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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_7

gen. nov., sp. nov., a member of the family isolated from activated sludge Free

Abstract

A novel Gram-negative, non-spore-forming, rod-shaped strain, H1, was isolated from activated sludge by micromanipulation. No close relatives among cultured bacterial isolates were found; phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H1 forms a deep single branch in the family . Cells of strain H1 were slightly curved to straight rods (1.2–1.4×1.5–1.7 µm) and motile by a single polar flagellum. Strain H1 was able to grow in the presence of 0–4 % NaCl and grew optimally at 37 °C and pH 6.0–7.0. Chemotaxonomic analysis revealed that strain H1 possessed Q-10 as the predominant ubiquinone and Cω7, C and C cyclo ω8 as the major fatty acids. The DNA G+C content of strain H1 was 65.1 mol%. Comparative analysis of 16S rRNA gene sequences, and phenotypic and chemotaxonomic data, indicate that strain H1 should represent a novel genus and species of the family . The name gen. nov., sp. nov. is proposed. The type strain of is H1 ( = CICC 10529  = CCTCC AB 2012861  = KACC 16940).

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Funding
This study was supported by the:
  • Fund for Distinguished Young Scientist of Jiangsu Province (Award BK2012029)
  • National Key Technology R&D Program (Award 2012BAD14B02)
  • ‘948’ project of Ministry of Agriculture (Award 2011-Z21)
  • Fundamental Research Funds for the Central Universities (Award KYZ200920)
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2013-07-01
2023-09-24
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References

