1887

Abstract

A novel Gram-negative, non-spore-forming, rod-shaped strain, H1, was isolated from activated sludge by micromanipulation. No close relatives among cultured bacterial isolates were found; phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H1 forms a deep single branch in the family . Cells of strain H1 were slightly curved to straight rods (1.2–1.4×1.5–1.7 µm) and motile by a single polar flagellum. Strain H1 was able to grow in the presence of 0–4 % NaCl and grew optimally at 37 °C and pH 6.0–7.0. Chemotaxonomic analysis revealed that strain H1 possessed Q-10 as the predominant ubiquinone and Cω7, C and C cyclo ω8 as the major fatty acids. The DNA G+C content of strain H1 was 65.1 mol%. Comparative analysis of 16S rRNA gene sequences, and phenotypic and chemotaxonomic data, indicate that strain H1 should represent a novel genus and species of the family . The name gen. nov., sp. nov. is proposed. The type strain of is H1 ( = CICC 10529  = CCTCC AB 2012861  = KACC 16940).

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2013-07-01
2020-01-28
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