1887

Abstract

A Gram-negative, rod-shaped, aerobic bacterial strain, designated EBTL01, was isolated from activated sludge by using metabolites of microalgae SAG278-2. Phylogenetic analyses based on 16S rRNA gene sequence showed that strain EBTL01 belongs to the family , class , and is related most closely to A4T-83 (95.5 % sequence similarity) and A5J-41-2 (95.2 %). The G+C content of the genomic DNA of strain EBTL01 was 56.3 mol% and the menaquinone MK-9 was detected as the predominant quinone. Major fatty acid components were iso-C, Cω7 and C. The amino acids of the cell-wall peptidoglycan contained muramic acid and -diaminopimelic acid. These profile results supported the affiliation of strain EBTL01 to the genus . On the other hand, based on chemotaxonomic properties and phenotypic characteristics, strain EBTL01 could be clearly differentiated from its phylogenetic neighbours. Therefore, strain EBTL01 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EBTL01 ( = KCTC 23678 = JCM 18052).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.046664-0
2013-05-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/5/1891.html?itemId=/content/journal/ijsem/10.1099/ijs.0.046664-0&mimeType=html&fmt=ahah

References

  1. Cappuccino J. G. , Sherman N. . ( 2002; ). Microbiology: a Laboratory Manual, , 6th edn.. Menlo Park, CA:: Benjamin/Cummings;.
    [Google Scholar]
  2. Collins C. H. , Lyne P. M. . ( 1984; ). Microbiological Methods, , 5th edn.. London:: Butterworth;.
    [Google Scholar]
  3. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  4. Felsenstein J. . ( 1985; ). Confidence limit on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  5. Felsenstein J. . ( 2009; ). phylip (phylogeny inference package) version 3 . 57c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  6. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  7. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  8. Hiraishi A. , Ueda Y. , Ishihara J. , Mori T. . ( 1996; ). Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. . J Gen Appl Microbiol 42:, 457–469. [CrossRef]
    [Google Scholar]
  9. Kim M. K. , Im W.-T. , Ohta H. , Lee M. , Lee S.-T. . ( 2005; ). Sphingopyxis granuli sp. nov., a β-glucosidase-producing bacterium in the family Sphingomonadaceae in α-4 subclass of the Proteobacteria . . J Microbiol 43:, 152–157.[PubMed]
    [Google Scholar]
  10. Kim O.-S. , Cho Y.-J. , Lee K. , Yoon S.-H , Kim M. , Na H. , Park S.-C. , Jeon Y. S. , Lee J.-H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721.[CrossRef]
    [Google Scholar]
  11. Kimura M. . ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  12. Kuykendall L. D. , Roy M. A. , O'Neill J. J. , Devine T. E. . ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38:, 358–361.[CrossRef]
    [Google Scholar]
  13. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  14. Moore D. D. , Dowhan D. . ( 1995; ). Preparation and analysis of DNA. . In Current Protocols in Molecular Biology, pp. 2–11. Edited by Ausubel F. W. , Brent R. , Kingston R. E. , Moore D. D. , Seidman J. G. , Smith J. A. , Struhl K. . . New York:: Wiley;.
    [Google Scholar]
  15. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  16. Sasser M. . ( 1990; ). Identification of bacteria through fatty acid analysis. . In Methods in Phytobacteriology, pp. 199–204. Edited by Klement Z. , Rudolph K. , Sands D. C. . . Budapest:: Akademiai Kaido;.
    [Google Scholar]
  17. Schleifer K. H. . ( 1985; ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18:, 123–156. [CrossRef]
    [Google Scholar]
  18. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  19. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  20. Stackebrandt E. , Frederiksen W. , Garrity G. M. , Grimont P. A. D. , Kämpfer P. , Maiden M. C. , Nesme X. , Rosselló-Mora R. , Swings J. . & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52:, 1043–1047. [CrossRef] [PubMed]
    [Google Scholar]
  21. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4 . 0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  22. Ten L. N. , Im W.-T. , Kim M.-K. , Kang M.-S. , Lee S.-T. . ( 2004; ). Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. . J Microbiol Methods 56:, 375–382. [CrossRef] [PubMed]
    [Google Scholar]
  23. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  24. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  25. Yoon J. , Matsuo Y. , Adachi K. , Nozawa M. , Matsuda S. , Kasai H. , Yokota A. . ( 2008; ). Description of Persicirhabdus sediminis gen. nov., sp. nov., Roseibacillus ishigakijimensis gen. nov., sp. nov., Roseibacillus ponti sp. nov., Roseibacillus persicicus sp. nov., Luteolibacter pohnpeiensis gen. nov., sp. nov. and Luteolibacter algae sp. nov., six marine members of the phylum ‘Verrucomicrobia’, and emended descriptions of the class Verrucomicrobiae, the order Verrucomicrobiales and the family Verrucomicrobiaceae . . Int J Syst Evol Microbiol 58:, 998–1007. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.046664-0
Loading
/content/journal/ijsem/10.1099/ijs.0.046664-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error