1887

Abstract

A Gram-stain-positive, aerobic, spore-forming, rod-shaped bacterium, PN2, was isolated from a soil sample collected near the Pindari glacier. It contained anteiso-C, iso-C and Cω7 alcohol as the predominant fatty acids, MK-7 as the major menaquinone and A4α type (-Lys–-Glu) peptidoglycan. Based on these characteristics, strain PN2 was assigned to the genus Phylogenetic analysis based on 16S rRNA gene sequence placed strain PN2 within the genus and showed a sequence similarity of 98.5–99.0 % with members of this genus. CMS 21w, SK 55 and N-05 were identified as the most closely related species with 16S rRNA gene sequence similarities of 98.6 %, 99.0 % and 98.4 %, respectively. The values for DNA–DNA relatedness between strain PN2 and and were below the 70 % threshold value (32.0 %, 42.0 % and 38.0 % respectively). In addition, strain PN2 exhibited a number of phenotypic differences from , and Based on the cumulative differences, strain PN2 was identified as representing a novel species and the name sp. nov. was proposed. The type strain of sp. nov. is PN2 (LMG 23933 = JCM 15114). Furthermore, based on the morphological and chemotaxonomic characteristics, the species was reclassified as a species of the genus and renamed comb. nov In addition, an emended description of the genus is presented.

