1887

Abstract

A novel Gram-negative, non-motile, facultative anaerobic and rod-shaped bacterium, designated strain LEN33, was isolated from the intestinal tract of a butterfly (). Strain LEN33 grew optimally at 37 °C in the presence of 1 % (w/v) NaCl and at pH 9. The novel strain was oxidase-negative and catalase-positive. The major cellular fatty acids were C, C and cyclo-C. Strain LEN33 contained two unidentified lipids, three unidentified amino-phospholipids, phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). The major isoprenoid quinone was ubiquinone-8 (Q-8). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LEN33 was most closely related to FRB 97 and NUM 1720, with 98.7 % similarities. DNA–DNA hybridization experiments indicated less than 40.7±2 % relatedness to the closest phylogenetic species, FRB 97. The G+C content of genomic DNA was 58.7 mol%. Phenotypic, phylogenetic and genotypic analysis indicated that strain LEN33 represents a novel species within the genus , for which the name is proposed. The type strain is referred to as LEN33 ( = KACC 16707 = JCM 18389). An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • National Research Foundation of Korea
  • National Institute of Biological Resources (NIBR) (Award 2011-0028854)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.045880-0
2013-07-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/7/2607.html?itemId=/content/journal/ijsem/10.1099/ijs.0.045880-0&mimeType=html&fmt=ahah

References

  1. Bae J. W., Rhee S. K., Park J. R., Chung W. H., Nam Y. D., Lee I., Kim H., Park Y. H. ( 2005 ). Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria. . Appl Environ Microbiol 71, 88258835. [View Article] [PubMed]
    [Google Scholar]
  2. Brady C., Cleenwerck I., Venter S., Vancanneyt M., Swings J., Coutinho T. ( 2008 ). Phylogeny and identification of Pantoea species associated with plants, humans and the natural environment based on multilocus sequence analysis (MLSA). . Syst Appl Microbiol 31, 447460. [View Article] [PubMed]
    [Google Scholar]
  3. Brady C., Denman S., Kirk S., Venter S., Rodríguez-Palenzuela P., Coutinho T. ( 2010 ). Description of Gibbsiella quercinecans gen. nov., sp. nov., associated with Acute Oak Decline. . Syst Appl Microbiol 33, 444450. [View Article] [PubMed]
    [Google Scholar]
  4. Chang H. W., Nam Y. D., Jung M. Y., Kim K. H., Roh S. W., Kim M. S., Jeon C. O., Yoon J. H., Bae J. W. ( 2008 ). Statistical superiority of genome-probing microarrays as genomic DNA–DNA hybridization in revealing the bacterial phylogenetic relationship compared to conventional methods. . J Microbiol Methods 75, 523530. [View Article] [PubMed]
    [Google Scholar]
  5. Collins M. D., Jones D. ( 1981a ). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. . Microbiol Rev 45, 316354. [PubMed]
    [Google Scholar]
  6. Collins M. D., Jones D. ( 1981b ). A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. . J Appl Bacteriol 51, 129134. [View Article] [PubMed]
    [Google Scholar]
  7. Erwin T. L. ( 1982 ). Tropical forests: their richness in Coleoptera and other arthropod species. . Coleopt Bull 36, 7475.
    [Google Scholar]
  8. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  9. Fischbach M. A., Walsh C. T. ( 2009 ). Antibiotics for emerging pathogens. . Science 325, 10891093. [View Article] [PubMed]
    [Google Scholar]
  10. Gonzalez J. M., Saiz-Jimenez C. ( 2002 ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4, 770773. [View Article] [PubMed]
    [Google Scholar]
  11. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  13. Kluge A. G., Farris F. S. ( 1969 ). Quantitative phyletics and the evolution of anurans. . Syst Biol 18, 132. [View Article]
    [Google Scholar]
  14. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . New York:: Wiley;.
    [Google Scholar]
  15. Loy A., Schulz C., Lücker S., Schöpfer-Wendels A., Stoecker K., Baranyi C., Lehner A., Wagner M. ( 2005 ). 16S rRNA gene-based oligonucleotide microarray for environmental monitoring of the betaproteobacterial order “Rhodocyclales” . . Appl Environ Microbiol 71, 13731386. [View Article] [PubMed]
    [Google Scholar]
  16. May R. M., Beverton R. J. H. ( 1990 ). How Many Species?. Philos Trans R Soc Lond B Biol Sci 330, 293304. [View Article]
    [Google Scholar]
  17. MIDI ( 1999 ). Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE:: MIDI, Inc;.
    [Google Scholar]
  18. Rochelle P. A., Fry J. C., Parkes R. J., Weightman A. J. ( 1992 ). DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities. . FEMS Microbiol Lett 79, 5965. [PubMed] [CrossRef]
    [Google Scholar]
  19. Saito M., Shinozaki-Kuwahara N., Takada K. ( 2012 ). Gibbsiella dentisursi sp. nov., isolated from the bear oral cavity. . Microbiol Immunol 56, 506512. [View Article] [PubMed]
    [Google Scholar]
  20. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425. [PubMed]
    [Google Scholar]
  21. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids: MIDI Technical Note 101. Newark, DE:: MIDI;.
    [Google Scholar]
  22. Scully L. R., Bidochka M. J. ( 2006 ). Developing insect models for the study of current and emerging human pathogens. . FEMS Microbiol Lett 263, 19. [View Article] [PubMed]
    [Google Scholar]
  23. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  24. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [View Article] [PubMed]
    [Google Scholar]
  25. Tindall B. J. ( 1990 ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
  26. Tittsler R. P., Sandholzer L. A. ( 1936 ). The use of semi-solid agar for the detection of bacterial motility. . J Bacteriol 31, 575580. [PubMed]
    [Google Scholar]
  27. Vilmos P., Kurucz E. ( 1998 ). Insect immunity: evolutionary roots of the mammalian innate immune system. . Immunol Lett 62, 5966. [View Article] [PubMed]
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  29. Xin H., Itoh T., Zhou P., Suzuki K., Kamekura M., Nakase T. ( 2000 ). Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. . Int J Syst Evol Microbiol 50, 12971303. [View Article] [PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.045880-0
Loading
/content/journal/ijsem/10.1099/ijs.0.045880-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error