adaptation by Alteromonas sp. SN2 to marine tidal-flat conditions: cold tolerance and aromatic hydrocarbon metabolism. . PLoS ONE 7:, e35784. [CrossRef][PubMed]
    [Google Scholar]
  25. McCammon S. A., Bowman J. P.. ( 2000;). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov.. Int J Syst Evol Microbiol 50:, 1055–1063. [CrossRef][PubMed]
    [Google Scholar]
  26. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  27. Rosselló-Mora R., Amann R.. ( 2001;). The species concept for prokaryotes. . FEMS Microbiol Rev 25:, 39–67. [CrossRef][PubMed]
    [Google Scholar]
  28. Sambrook J., Russell D. W.. ( 2001;). Molecular Cloning: a Laboratory Manual, , 3rd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  29. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  30. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  31. Stamatakis A., Ott M., Ludwig T.. ( 2005;). RAxML-OMP: An efficient program for phylogenetic inference on SMPs. . In Proceedings of 8th International Conference on Parallel Computing Technologies (PaCT2005), Lecture Notes in Computer Science, 3506, 288–302, Springer.
    [Google Scholar]
  32. Wang G., Skipper H. D.. ( 2004;). Identification of denitrifying rhizobacteria from bentgrass and bermudagrass golf greens. . J Appl Microbiol 97:, 827–837. [CrossRef][PubMed]
    [Google Scholar]
  33. Winberg P. C., Lynch T. P., Murray A., Jones A. R., Davis A. R.. ( 2007;). The importance of spatial scale for the conservation of tidal flat macrobenthos: An example from New South Wales, Australia. . Biol Conserv 134:, 310–320. [CrossRef]
    [Google Scholar]
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