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2013-08-01
2020-10-19
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403410.[PubMed] [CrossRef]
    [Google Scholar]
  2. An S. Y., Haga T., Kasai H., Goto K., Yokota A. ( 2007 ). Sporosarcina saromensis sp. nov., an aerobic endospore-forming bacterium. . Int J Syst Evol Microbiol 57, 18681871.[PubMed] [CrossRef]
    [Google Scholar]
  3. Chaturvedi P., Shivaji S. ( 2006 ). Exiguobacterium indicum sp. nov., a psychrophilic bacterium from the Hamta glacier of the Himalayan mountain ranges of India. . Int J Syst Evol Microbiol 56, 27652770. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chaturvedi P., Reddy G. S. N., Shivaji S. ( 2005 ). Dyadobacter hamtensis sp. nov., from Hamta glacier, located in the Himalayas, India. . Int J Syst Evol Microbiol 55, 21132117. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 22592261. [CrossRef] [PubMed]
    [Google Scholar]
  6. Ghosh A., Bhardwaj M., Satyanarayana T., Khurana M., Mayilraj S., Jain R. K. ( 2007 ). Bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil. . Int J Syst Evol Microbiol 57, 238242. [CrossRef] [PubMed]
    [Google Scholar]
  7. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kishore K. H., Begum Z., Pathan A. A., Shivaji S. ( 2010 ). Paenibacillus glacialis sp. nov., isolated from the Kafni glacier of the Himalayas, India. . Int J Syst Evol Microbiol 60, 19091913. [CrossRef] [PubMed]
    [Google Scholar]
  9. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [CrossRef]
    [Google Scholar]
  10. Krishnamurthi S., Bhattacharya A., Mayilraj S., Saha P., Schumann P., Chakrabarti T. ( 2009 ). Description of Paenisporosarcina quisquiliarum gen. nov., sp. nov., and reclassification of Sporosarcina macmurdoensis Reddy et al. 2003 as Paenisporosarcina macmurdoensis comb. nov.. Int J Syst Evol Microbiol 59, 13641370. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kwon S. W., Kim B. Y., Song J., Weon H. Y., Schumann P., Tindall B. J., Stackebrandt E., Fritze D. ( 2007 ). Sporosarcina koreensis sp. nov. and Sporosarcina soli sp. nov., isolated from soil in Korea. . Int J Syst Evol Microbiol 57, 16941698. [CrossRef] [PubMed]
    [Google Scholar]
  12. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [CrossRef]
    [Google Scholar]
  13. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. & other authors ( 2009 ). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59, 21142121. [CrossRef] [PubMed]
    [Google Scholar]
  14. Mayilraj S., Prasad G. S., Suresh K., Saini H. S., Shivaji S., Chakrabarti T. ( 2005 ). Planococcus stackebrandtii sp. nov., isolated from a cold desert of the Himalayas, India. . Int J Syst Evol Microbiol 55, 9194. [CrossRef] [PubMed]
    [Google Scholar]
  15. Mayilraj S., Kroppenstedt R. M., Suresh K., Saini H. S. ( 2006a ). Kocuria himachalensis sp. nov., an actinobacterium isolated from the Indian Himalayas. . Int J Syst Evol Microbiol 56, 19711975. [CrossRef] [PubMed]
    [Google Scholar]
  16. Mayilraj S., Suresh K., Schumann P., Kroppenstedt R. M., Saini H. S. ( 2006b ). Agrococcus lahaulensis sp. nov., isolated from a cold desert of the Indian Himalayas. . Int J Syst Evol Microbiol 56, 18071810. [CrossRef] [PubMed]
    [Google Scholar]
  17. Mayilraj S., Suresh K., Kroppenstedt R. M., Saini H. S. ( 2006c ). Dietzia kunjamensis sp. nov., isolated from the Indian Himalayas. . Int J Syst Evol Microbiol 56, 16671671. [CrossRef] [PubMed]
    [Google Scholar]
  18. Mayilraj S., Saha P., Suresh K., Saini H. S. ( 2006d ). Ornithinimicrobium kibberense sp. nov., isolated from the Indian Himalayas. . Int J Syst Evol Microbiol 56, 16571661. [CrossRef] [PubMed]
    [Google Scholar]
  19. Mayilraj S., Krishnamurthi S., Saha P., Saini H. S. ( 2006e ). Rhodococcus kroppenstedtii sp. nov., a novel actinobacterium isolated from a cold desert of the Himalayas, India. . Int J Syst Evol Microbiol 56, 979982. [CrossRef] [PubMed]
    [Google Scholar]
  20. Pandey K. K., Mayilraj S., Chakrabarti T. ( 2002 ). Pseudomonas indica sp. nov., a novel butane-utilizing species. . Int J Syst Evol Microbiol 52, 15591567. [CrossRef] [PubMed]
    [Google Scholar]
  21. Pindi P. K., Kishore K. H., Reddy G. S. N., Shivaji S. ( 2009 ). Description of Leifsonia kafniensis sp. nov. and Leifsonia antarctica sp. nov.. Int J Syst Evol Microbiol 59, 13481352. [CrossRef] [PubMed]
    [Google Scholar]
  22. Pradhan S., Srinivas T. N. R., Pindi P. K., Kishore K. H., Begum Z., Singh P. K., Singh A. K., Pratibha M. S., Yasala A. K. & other authors ( 2010 ). Bacterial biodiversity from Roopkund Glacier, Himalayan mountain ranges, India. . Extremophiles 14, 377395. [CrossRef] [PubMed]
    [Google Scholar]
  23. Reddy G. S. N., Aggarwal R. K., Matsumoto G. I., Shivaji S. ( 2000 ). Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. . Int J Syst Evol Microbiol 50, 15531561. [CrossRef] [PubMed]
    [Google Scholar]
  24. Reddy G. S. N., Prakash J. S. S., Vairamani M., Prabhakar S., Matsumoto G. I., Shivaji S. ( 2002 ). Planococcus antarcticus and Planococcus psychrophilus spp. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. . Extremophiles 6, 253261. [CrossRef] [PubMed]
    [Google Scholar]
  25. Reddy G. S. N., Matsumoto G. I., Shivaji S. ( 2003 ). Sporosarcina macmurdoensis sp. nov., from a cyanobacterial mat sample from a pond in the McMurdo Dry Valleys, Antarctica. . Int J Syst Evol Microbiol 53, 13631367. [CrossRef] [PubMed]
    [Google Scholar]
  26. Reddy G. S. N., Uttam A., Shivaji S. ( 2008a ). Bacillus cecembensis sp. nov. from the Pindari glacier of the Indian Himalayas. . Int J Syst Evol Microbiol 58, 23302335. [CrossRef] [PubMed]
    [Google Scholar]
  27. Reddy G. S. N., Prabagaran S. R., Shivaji S. ( 2008b ). Leifsonia pindariensis sp. nov., isolated from the Pindari glacier of the Indian Himalayas and emended description of the genus Leifsonia . . Int J Syst Evol Microbiol 58, 22292234. [CrossRef] [PubMed]
    [Google Scholar]
  28. Reddy G. S. N., Pradhan S., Manorama R., Shivaji S. ( 2010 ). Cryobacterium roopkundense sp. nov., a psychrophilic bacterium isolated from glacial soil. . Int J Syst Evol Microbiol 60, 866870. [CrossRef] [PubMed]
    [Google Scholar]
  29. Shivaji S., Chaturvedi P., Reddy G. S., Suresh K. ( 2005 ). Pedobacter himalayensis sp. nov., from the Hamta glacier located in the Himalayan mountain ranges of India. . Int J Syst Evol Microbiol 55, 10831088. [CrossRef] [PubMed]
    [Google Scholar]
  30. Shivaji S., Pratibha M. S., Sailaja B., Hara Kishore K., Singh A. K., Begum Z., Anarasi U., Prabagaran S. R., Reddy G. S. N., Srinivas T. N. R. ( 2011 ). Bacterial diversity of soil in the vicinity of Pindari glacier, Himalayan mountain ranges, India, using culturable bacteria and soil 16S rRNA gene clones. . Extremophiles 15, 122. [CrossRef] [PubMed]
    [Google Scholar]
  31. Singla A. K., Mayilraj S., Kudo T., Krishnamurthi S., Prasad G. S., Vohra R. M. ( 2005 ). Actinoalloteichus spitiensis sp. nov., a novel actinobacterium isolated from a cold desert of the Indian Himalayas. . Int J Syst Evol Microbiol 55, 25612564. [CrossRef] [PubMed]
    [Google Scholar]
  32. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  33. Srinivas T. N., Singh S. M., Pradhan S., Pratibha M. S., Kishore K. H., Singh A. K., Begum Z., Prabagaran S. R., Reddy G. S., Shivaji S. ( 2011 ). Comparison of bacterial diversity in proglacial soil from Kafni Glacier, Himalayan Mountain ranges, India, with the bacterial diversity of other glaciers in the world. . Extremophiles 15, 673690. [CrossRef] [PubMed]
    [Google Scholar]
  34. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [CrossRef]
    [Google Scholar]
  35. Tamaoka J. ( 1986 ). Analysis of bacterial menaquinone mixtures by reverse-phase high-performance liquid chromatography. . Methods Enzymol 123, 251256. [CrossRef] [PubMed]
    [Google Scholar]
  36. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  37. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  38. Tominaga T., An S. Y., Oyaizu H., Yokota A. ( 2009 ). Sporosarcina luteola sp. nov. isolated from soy sauce production equipment in Japan. . J Gen Appl Microbiol 55, 217223. [CrossRef] [PubMed]
    [Google Scholar]
  39. Yu Y., Xin Y. H., Liu H. C., Chen B., Sheng J., Chi Z. M., Zhou P. J., Zhang D. C. ( 2008 ). Sporosarcina antarctica sp. nov., a psychrophilic bacterium isolated from the Antarctic. . Int J Syst Evol Microbiol 58, 21142117. [CrossRef] [PubMed]
    [Google Scholar]
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