1887

Abstract

A strictly anaerobic, Gram-stain-negative, non-spore-forming, motile bacterium, designated strain FSRU B0405, was isolated from a commercial pickle spoilage tank and characterized by biochemical, physiological and molecular biological methods. Analyses of the 16S rRNA gene sequence of strain FSRU B0405 showed affiliation to the class in the phylum , with the closest relatives being the type strains of (96 %) and (95 %). In maximum-likelihood and neighbour-joining phylogenetic trees, strain FSRU B0405 clustered definitively (in 100 % of bootstrapped trees) within the genus , but not specifically with any characterized species within this genus. Strain FSRU B0405 was a slightly curved rod, varying from 3 to 30 µm in length, motile with a distinctive X-wise movement, having flagella only on the concave side of the cell. The isolate produced acetate and propionate from fructose and glucose as major metabolites similar to type strains of species of the genus . The major fatty acids were C, C, C, C 3-OH, C and Cω11. Strain FSRU B0405 differed from the pickle wastewater strain, TY, due to its lack of susceptibility to vancomycin, acetoin production, growth temperature range, acid production from adonitol, erythritol, glycerol, inositol, lactose, maltose, mannose, ribose, salicin, sorbitol, trehalose and xylitol and lack of hydrolysis of milk. Strain FSRU B0405 could be differentiated from other species of the genus both phenotypically and genetically. The results indicate that strain FSRU B0405 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FSRU B0405 ( = ATCC BAA-2501 = VTT E-113163). An emended description of the genus is also provided.

