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Volume 63, Issue Pt_8

sp. nov., a moderately halophilic bacterium from a hypersaline lake Free

Abstract

A novel Gram-stain-positive, moderately halophilic bacterium, designated strain E33, was isolated from water of the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain E33 were motile rods and produced ellipsoidal endospores at a central or subterminal position in swollen sporangia. Strain E33 was a strictly aerobic bacterium, catalase- and oxidase-positive. The strain was able to grow at NaCl concentrations of 0.5–25 % (w/v), with optimum growth occurring at 5–15 % (w/v) NaCl. The optimum temperature and pH for growth were 40 °C and pH 7.5–8.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain E33 was shown to belong to the genus within the phylum and showed the closest phylogenetic similarity with the species 4T19 (99.2 %), D-1-5a (97.3 %) and SW-211 (97.2 %). The DNA G+C content of the type strain of the novel species was 42.6 mol%. The major cellular fatty acids of strain E33 were anteiso-C and iso-C, and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids, an unknown lipid and an unknown phospholipid. The isoprenoid quinones were MK-7 (97 %), MK-6 (2 %) and MK-8 (0.5 %). The peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. All these features confirm the placement of isolate E33 within the genus . DNA–DNA hybridization experiments revealed low levels of relatedness between strain E33 and IBRC-M 10590 (22 %), CCM 7228 (38 %) and DSM 16303 (19 %). On the basis of polyphasic evidence from this study, a novel species of the genus , sp. nov. is proposed, with strain E33 ( = IBRC-M 10095 = DSM 25387) as the type strain.

  • Published Online:
Funding
This study was supported by the:
  • Iranian Biological Resource Centre (IBRC) (Award MI-1388-04)
  • International Foundation for Science (IFS) (Award A/4527-1)
  • Spanish Ministerio de Economía y Competitividad (Award CGL2010-19303)
  • European Regional Development Fund (FEDER)
  • National Science Foundation (Award Grant DEB-0919290)
  • Junta de Andalucía (Award P10-CVI-6226)
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2013-08-01
2024-12-08
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Bacillushalosaccharovorans sp. nov., a moderately halophilic bacterium from a hypersaline lake
Int J Syst Evol Microbiol 63, 2776 (2013); https://doi.org/10.1099/ijs.0.046961-0
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