1887

Abstract

Several pectolytic bacterial strains, mainly isolated from monocotyledonous plants and previously identified as , were thought to belong to a novel species after several taxonomic analyses including DNA–DNA hybridization. In 16S rRNA gene sequence analyses, these strains had a similarity of >97.9 % to the 16S rRNA gene sequence of strains representing six other pectobacterial species and subspecies. These strains, represented by strain SCRI 109, also showed some unique chemotaxonomic features and quantitative differences in polar lipids, lipoquinones and fatty acids. A specific feature of strain SCRI 109 was the presence of DMK-8 lipoquinone, while the dominant fatty acids were the summed feature 3 (iso-C 2-OH/Cω7), the unsaturated fatty acid Cω7 and straight chain fatty acids, mainly C. The DNA G+C content of strain SCRI 109 was 50.2 mol%. The taxonomic status of strain SCRI 109 and related strains in 16S rRNA gene sequence, chemotaxonomic, and physiological analyses was corroborated by the distinct clustering of these strains in multi-locus sequence analyses. It is proposed that these strains represent a novel species for which the name sp. nov. is proposed; the type strain is SCRI 109 ( = NCPPB 929 = LMG 2417 = ICMP 1522).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.046011-0
2013-07-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/7/2520.html?itemId=/content/journal/ijsem/10.1099/ijs.0.046011-0&mimeType=html&fmt=ahah