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2013-10-01
2019-10-20
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References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Chihib N. , Monnerat L. , Membre J. M. , Tholozan J. L. . ( 1999; ). Nisin, temperature and pH effects on growth and viability of Pectinatus frisingensis, a Gram-negative, strictly anaerobic beer-spoilage bacterium. . J Appl Microbiol 87:, 438–446. [CrossRef] [PubMed]
    [Google Scholar]
  3. Fleming H. P. , Daeschel M. A. , McFeeters R. F. , Pierson M. D. . ( 1989; ). Butyric acid spoilage of fermented cucumbers. . J Food Sci 54:, 636–639. [CrossRef]
    [Google Scholar]
  4. Fleming H. P. , McDonald L. C. , McFeeters R. F. , Thompson R. L. , Humphries E. G. . ( 1995; ). Fermentation of cucumbers without sodium chloride. . J Food Sci 60:, 312–315. [CrossRef]
    [Google Scholar]
  5. Franco W. , Pérez-Díaz I. M. , Johanningsmeier S. D. , McFeeters R. F. . ( 2012; ). Characteristics of spoilage-associated secondary cucumber fermentation. . Appl Environ Microbiol 78:, 1273–1284. [CrossRef] [PubMed]
    [Google Scholar]
  6. Gonzalez J. M. , Jurado V. , Laiz L. , Zimmermann J. , Hermosin B. , Sainz-Jimenez C. . ( 2004; ). Pectinatus portalensis sp. nov. In Validation of publication of new names and new combinations previously effectively published outside the IJSEM, List no. 102. . Int J Syst Evol Microbiol 55:, 547–549.[PubMed]
    [Google Scholar]
  7. Haakensen M. C. , Butt L. , Chaban B. , Deneer H. , Ziola B. . ( 2007; ). horA-Specific real-time PCR for detection of beer-spoilage lactic acid bacteria. . J Am Soc Brew Chem 65:, 157–165.
    [Google Scholar]
  8. Haikara A. , Penttila L. , Enari T.-M , Lounatmaa K. . ( 1981; ). Microbiological, biochemical, and electron microscopic characterization of a Pectinatus strain. . Appl. Environ. Microbiol. 41, 511–517.
    [Google Scholar]
  9. Haikara A. , Helander I. M. . ( 1995; ). Cellular fatty acyl and alkenyl residues in Megasphaera and Pectinatus species: contrasting profiles and detection of beer spoilage. . Microbiology 141:, 1131–1137. [CrossRef]
    [Google Scholar]
  10. Haikara A. , Helander I. . ( 2006;). Pectinatus, Megashaera and Zymophilus . . In The Prokaryotes , , 3rd edn..vol.4 pp. 965–981. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K.-H. , Stackebrandt E. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  11. Haikara A. , Juvonen R. . ( 2009; ). Genus XV. Pectinatus . . In Bergey's Manual of Systematic Bacteriology, , 2nd edn.. pp. 1094–1099. Edited by De Vos P. , Garrity G. , Jones D. , Krieg N. R. , Ludwig W. , Rainey F. A. , Schleifer K.-H. , Whitman W. B. . . New York:: Springer;.
    [Google Scholar]
  12. Helander I. M. , Kilpeläinen I. , Vaara M. , Moran A. P. , Lindner B. , Seydel U. . ( 1994; ). Chemical structure of the lipid A component of lipopolysaccharides of the genus Pectinatus . . Eur J Biochem 224:, 63–70. [CrossRef] [PubMed]
    [Google Scholar]
  13. Holdeman L. V. , Cato E. P. , Moore W. E. C. . ( 1977; ). Anaerobe Laboratory Manual, , 4th edn.. Blacksburg, VA:: Virginia Polytechnic Institute and State University;.
    [Google Scholar]
  14. Juvonen R. , Suihko M.-L. . ( 2006; ). Megasphaera paucivorans sp. nov., Megasphaera sueciensis sp. nov. and Pectinatus haikarae sp. nov., isolated from brewery samples, and emended description of the genus Pectinatus . . Int J Syst Evol Microbiol 56:, 695–702. [CrossRef] [PubMed]
    [Google Scholar]
  15. Juvonen R. , Satokari R. , Mallison K. , Haikara A. . ( 1999; ). Detection of spoilage bacteria in beer by PCR. . J. Am. Soc. Brew. Chem 57:, 99–103.
    [Google Scholar]
  16. Kim J. , Breidt F. . ( 2007; ). Development of preservation prediction chart for long term storage of fermented cucumber. . Korean J Life Sci 17:, 1616–1621. [CrossRef]
    [Google Scholar]
  17. Lee S. Y. . ( 1994; ). SMMP: A medium for selective isolation of Megasphaera and Pectinatus from the brewery. . J Am Soc Brew Chem 52:, 115–119.
    [Google Scholar]
  18. Lee S. Y. , Mabee M. S. , Jangaard N. O. . ( 1978; ). Pectinatus, a new genus of the family Bacteroidaceae . . Int J Syst Bacteriol 28:, 582–594. [CrossRef]
    [Google Scholar]
  19. Marchandin H. , Teyssier C. , Campos J. , Jean-Pierre H. , Roger F. , Gay B. , Carlier J.-P. , Jumas-Bilak E. . ( 2010; ). Negativicoccus succinicivorans gen. nov., sp. nov., isolated from human clinical samples, emended description of the family Veillonellaceae and description of Negativicutes classis nov., Selenomonadales ord. nov. and Acidaminococcaceae fam. nov. in the bacterial phylum Firmicutes . . Int J Syst Evol Microbiol 60:, 1271–1279. [CrossRef] [PubMed]
    [Google Scholar]
  20. Nocker A. , Camper A. K. . ( 2006; ). Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide. . Appl Environ Microbiol 72:, 1997–2004. [CrossRef] [PubMed]
    [Google Scholar]
  21. Pan Y. , Breidt F. Jr . ( 2007; ). Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells. . Appl Environ Microbiol 73:, 8028–8031. [CrossRef] [PubMed]
    [Google Scholar]
  22. Schleifer K. H. , Leuteritz M. , Weiss N. , Ludwig W. , Kirchhof G. , Seidel-Rüfer H. . ( 1990; ). Taxonomic study of anaerobic, Gram-negative, rod-shaped bacteria from breweries: emended description of Pectinatus cerevisiiphilus and description of Pectinatus frisingensis sp. nov., Selenomonas lacticifex sp. nov., Zymophilus raffinosivorans gen. nov., sp. nov., and Zymophilus paucivorans sp. nov.. Int J Syst Bacteriol 40:, 19–27. [CrossRef] [PubMed]
    [Google Scholar]
  23. Stackebrandt E. , Liesack W. . ( 1993; ). Nucleic acids and classification. . In Handbook of new bacterial systematics, pp. 152–189. Edited by Goodfellow M. , O'Donnell A. G. . London:: Academic Press;.
    [Google Scholar]
  24. Vereecke C. , Arahal D. R. . ( 2008; ). The status of the species Pectinatus portalensis Gonzalez et al. 2005. Request for an Opinion. . Int J Syst Evol Microbiol 58:, 1507. [CrossRef] [PubMed]
    [Google Scholar]
  25. Zhang W. W. , Fang M. X. , Tan H. Q. , Zhang X. Q. , Wu M. , Zhu X. F. . ( 2012; ). Pectinatus brassicae sp. nov., a Gram-negative, anaerobic bacterium isolated from salty wastewater. . Int J Syst Evol Microbiol 62:, 2145–2149. [CrossRef] [PubMed]
    [Google Scholar]
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