1887

Abstract

Pink-pigmented, facultatively methylotrophic bacteria, strains 87e and 99b, were isolated from the bryophytes and , respectively. The cells of both strains were Gram-reaction-negative, motile, non-spore-forming rods. On the basis of 16S rRNA gene sequence similarity, strains 87e and 99b were found to be related to ATCC 27886 (97.1 % and 97.7 %, respectively). Strains 87e and 99b showed highest 16S rRNA gene similarity to 23e (98.3 and 99.0 %, respectively). The phylogenetic similarities to all other species of the genus with validly published names were less than 97 %. Major cellular fatty acids of both strains were Cω7 and C. The results of DNA–DNA hybridization, phylogenetic analyses based on 16S rRNA and 60 gene sequences, fatty acid profiles, whole-cell matrix-assisted, laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains 87e and 99b from their phylogenetically closest relatives. We propose that strains 87e and 99b represent novel species within the genus , for which the names sp. nov. (type strain 87e = DSM 24195 = NBRC 107714) and sp. nov. (type strain 99b = DSM 24105 = NBRC 107710) are proposed.

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2013-09-01
2019-10-22
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