References

  1. Auch A. F., Klenk H.-P., Göker M.. ( 2010;). Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. . Stand Genomic Sci 2:, 142–148. [CrossRef][PubMed]
    [Google Scholar]
  2. Brenner J. D., Steigerwalt G. A., Miklos V. G., Fanning R. G.. ( 1973;). Deoxyribonucleic acid relatedness among Ereiniae and other Enterobacteriaceae: the soft –rot organisms (Genus Pectobacterium Waldee). . Int J Syst Bacteriol 23:, 205–216. [CrossRef]
    [Google Scholar]
  3. Dickey R. S., Kelman A.. ( 1988;). B. Erwinia 2. Carotovora or soft rot group. . In Laboratory Guide for Identification of Plant Pathogenic Bacteria, , 2nd edn., pp. 44–59. Edited by Schaad N. W... St Paul, MN:: The American Phytopatgology Society;.
    [Google Scholar]
  4. Dowson W. J.. ( 1957;). Plant Disease due to Bacteria, , 2nd edn.. Cambridge:: Cambridge University Press;.
    [Google Scholar]
  5. Duarte V., de Boer S. H., Ward L. J., de Oliveira A. M. R.. ( 2004;). Characterization of atypical Erwinia carotovora strains causing blackleg of potato in Brazil. . J Appl Microbiol 96:, 535–545. [CrossRef][PubMed]
    [Google Scholar]
  6. Eldod R. P.. ( 1941;). Serological studies of the Erwinieae, II. Soft rot group; with some biological considerations. . Bot Gaz 103:, 266–279. [CrossRef]
    [Google Scholar]
  7. Gallois A., Samson R., Ageron E., Grimont P. A. D.. ( 1992;). Erwinia carotovora subsp. odorifera subsp. nov., associated with odorous soft rot of chicory (Cichorium intybus L.). . Int J Syst Bacteriol 42:, 582–588. [CrossRef]
    [Google Scholar]
  8. Gardan L., Gouy C., Christen R., Samson R.. ( 2003;). Elevation of three subspecies of Pectobacterium carotovorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov.. Int J Syst Evol Microbiol 53:, 381–391. [CrossRef][PubMed]
    [Google Scholar]
  9. Hasegawa M., Kishino H., Yano T.. ( 1985;). Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. . J Mol Evol 22:, 160–174. [CrossRef][PubMed]
    [Google Scholar]
  10. Hauben L., Moore E. R., Vauterin L., Steenackers M., Mergaert J., Verdonck L., Swings J.. ( 1998;). Phylogenetic position of phytopathogens within the Enterobacteriaceae. . Syst Appl Microbiol 21:, 384–397. [CrossRef][PubMed]
    [Google Scholar]
  11. Holdeman Q. L., Burkholder W. H.. ( 1956;). The identity of barn rots of flue-curried tobacco in South California. . Phytopathology 46:, 69–72.
    [Google Scholar]
  12. Hu X. F., Ying F. X., He Y. B., Gao Y. Y., Chen H. M., Chen J. S.. ( 2008;). Characterization of Pectobacterium carotovorum subsp. carotovoum causing soft-rot disease on Pinellia ternate in China. . Eur J Plant Pathol 120:, 305–310. [CrossRef]
    [Google Scholar]
  13. Ma B., Hibbing M. E., Kim H. S., Reedy R. M., Yedidia I., Breuer J., Breuer J., Glasner J. D., Perna N. T.. & other authors ( 2007;). Host range and molecular phylogenies of the soft rot enterobacterial genera Pectobacterium and Dickeya. . Phytopathology 97:, 1150–1163. [CrossRef][PubMed]
    [Google Scholar]
  14. Nabhan S., De Boer S. H., Maiss E., Wydra K.. ( 2012a;). Taxonomic relatedness between Pectobacterium carotovorum subsp. carotovorum, Pectobacterium carotovorum subsp. odoriferum and Pectobacterium carotovorum subsp. brasiliense subsp. nov.. J Appl Microbiol 113:, 904–913. [CrossRef][PubMed]
    [Google Scholar]
  15. Nabhan A. S., Wydra K., Linde M., Debener T.. ( 2012b;). The use of two complementary DNA assays, AFLP and MLSA, for epidemic and phylogenetic studies of pectolytic enterobacterial strains with focus on the heterogeneous species Pectobacterium carotovorum. . Plant Pathol 61:, 498–508. [CrossRef]
    [Google Scholar]
  16. Perombelon M. C. M.. ( 2002;). Potato diseases caused by soft rot erwinias: an overview of pathogenesis. . Plant Pathol 51:, 1–12. [CrossRef]
    [Google Scholar]
  17. Starr M. P., Mandel M.. ( 1969;). DNA base composition and taxonomy of phyopathogenic and other enterobacteria. . J Gen Microbiol 56:, 113–123. [CrossRef][PubMed]
    [Google Scholar]
  18. Toth I. K., Avrova A. O., Hyman L. J.. ( 2001;). Rapid identification and differentiation of the soft rot erwinias by 16S-23S intergenic transcribed spacer-PCR and restriction fragment length polymorphism analyses. . Appl Environ Microbiol 67:, 4070–4076. [CrossRef][PubMed]
    [Google Scholar]
  19. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  20. Wu J., Diao Y., Gu Y., Hu Z.. ( 2011;). Molecular detection of Pectobacterium species causing soft rot of Amorphophallus konjac. . World J Microbiol Biotechnol 27:, 613–618. [CrossRef]
    [Google Scholar]
  21. Yap M. N., Barak J. D., Charkowski A. O.. ( 2004;). Genomic diversity of Erwinia carotovora subsp. carotovora and its correlation with virulence. . Appl Environ Microbiol 70:, 3013–3023. [CrossRef][PubMed]
    [Google Scholar]
  22. Yedidia I., Ophir R., Yishay M., Ion A., Luzzatto T., Golan A., Burdman S.. ( 2011;). A story of an old battle: Pectobacterium carotovorum and ornamental monocots. . Acta Hort 886:, 417–425.
    [Google Scholar]
  23. Yishay M., Burdman S., Valverde A., Luzzatto T., Ophir R., Yedidia I.. ( 2008;). Differential pathogenicity and genetic diversity among Pectobacterium carotovorum ssp. carotovorum isolates from monocot and dicot hosts support early genomic divergence within this taxon. . Environ Microbiol 10:, 2746–2759. [CrossRef][PubMed]
    [Google Scholar]
  24. Zhu L., Xie H., Chen S., Ma R.. ( 2010;). Rapid isolation, identification and phylogenetic analysis of Pectobacterium carotovorum ssp.. Plant Pathol 92:, 479–483.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.046011-0
Loading
/content/journal/ijsem/10.1099/ijs.0.046011-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error