1887

Abstract

Two Gram-stain-negative, non-motile, non-pigmented cocci, designated IMCC11369 and IMCC11389, were isolated from surface seawater of the East Sea of Korea by high-throughput cultivation based on dilution to extinction. Strains IMCC11369 and IMCC11389 shared 99.9 % 16S rRNA gene sequence similarity and >86.3 % DNA–DNA relatedness, which suggested that they belong to the same genomic species. The isolates were most closely related to HTCC2155 (99.0 % 16S rRNA gene sequence similarity). Phylogenetic analysis revealed that the isolates formed a robust cluster with HTCC2155. DNA–DNA relatedness values, however, showed that the isolates were distantly related to HTCC2155 (2.0–18.6 %), which suggested that they represent a separate genomic species in the genus . The two isolates were phenotypically differentiated from their closest relative by several characteristics, including degradation of macromolecules and carbon source utilization. The DNA G+C content was 44.5–45.2 mol% and the predominant cellular fatty acids were C, Cω9 and C. Strain IMCC11369 contained MK-7 as the respiratory quinone and phosphatidylethanolamine and an unknown lipid as the major polar lipids. On the basis of data obtained in this study, a novel species is proposed to accommodate the isolates, sp. nov. The type strain is IMCC11369 ( = KCTC 23780 = NBRC 108776).

Funding
This study was supported by the:
  • , National Marine Life Collection Program, Ministry of Land, Transport and Maritime Affairs, Korea , (Award MABIK 2012-001-03)
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2013-04-01
2020-08-08
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