1887

Abstract

A Gram-positive, aerobic, rod-shaped bacterial strain, 5116S-4, was isolated from an air sample collected in Suwon city, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain was a new member of the family . The sequence similarities of this strain to the members of the family were less than 97 %, with the highest values observed with MSL-15 (96.3 %), RuGl7 (96.2 %), KSW2-17 (96.2 %), Hh15 (96.2 %), DSM 4854 (96.2 %), 44C3 (96.0 %) and KSL51201-037 (96.0 %). According to the phylogenetic tree, strain 5116S-4 formed a cluster with KSL51201-037, KSW2-17 and SGM3-12 (95.3 % sequence similarity) on the support of high bootstrap values. Cells were motile with single polar flagellum and showed optimum growth at 30 °C and pH 7 without NaCl. Predominant cellular fatty acids were anteiso-C, iso-C and anteiso-C. Polar lipids were diphosphatidylglycerol, dimannosyldiacylglycerol, phosphatidylglycerol, one unknown glycolipid, one unknown phospholipid and one unknown lipid. The acyl type of the cell-wall muramic acid was the acetyl type. Peptidoglycan was supposed to be the type B1 with 2,4-diaminobutyric acid on position 3. Strain 5116S-4 was clearly distinguishable from the phylogenetically related genera in the family in terms of chemotaxonomic characteristics. On the basis of the chemotaxonomic and phylogenetic characteristics, a novel genus and species are proposed, gen. nov., sp. nov. The type strain of is 5116S-4 ( = KACC 15517  = NBRC 108725).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.046599-0
2013-07-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/7/2436.html?itemId=/content/journal/ijsem/10.1099/ijs.0.046599-0&mimeType=html&fmt=ahah

References

  1. Breznak J. A. , Costilow R. N. . ( 1994; ). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  2. Chun J. , Lee J. H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  3. Dastager S. G. , Lee J. C. , Ju Y. J. , Park D. J. , Kim C. J. . ( 2008; ). Cryobacterium mesophilum sp. nov., a novel mesophilic bacterium. . Int J Syst Evol Microbiol 58:, 1241–1244. [CrossRef] [PubMed]
    [Google Scholar]
  4. Groth I. , Schumann P. , Weiss N. , Martin K. , Rainey F. A. . ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46:, 234–239. [CrossRef] [PubMed]
    [Google Scholar]
  5. Hamada M. , Iino T. , Iwami T. , Harayama S. , Tamura T. , Suzuki K. . ( 2010; ). Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov.. J Gen Appl Microbiol 56:, 427–436. [CrossRef] [PubMed]
    [Google Scholar]
  6. Hiraishi A. . ( 1992; ). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. . Lett Appl Microbiol 15:, 210–213. [CrossRef] [PubMed]
    [Google Scholar]
  7. Jang Y. H. , Kim S. J. , Tamura T. , Hamada M. , Weon H. Y. , Suzuki K. I. , Kwon S. W. , Kim W. G. . ( 2013; ). Lysinimonas soli gen. nov., sp. nov., isolated from soil, and reclassification of Leifsonia kribbensis Dastager et al. 2009 as Lysinimonas kribbensis sp. nov., comb. nov. . Int J Syst Evol Microbiol 63:, 1403–1410. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kim S.-J. , Lee S.-S. . ( 2011; ). Amnibacterium kyonggiense gen. nov., sp. nov., a new member of the family Microbacteriaceae . . Int J Syst Evol Microbiol 61:, 155–159. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kluge A. G. , Farris F. S . ( 1969; ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32.[PubMed] [CrossRef]
    [Google Scholar]
  10. Kwon S. W. , Kim J. S. , Park I. C. , Yoon S. H. , Park D. H. , Lim C. K. , Go S. J. . ( 2003; ). Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. . Int J Syst Evol Microbiol 53:, 21–27. [CrossRef] [PubMed]
    [Google Scholar]
  11. Lee S. D. . ( 2007; ). Labedella gwakjiensis gen. nov., sp. nov., a novel actinomycete of the family Microbacteriaceae . . Int J Syst Evol Microbiol 57:, 2498–2502. [CrossRef] [PubMed]
    [Google Scholar]
  12. Ludwig W. , Strunk O. , Westram R. , Richter L. , Meier H. , Yadhukumar , Buchner A. , Lai T. , Steppi S. . & other authors ( 2004; ). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef] [PubMed]
    [Google Scholar]
  13. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  14. Minnikin D. E. , Hutchinson I. G. , Caldicott A. B. , Goodfellow M. . ( 1980; ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188:, 221–233. [CrossRef]
    [Google Scholar]
  15. Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  16. Nozawa Y. , Sakai N. , Arai K. , Kawasaki Y. , Harada K. . ( 2007; ). Reliable and sensitive analysis of amino acids in the peptidoglycan of actinomycetes using the advanced Marfey’s method. . J Microbiol Methods 70:, 306–311. [CrossRef] [PubMed]
    [Google Scholar]
  17. Pruesse E. , Quast C. , Knittel K. , Fuchs B. M. , Ludwig W. , Peplies J. , Glöckner F. O. . ( 2007; ). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb . . Nucleic Acids Res 35:, 7188–7196. [CrossRef] [PubMed]
    [Google Scholar]
  18. Reddy G. S. N. , Pradhan S. , Manorama R. , Shivaji S. . ( 2010; ). Cryobacterium roopkundense sp. nov., a psychrophilic bacterium isolated from glacial soil. . Int J Syst Evol Microbiol 60:, 866–870. [CrossRef] [PubMed]
    [Google Scholar]
  19. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  20. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  21. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  22. Stackebrandt E. , Rainey F. A. , Ward-Rainey N. L. . ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47:, 479–491. [CrossRef]
    [Google Scholar]
  23. Suzuki K. , Sasaki J. , Uramoto M. , Nakase T. , Komagata K. . ( 1997; ). Cryobacterium psychrophilum gen. nov., sp. nov., nom. rev., comb. nov., an obligately psychrophilic actinomycete to accommodate ‘Curtobacterium psychrophilum’ Inoue and Komagata 1976. . Int J Syst Bacteriol 47:, 474–478. [CrossRef] [PubMed]
    [Google Scholar]
  24. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  25. Uchida K. , Aida K. . ( 1984; ). An improved method for the glycolate test for simple identification of acyl type of bacterial cell walls. . J Gen Appl Microbiol 30:, 131–134. [CrossRef]
    [Google Scholar]
  26. Zgurskaya H. I. , Evtushenko L. I. , Akimov V. N. , Kalakoutskii L. V. . ( 1993; ). Rathayibacter gen. nov., including the species Rathayibacter rathayi comb. nov., Rathayibacter tritici comb. nov., Rathayibacter iranicus comb. nov., and six strains from annual grasses. . Int J Syst Bacteriol 43:, 143–149. [CrossRef]
    [Google Scholar]
  27. Zhang D.-C. , Wang H.-X. , Cui H.-L. , Yang Y. , Liu H.-C. , Dong X.-Z. , Zhou P.-J. . ( 2007; ). Cryobacterium psychrotolerans sp. nov., a novel psychrotolerant bacterium isolated from the China No. 1 glacier. . Int J Syst Evol Microbiol 57:, 866–869. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.046599-0
Loading
/content/journal/ijsem/10.1099/ijs.0.046599-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error