1887

Abstract

A novel Gram-negative, non-motile, facultative anaerobic and rod-shaped bacterium, designated strain LEN33, was isolated from the intestinal tract of a butterfly (). Strain LEN33 grew optimally at 37 °C in the presence of 1 % (w/v) NaCl and at pH 9. The novel strain was oxidase-negative and catalase-positive. The major cellular fatty acids were C, C and cyclo-C. Strain LEN33 contained two unidentified lipids, three unidentified amino-phospholipids, phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). The major isoprenoid quinone was ubiquinone-8 (Q-8). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LEN33 was most closely related to FRB 97 and NUM 1720, with 98.7 % similarities. DNA–DNA hybridization experiments indicated less than 40.7±2 % relatedness to the closest phylogenetic species, FRB 97. The G+C content of genomic DNA was 58.7 mol%. Phenotypic, phylogenetic and genotypic analysis indicated that strain LEN33 represents a novel species within the genus , for which the name is proposed. The type strain is referred to as LEN33 ( = KACC 16707 = JCM 18389). An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • , National Research Foundation of Korea
  • , National Institute of Biological Resources (NIBR) , (Award 2011-0028854)
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2013-07-01
2020-08-04
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