1887

Abstract

A novel Gram-positive, multiloculated thalli-forming, aerobic, actinobacterial strain, CF9/1/1, was isolated in 2007 during environmental screening for xerophilic fungi in arid desert soil from the Sahara desert, Chad. The isolate grew best at a temperature range of 20–35 °C and at pH 6.0–8.5 and with 0–4 % (w/v) NaCl, forming black-coloured and irregular colonies on GYM agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus . The DNA G+C content of the novel strain was 75.4 mol%. The peptidoglycan contained -diaminopimelic acid as a diagnostic diamino acid. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, a not yet structurally identified aminophospholipid and a small amount of phosphatidylglycerol; MK-9(H) was identified as the dominant menaquinone and galactose was a diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids: iso-C and iso-C. The 16S rRNA gene sequence of the isolate showed 94.6–97.0 % sequence similarities with those of five members of the genus: DSM 45317 (94.6 %), DSM 43160 (94.8 %), DSM 45419 (96.2 %), DSM 45408 (96.7 %) and DSM 45418 (97.0 %). Based on the evidence from this polyphasic taxonomic study, a novel species, sp. nov., is proposed; the type strain is CF9/1/1 ( = DSM 45421 = CCUG 62764).

Funding
This study was supported by the:
  • , European Social Fund Operational Programme
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2013-06-01
2020-08-04
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