1887

Abstract

A Gram-positive filamentous bacterial strain that developed large campanulate sporangia at the ends of sporangiophores on substrate mycelium was isolated from bamboo forest soil in Thailand. According to the results of a polyphasic taxonomic study, our isolate had typical characteristics of members of the genus . The 16S rRNA gene sequence analysis also indicated that strain A-T 6646 belonged to the genus , being most closely related to DSM 43865 (97.61 %) and NBRC 14524 (97.52 %). The DNA–DNA relatedness values, which differentiate the new strain from the most closely related species, were significantly below 70 %. The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars contained xylose and arabinose. The predominant menaquinone was MK-9(H). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were iso-C, anteiso-C, iso-C and anteiso-C. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, the isolate is proposed to represent a novel species to be named sp. nov. The type strain is A-T 6646 ( = BCC 46194 = NBRC 109076).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.048017-0
2013-08-01
2019-09-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/8/3037.html?itemId=/content/journal/ijsem/10.1099/ijs.0.048017-0&mimeType=html&fmt=ahah

References

  1. Arai T.. ( 1975;). Culture Media for Actinomycetes. Tokyo:: The Society for Actinomycetes Japan;.
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  3. Couch J. N.. ( 1950;). Actinoplanes, a new genus of the Actinomycetales. . J Elisha Mitchell Sci Soc 66:, 87–92.
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  5. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. ( 1983;). Parsimony in systematics: biological and statistical issues. . Annu Rev Ecol Syst 14:, 313–333. [CrossRef]
    [Google Scholar]
  7. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Goodfellow M., Stanton L. J., Simpson K. E., Minnikin D. E.. ( 1990;). Numerical and chemical classification of Actinoplanes and some related actinomycetes. . J Gen Microbiol 136:, 19–36. [CrossRef]
    [Google Scholar]
  9. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N.. ( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  10. Greenwood J. R., Pickett M. J.. ( 1979;). Salient features of Haemophilus vaginalis. . J Clin Microbiol 9:, 200–204.[PubMed]
    [Google Scholar]
  11. Itoh T., Kudo T., Parenti F., Seino A.. ( 1989;). Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. . Int J Syst Bacteriol 39:, 168–173. [CrossRef]
    [Google Scholar]
  12. Kämpfer P., Kroppenstedt R. M.. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  13. Katsura K., Kawasaki H., Potacharoen W., Saono S., Seki T., Yamada Y., Uchimura T., Komagata K.. ( 2001;). Asaia siamensis sp. nov., an acetic acid bacterium in the α-proteobacteria. . Int J Syst Evol Microbiol 51:, 559–563.[PubMed]
    [Google Scholar]
  14. Kawasaki H., Hoshino Y., Hirata A., Yamasato K.. ( 1993;). Is intracytoplasmic membrane structure a generic criterion? It does not coincide with phylogenetic interrelationships among phototrophic purple nonsulfur bacteria. . Arch Microbiol 160:, 358–362. [CrossRef][PubMed]
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  16. Komagata K., Suzuki K. I.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  17. Lechevalier M. P., Lechevalier H.. ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  18. Lechevalier M. P., De Bievre C., Lechevalier H.. ( 1977;). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5:, 249–260. [CrossRef]
    [Google Scholar]
  19. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  20. Nonomura H., Hayakawa M.. ( 1988;). New methods for the selective isolation of soil actinomycetes. . In Biology of Actinomycetes, pp. 288–293. Edited by Okami Y., Beppu T., Ogawara H... Tokyo, Japan:: Japan Scientific Societies Press;.
    [Google Scholar]
  21. Saito H., Miura K. I.. ( 1963;). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72:, 619–629. [CrossRef][PubMed]
    [Google Scholar]
  22. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  23. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI, Inc;.
  24. Sazak A., Sahin N., Camas M.. ( 2012;). Actinoplanes abujensis sp. nov., isolated from Nigerian arid soil. . Int J Syst Evol Microbiol 62:, 960–965. [CrossRef][PubMed]
    [Google Scholar]
  25. Shirling E. B., Gottlieb D.. ( 1966;). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  26. Staneck J. L., Roberts G. D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  27. Suriyachadkun C., Chunhametha S., Thawai C., Tamura T., Potacharoen W., Kirtikara K., Sanglier J. J.. ( 2009;). Planotetraspora thailandica sp. nov., isolated from soil in Thailand. . Int J Syst Evol Microbiol 59:, 992–997. [CrossRef][PubMed]
    [Google Scholar]
  28. Suzuki S. I.. ( 2001;). Establishment and use of gellan gum media for selective isolation and distribution survey of specific rare actinomycetes. . Actinomycetologica 15:, 55–60. [CrossRef]
    [Google Scholar]
  29. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  30. Tamura T., Hatano K.. ( 2001;). Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov.. Int J Syst Evol Microbiol 51:, 2119–2125. [CrossRef][PubMed]
    [Google Scholar]
  31. Tamura T., Nakagaito Y., Nishii T., Hasegawa T., Stackebrandt E., Yokota A.. ( 1994;). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov.. Int J Syst Bacteriol 44:, 193–203. [CrossRef][PubMed]
    [Google Scholar]
  32. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  33. Uchida K., Aida K.. ( 1984;). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. . J Gen Appl Microbiol 30:, 131–134. [CrossRef]
    [Google Scholar]
  34. Verlander C. P.. ( 1992;). Detection of horseradish peroxidase by colorimetry. . In Nonisotopic DNA Probe Techniques, pp. 185–201. Edited by Kricka L. J... New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  35. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  36. Williams S. T., Cross T.. ( 1971;). Actinomycetes. . Methods Microbiol 4:, 295–334. [CrossRef]
    [Google Scholar]
  37. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178.
    [Google Scholar]
  38. Yamada Y., Katsura K., Kawasaki H., Widyastuti Y., Saono S., Seki T., Uchimura T., Komagata K.. ( 2000;). Asaia bogorensis gen. nov., sp. nov., an unusual acetic acid bacterium in the α-Proteobacteria. . Int J Syst Evol Microbiol 50:, 823–829. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.048017-0
Loading
/content/journal/ijsem/10.1099/ijs.0.048017-0
Loading

Data & Media loading...

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error