  1. Biebl H., Allgaier M., Tindall B. J., Koblizek M., Lünsdorf H., Pukall R., Wagner-Döbler I. ( 2005 ). Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates. . Int J Syst Evol Microbiol 55, 1089–1096. [View Article] [PubMed]
    [Google Scholar]
  2. Díaz-Cárdenas C., Patel B. K. C., Baena S. ( 2010 ). Tistlia consotensis gen. nov., sp. nov., an aerobic, chemoheterotrophic, free-living, nitrogen-fixing alphaproteobacterium, isolated from a Colombian saline spring. . Int J Syst Evol Microbiol 60, 1437–1443. [View Article] [PubMed]
    [Google Scholar]
  3. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  5. Imhoff J. F., Petri R., Süling J. ( 1998 ). Reclassification of species of the spiral-shaped phototrophic purple non-sulfur bacteria of the α-Proteobacteria: description of the new genera Phaeospirillum gen. nov., Rhodovibrio gen. nov., Rhodothalassium gen. nov. and Roseospira gen. nov. as well as transfer of Rhodospirillum fulvum to Phaeospirillum fulvum comb. nov., of Rhodospirillum molischianum to Phaeospirillum molischianum comb. nov., of Rhodospirillum salinarum to Rhodovibrio salinarum comb. nov., of Rhodospirillum sodomense to Rhodovibrio sodomensis comb. nov., of Rhodospirillum salexigens to Rhodothalassium salexigens comb. nov, and of Rhodospirillum mediosalinum to Roseospira mediosalina comb. nov.. Int J Syst Bacteriol 48, 793–798. [View Article] [PubMed]
    [Google Scholar]
  6. Kawasaki H., Hoshino Y., Kuraishi H., Yamasato K. ( 1992 ). Rhodocista centenaria gen. nov, sp. nov., a cyst-forming anoxygenic photosynthetic bacterium and its phylogenetic position in the Proteobacteria alpha-group. . J Gen Appl Microbiol 38, 541–551. [View Article]
    [Google Scholar]
  7. Lai Q., Yuan J., Wu C., Shao Z. ( 2009 ). Oceanibaculum indicum gen. nov., sp. nov., isolated from deep seawater of the Indian Ocean. . Int J Syst Evol Microbiol 59, 1733–1737. [View Article] [PubMed]
    [Google Scholar]
  8. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
     [Google Scholar]
  9. Liu Y., Jin J.-H., Liu Y.-H., Zhou Y.-G., Liu Z.-P. ( 2010 ). Dongia mobilis gen. nov., sp. nov., a new member of the family Rhodospirillaceae isolated from a sequencing batch reactor for treatment of malachite green effluent. . Int J Syst Evol Microbiol 60, 2780–2785. [View Article] [PubMed]
    [Google Scholar]
  10. Liu M., Dai J., Liu Y., Cai F., Wang Y., Rahman E., Fang C. ( 2011 ). Desertibacter roseus gen. nov., sp. nov., a gamma radiation-resistant bacterium in the family Rhodospirillaceae, isolated from desert sand. . Int J Syst Evol Microbiol 61, 1109–1113. [View Article] [PubMed]
    [Google Scholar]
  11. López-López A., Pujalte M. J., Benlloch S., Mata-Roig M., Rosselló-Mora R., Garay E., Rodríguez-Valera F. ( 2002 ). Thalassospira lucentensis gen. nov., sp. nov., a new marine member of the α-Proteobacteria . . Int J Syst Evol Microbiol 52, 1277–1283. [View Article] [PubMed]
    [Google Scholar]
  12. Mandel M., Marmur J. ( 1968 ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B, 195–206. [View Article]
    [Google Scholar]
  13. Maszenan A. M., Seviour R. J., Patel B. K. C., Janssen P. H., Wanner J. ( 2005 ). Defluvicoccus vanus gen. nov., sp. nov., a novel Gram-negative coccus/coccobacillus in the ‘Alphaproteobacteria’ from activated sludge. . Int J Syst Evol Microbiol 55, 2105–2111. [View Article] [PubMed]
    [Google Scholar]
  14. Mccarthy A. J., Cross T. ( 1984 ). A taxonomic study of Thermomonospora and other monosporic actinomycetes. . J Gen Microbiol 130, 5–25.
     [Google Scholar]
  15. Pfennig N., Lünsdorf H., Süling J., Imhoff J. F. ( 1997 ). Rhodospira trueperi gen. nov., spec. nov., a new phototrophic Proteobacterium of the alpha group. . Arch Microbiol 168, 39–45. [View Article] [PubMed]
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425. [PubMed]
    [Google Scholar]
  17. Schleifer K. H., Schuler D., Spring S., Weizenegger M., Amann R., Ludwig W., Kohler M. ( 1991 ). The genus Magnetospirillum gen. nov., description of Magnetospirillum gryphiswaldense sp, nov. and transfer of Aquaspirillum magnetotacticum to Magnetospirillum magnetotacticum comb. nov.. Syst Appl Microbiol 14, 379–385. [View Article]
    [Google Scholar]
  18. Sly L. I., Stackebrandt E. ( 1999 ). Description of Skermanella parooensis gen. nov., sp. nov. to accommodate Conglomeromonas largomobilis subsp. parooensis following the transfer of Conglomeromonas largomobilis subsp. largomobilis to the genus Azospirillum . . Int J Syst Bacteriol 49, 541–544. [View Article]
    [Google Scholar]
  19. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  20. Tarrand J. J., Krieg N. R., Döbereiner J. ( 1978 ). A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov.. Can J Microbiol 24, 967–980. [View Article] [PubMed]
    [Google Scholar]
  21. Urios L., Michotey V., Intertaglia L., Lesongeur F., Lebaron P. ( 2008 ). Nisaea denitrificans gen. nov., sp. nov. and Nisaea nitritireducens sp. nov., two novel members of the class Alphaproteobacteria from the Mediterranean Sea. . Int J Syst Evol Microbiol 58, 2336–2341. [View Article] [PubMed]
    [Google Scholar]
  22. Xie C. H., Yokota A. ( 2005 ). Pleomorphomonas oryzae gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from paddy soil of Oryza sativa . . Int J Syst Evol Microbiol 55, 1233–1237. [View Article] [PubMed]
    [Google Scholar]
  23. Yoon J. H., Kim H., Kim S. B., Kim H. J., Kim W. Y., Lee S. T., Goodfellow M., Park Y. H. ( 1996 ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46, 502–505. [View Article]
    [Google Scholar]
  24. Yoon J.-H., Kang S.-J., Park S., Oh T.-K. ( 2007 ). Caenispirillum bisanense gen. nov., sp. nov., isolated from sludge of a dye works. . Int J Syst Evol Microbiol 57, 1217–1221. [View Article] [PubMed]
    [Google Scholar]
  25. Zhang D. M., Yang H. F., Zhang W., Huang Z. Y., Liu S. J. ( 2003 ). Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. . Int J Syst Evol Microbiol 53, 1111–1114. [View Article] [PubMed]
    [Google Scholar]
  26. Zhang G. I., Hwang C. Y., Cho B. C. ( 2008 ). Thalassobaculum litoreum gen. nov., sp. nov., a member of the family Rhodospirillaceae isolated from coastal seawater. . Int J Syst Evol Microbiol 58, 479–485. [View Article] [PubMed]
    [Google Scholar]
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Taonella mepensis gen. nov., sp. nov., a member of the family Rhodospirillaceae isolated from activated sludge
Int J Syst Evol Microbiol 63, 2472 (2013); https://doi.org/10.1099/ijs.0.047803-0
/content/journal/ijsem/10.1099/ijs.0.047803-0
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