1887

Abstract

A Gram-stain-positive, spore-forming, rod-shaped, motile, strictly aerobic bacterial strain, designated CAU 1051, was isolated from a sand dune and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1051 grew optimally at pH 5.0 and 30 °C. NaCl was not required for growth but up to 10.0 % (w/v) NaCl was tolerated. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1051 formed a distinct lineage within the genus and was most closely related to CL-MP28, S-11, and LMG 19492 (96.8 %, 95.6 % and 95.3 % similarity, respectively). DNA–DNA reassociation analysis showed that strain CAU 1051 displayed 28.2±0.7 % relatedness to KCTC 13625. Strain CAU 1051 contained MK-7 as the only isoprenoid quinone and anteiso-C as the major fatty acid. The cell wall peptidoglycan of strain CAU 1051 contained -diaminopimelic acid. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, six unidentified phospholipids, an unidentified glycolipid, and six unidentified polar lipids. The major whole-cell sugars were glucose and ribose. The DNA G+C content was 36.3 mol%. On the basis of phenotypic data and phylogenetic inference, strain CAU 1051 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is CAU 1051 ( = KCTC 33035 = CCUG 63270).

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2013-10-01
2019-10-19
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