1887

Abstract

Two novel siderophore-producing strains designated CC-CFT023 and CC-CFT034, isolated from a rusty iron plate, are characterized in this study. Cells of both strains were catalase- and oxidase-positive. Strain CC-CFT023 showed a high pairwise 16S rRNA gene sequence similarity to LMG 23375 (96.0 %), whereas, strain CC-CFT034 shared a maximum similarity to DSM 18598 (95.8 %). The predominant quinone system was ubiquinone (Q-10) and the DNA G+C contents of CC-CFT023 and CC-CFT034 were 69.5 mol% and 67.7 mol%, respectively. The major fatty acids of these strains were C, C, C cyclo ω8, C 2-OH and summed features 3 and 8, consisting of Cω7/Cω6 and Cω7/Cω6, respectively. Polar lipid profiles of both of these isolates include diphosphatidylglycerol (DPG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and most importantly sulfoquinovosyldiacylglycerol (SQDG), which is one of the typical molecular features reported to delineate the members of the genus from their closest relatives, species of the genus . Based on the phylogenetic, phenotypic and chemotaxonomic features, strains CC-CFT023 and CC-CFT034 represent two novel species within the genus , for which the names sp. nov. (type strain CC-CFT023 = BCRC 80439 = JCM 18444) and sp. nov. (type strain CC-CFT034 = BCRC 80440 = JCM 18445) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.047431-0
2013-07-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/7/2430.html?itemId=/content/journal/ijsem/10.1099/ijs.0.047431-0&mimeType=html&fmt=ahah

References

  1. Anderson C. R. , Dick G. J. , Chu M.-L. , Cho J.-C. , Davis R. E. , Bräuer S. L. , Tebo B. M. . ( 2009; ). Aurantimonas manganoxydans, sp. nov. and Aurantimonas litoralis, sp. nov.: Mn(II) oxidizing representatives of a globally distributed clade of alpha-Proteobacteria from the order Rhizobiales . . Geomicrobiol J 26:, 189–198. [CrossRef] [PubMed]
    [Google Scholar]
  2. Chaiharn M. , Chunhaleuchanon S. , Lumyong S. . ( 2009; ). Screening siderophore producing bacteria as potential biological control agent for fungal rice pathogens in Thailand. . World J Microbiol Biotechnol 25:, 1919–1928. [CrossRef]
    [Google Scholar]
  3. Collins M. D. . ( 1985; ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  4. Denner E. B. M. , Smith G. W. , Busse H.-J. , Schumann P. , Narzt T. , Polson S. W. , Lubitz W. , Richardson L. L. . ( 2003; ). Aurantimonas coralicida gen. nov., sp. nov., the causative agent of white plague type II on Caribbean scleractinian corals. . Int J Syst Evol Microbiol 53:, 1115–1122. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. GCG ( 1995; ). Wisconsin Package Version 8.1 Program Manual. Madison, WI:: Genetics Computer Group;.
    [Google Scholar]
  7. Guerinot M. L. . ( 1994; ). Microbial iron transport. . Annu Rev Microbiol 48:, 743–772. [CrossRef] [PubMed]
    [Google Scholar]
  8. Heiner C. R. , Hunkapiller K. L. , Chen S. M. , Glass J. I. , Chen E. Y. . ( 1998; ). Sequencing multimegabase-template DNA with BigDye terminator chemistry. . Genome Res 8:, 557–561.[PubMed]
    [Google Scholar]
  9. Hung M.-H. , Bhagwath A. A. , Shen F.-T. , Devasya R. P. , Young C.-C. . ( 2005; ). Indigenous rhizobia associated with native shrubby legumes in Taiwan. . Pedobiologia (Jena) 49:, 577–584. [CrossRef]
    [Google Scholar]
  10. Jurado V. , Gonzalez J. M. , Laiz L. , Saiz-Jimenez C. . ( 2006; ). Aurantimonas altamirensis sp. nov., a member of the order Rhizobiales isolated from Altamira Cave. . Int J Syst Evol Microbiol 56:, 2583–2585. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kim M. S. , Hoa K. T. Q. , Baik K. S. , Park S. C. , Seong C. N. . ( 2008; ). Aurantimonas frigidaquae sp. nov., isolated from a water-cooling system. . Int J Syst Evol Microbiol 58:, 1142–1146. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  13. Maidak B. L. , Cole J. R. , Lilburn T. G. , Parker C. T. Jr , Saxman P. R. , Farris R. J. , Garrity G. M. , Olsen G. J. , Schmidt T. M. , Tiedje J. M. . ( 2001; ). The RDP-II (Ribosomal Database Project). . Nucleic Acids Res 29:, 173–174. [CrossRef] [PubMed]
    [Google Scholar]
  14. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  15. Miller L. T. . ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  16. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal K. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  17. Murray R. G. E. , Doetsch R. N. , Robinow F. . ( 1994; ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  18. Neilands J. B. . ( 1995; ). Siderophores: structure and function of microbial iron transport compounds. . J Biol Chem 270:, 26723–26726.[PubMed] [CrossRef]
    [Google Scholar]
  19. Paisley R. . ( 1996; ). MIS Whole Cell Fatty Acid Analysis by Gas Chromatography Training Manual. Newark, DE:: MIDI;.
    [Google Scholar]
  20. Rathsack K. , Reitner J. , Stackebrandt E. , Tindall B. J. . ( 2011; ). Reclassification of Aurantimonas altamirensis (Jurado et al. 2006), Aurantimonas ureilytica (Weon et al. 2007) and Aurantimonas frigidaquae (Kim et al. 2008) as members of a new genus, Aureimonas gen. nov., as Aureimonas altamirensis gen. nov., comb. nov., Aureimonas ureilytica comb. nov. and Aureimonas frigidaquae comb. nov., and emended descriptions of the genera Aurantimonas and Fulvimarina . . Int J Syst Evol Microbiol 61:, 2722–2728. [CrossRef] [PubMed]
    [Google Scholar]
  21. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  22. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids . , MIDI Technical Note 101. Newark, DE:: MIDI Inc.;
  23. Schwyn B. , Neilands J. B. . ( 1987; ). Universal chemical assay for the detection and determination of siderophores. . Anal Biochem 160:, 47–56. [CrossRef] [PubMed]
    [Google Scholar]
  24. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characteristics. . In Methods for General and Molecular Biology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  25. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  26. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  27. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  28. Tortora M. L. , Díaz-Ricci J. C. , Pedraza R. O. . ( 2011; ). Azospirillum brasilense siderophores with antifungal activity against Colletotrichum acutatum . . Arch Microbiol 193:, 275–286. [CrossRef] [PubMed]
    [Google Scholar]
  29. Watts D. , MacBeath J. R. . ( 2001; ). Automated fluorescent DNA sequencing on the ABI PRISM 310 Genetic Analyzer. . Methods Mol Biol 167:, 153–170.[PubMed]
    [Google Scholar]
  30. Weon H.-Y. , Kim B.-Y. , Yoo S.-H. , Joa J.-H. , Lee K. H. , Zhang Y.-S. , Kwon S.-W. , Koo B.-S. . ( 2007; ). Aurantimonas ureilytica sp. nov., isolated from an air sample. . Int J Syst Evol Microbiol 57:, 1717–1720. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.047431-0
Loading
/content/journal/ijsem/10.1099/ijs.0.047431-0
Loading

Data & Media loading...

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error