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Abstract

Two Gram-stain-negative, non-motile, non-pigmented cocci, designated IMCC11369 and IMCC11389, were isolated from surface seawater of the East Sea of Korea by high-throughput cultivation based on dilution to extinction. Strains IMCC11369 and IMCC11389 shared 99.9 % 16S rRNA gene sequence similarity and >86.3 % DNA–DNA relatedness, which suggested that they belong to the same genomic species. The isolates were most closely related to HTCC2155 (99.0 % 16S rRNA gene sequence similarity). Phylogenetic analysis revealed that the isolates formed a robust cluster with HTCC2155. DNA–DNA relatedness values, however, showed that the isolates were distantly related to HTCC2155 (2.0–18.6 %), which suggested that they represent a separate genomic species in the genus . The two isolates were phenotypically differentiated from their closest relative by several characteristics, including degradation of macromolecules and carbon source utilization. The DNA G+C content was 44.5–45.2 mol% and the predominant cellular fatty acids were C, Cω9 and C. Strain IMCC11369 contained MK-7 as the respiratory quinone and phosphatidylethanolamine and an unknown lipid as the major polar lipids. On the basis of data obtained in this study, a novel species is proposed to accommodate the isolates, sp. nov. The type strain is IMCC11369 ( = KCTC 23780 = NBRC 108776).

Funding
This study was supported by the:
  • National Marine Life Collection Program, Ministry of Land, Transport and Maritime Affairs, Korea (Award MABIK 2012-001-03)
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2013-04-01
2024-10-06
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References

  1. Cho J.-C., Giovannoni S. J. ( 2003 ). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria . . Int J Syst Evol Microbiol 53, 10311036. [View Article] [PubMed]
    [Google Scholar]
  2. Cho J.-C., Vergin K. L., Morris R. M., Giovannoni S. J. ( 2004 ). Lentisphaera araneosa gen. nov., sp. nov, a transparent exopolymer producing marine bacterium, and the description of a novel bacterial phylum, Lentisphaerae . . Environ Microbiol 6, 611621. [View Article] [PubMed]
    [Google Scholar]
  3. Connon S. A., Giovannoni S. J. ( 2002 ). High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates. . Appl Environ Microbiol 68, 38783885. [View Article] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  5. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 27, 401410.
    [Google Scholar]
  6. Hedlund B. P., Cho J.-C., Derrien M., Costa K. C. ( 2011 ). Phylum XXII. Lentisphaerae Cho, Vergin, Morris and Giovannoni 2004a, 1005VP (Effective publication: Cho, Vergin, Morris and Giovannoni 2004b, 617.). . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 465467. Edited by Krieg N. R., Staley J. T., Hedlund B. P., Paster B. J., Ward N., Ludwig W., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  7. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 2132. Edited by Munro H. N. . New York:: Academic Press;.
    [Google Scholar]
  8. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.-S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  9. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  11. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  12. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  13. Song J., Oh H.-M., Cho J.-C. ( 2009 ). Improved culturability of SAR11 strains in dilution-to-extinction culturing from the East Sea, West Pacific Ocean. . FEMS Microbiol Lett 295, 141147. [View Article] [PubMed]
    [Google Scholar]
  14. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  15. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  16. Thrash J. C., Cho J.-C., Vergin K. L., Morris R. M., Giovannoni S. J. ( 2010 ). Genome sequence of Lentisphaera araneosa . HTCC2155T , the type species of the order Lentisphaerales in the phylum Lentisphaerae . . J Bacteriol 192, 29382939. [View Article] [PubMed]
    [Google Scholar]
  17. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R. ( 2007 ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  18. Waterbury W. B., Watson S. W., Valois F. W., Franks D. G. ( 1986 ). Biological and ecological characterization of the marine unicellular cyanobacterium Synechococcus . . Can Bull Fish Aquat Sci, 214, 71120. [View Article] [PubMed]
    [Google Scholar]
  19. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  20. Yang S.-J., Cho J.-C. ( 2008 ). Gaetbulibacter marinus sp. nov., isolated from coastal seawater, and emended description of the genus Gaetbulibacter . . Int J Syst Evol Microbiol 58, 315318. [View Article] [PubMed]
    [Google Scholar]
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