1887

Abstract

A Gram-stain-positive, aerobic, spore-forming, rod-shaped bacterium, PN2, was isolated from a soil sample collected near the Pindari glacier. It contained anteiso-C, iso-C and Cω7 alcohol as the predominant fatty acids, MK-7 as the major menaquinone and A4α type (-Lys–-Glu) peptidoglycan. Based on these characteristics, strain PN2 was assigned to the genus Phylogenetic analysis based on 16S rRNA gene sequence placed strain PN2 within the genus and showed a sequence similarity of 98.5–99.0 % with members of this genus. CMS 21w, SK 55 and N-05 were identified as the most closely related species with 16S rRNA gene sequence similarities of 98.6 %, 99.0 % and 98.4 %, respectively. The values for DNA–DNA relatedness between strain PN2 and and were below the 70 % threshold value (32.0 %, 42.0 % and 38.0 % respectively). In addition, strain PN2 exhibited a number of phenotypic differences from , and Based on the cumulative differences, strain PN2 was identified as representing a novel species and the name sp. nov. was proposed. The type strain of sp. nov. is PN2 (LMG 23933 = JCM 15114). Furthermore, based on the morphological and chemotaxonomic characteristics, the species was reclassified as a species of the genus and renamed comb. nov In addition, an emended description of the genus is presented.

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2013-08-01
2019-12-14
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