1887

Abstract

During the characterization of the microbiota biodiversity associated with grapes and wineries in different bioclimatic conditions of the Canary Islands (Spain), a novel yeast species was isolated from Lanzarote, the driest wine-producing region of the archipelago. Seven strains isolated from grapes, microvinifications and wineries are described. Sequence analysis of the D1/D2 domain of the LSU rDNA gene and 5.8S-ITS regions revealed that the isolates were phylogenetically a member of the genus and are closely related to NRRL Y-27269 and NRRL Y-48670. On the basis of morphological, biochemical and physiological characterization and phylogenetic analysis, a novel ascosporogenous yeast species, sp. nov., is proposed. The type strain is L2C-15 ( = CBS 12615 = CECT 13066) which was isolated from grape berries of L. cv. Listán Negro red grape variety in Tinajo, Lanzarote. The MycoBank no. is MB 801390.

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2013-01-01
2019-10-23
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References

  1. Altschul S. F. , Madden T. L. , Schäffer A. A. , Zhang J. , Zhang Z. , Miller W. , Lipman D. J. . ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389–3402. [CrossRef] [PubMed]
    [Google Scholar]
  2. Barata A. , González S. S. , Malfeito-Ferreira M. , Querol A. , Loureiro V. . ( 2008; ). Sour rot-damaged grapes are sources of wine spoilage yeasts. . FEMS Yeast Res 8:, 1008–1017. [CrossRef] [PubMed]
    [Google Scholar]
  3. Barata A. , Malfeito-Ferreira M. , Loureiro V. . ( 2012; ). The microbial ecology of wine grape berries. . Int J Food Microbiol 153:, 243–259. [CrossRef] [PubMed]
    [Google Scholar]
  4. del-Arco M. , Acebes J. R. , Pérez-de-Paz P. L. , Marrero M. C. . ( 1999; ). Bioclimatology and climatophilous vegetation of Hierro (part 2) and La Palma (Canary Islands). . Phytocoenologia 29:, 253–290.[CrossRef]
    [Google Scholar]
  5. del-Arco M. , Pérez-de-Paz P. L. , Acebes J. R. , González-Mancebo J. M. , Reyes Betancort J. A. , Bermejo J. A. , de-Armas S. , González González R. . ( 2006; ). Bioclimatology and climatophilous vegetation of Tenerife (Canary Islands). . Ann Bot Fenn 43:, 167–192.
    [Google Scholar]
  6. Esteve-Zarzoso B. , Belloch C. , Uruburu F. , Querol A. . ( 1999; ). Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. . Int J Syst Bacteriol 49:, 329–337. [CrossRef] [PubMed]
    [Google Scholar]
  7. Fell J. W. , Statzell-Tallman A. , Kurtzman C. P. . ( 2004; ). Lachancea meyersii sp. nov., an ascosporogenous yeast from mangrove regions in the Bahama Islands. . Stud Mycol 50:, 359–363.
    [Google Scholar]
  8. Fleet G. , Heard G. M. . ( 1993; ). Yeasts-growth during fermentation. . In Wine Microbiology and Biotechnology, pp. 27–54. Edited by Fleet G. H. . . Philadelphia:: Harwood;.
    [Google Scholar]
  9. Fleet G. , Prakitchaiwattana C. , Beh A. , Heard G. . ( 2002; ). The yeast ecology of wine grapes. . In Biodiversity and Biotechnology of Wine Yeasts, pp. 1–17. Edited by Ciani M. . . India:: Research Signpost;.
    [Google Scholar]
  10. Kurtzman C. P. , Robnett C. J. . ( 1998; ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. . Antonie van Leeuwenhoek 73:, 331–371. [CrossRef] [PubMed]
    [Google Scholar]
  11. Lachance M. A. . ( 2011; ). Lachancea Kurtzman (2003). . In The Yeasts, a Taxonomic Study, , 5th edn., vol. 2, pp. 511–519. Edited by Kurtzman C. P. , Fell J. W. , Boekhout T. . . New York, NY:: Elsevier;. [CrossRef]
    [Google Scholar]
  12. Larkin M. A. , Blackshields G. , Brown N. P. , Chenna R. , McGettigan P. A. , McWilliam H. , Valentin F. , Wallace I. M. , Wilm A. . & other authors ( 2007; ). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lee C. F. , Yao C. H. , Liu Y. R. , Hsieh C. W. , Young S. S. . ( 2009; ). Lachancea dasiensis sp. nov., an ascosporogenous yeast isolated from soil and leaves in Taiwan. . Int J Syst Evol Microbiol 59:, 1818–1822. [CrossRef] [PubMed]
    [Google Scholar]
  14. Mestre M. C. , Ulloa J. R. , Rosa C. A. , Lachance M. A. , Fontenla S. . ( 2010; ). Lachancea nothofagi sp. nov., a yeast associated with Nothofagus species in Patagonia, Argentina. . Int J Syst Evol Microbiol 60:, 2247–2250. [CrossRef] [PubMed]
    [Google Scholar]
  15. Pereira L. F. , Costa C. R. Jr , Brasileiro B. T. , de Morais M. A. Jr . ( 2011; ). Lachancea mirantina sp. nov., an ascomycetous yeast isolated from the cachaca fermentation process. . Int J Syst Evol Microbiol 61:, 989–992. [CrossRef] [PubMed]
    [Google Scholar]
  16. Reyes-Betancort J. A. , Wildpret W. , León Arencibia M. C. . ( 2001; ). The vegetation of Lanzarote (Canary Islands). . Phytocoenologia 31:, 185–247.[CrossRef]
    [Google Scholar]
  17. Samson R. A. , Houbraken J. , Thrane U. , Frisvad J. C. , Andersen B. . ( 2010; ). Food and Indoor Fungi. CBS Laboratory Manual Series no. 2. Utrecht:: CBS-KNAW Fungal Biodiversity Centre;.
    [Google Scholar]
  18. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  19. Yarrow D. . ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. . In The Yeasts, a Taxonomic Study, , 4th edn., pp. 77–100. Edited by Kurtzman C. P. , Fell J. W. . . Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
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