1887

Abstract

Three Gram-stain-positive, obligately anaerobic, non-motile, non-spore-forming, spindle-shaped bacterial strains (HT03-11, KO-38 and TT-111), isolated from human faeces were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing showed that the strains were highly related to each other genetically (displaying >99 % sequence similarity) and represented a previously unknown subline within the rRNA group of organisms (cluster XIVa). The closest phylogenetic neighbours of strain HT03-11 were WAL 16351 (93.7 % 16S rRNA gene sequence similarity) and WM1 (93.7 % similarity). All isolates produced lactic acid, formic acid, acetic acid and succinic acid as fermentation end products from glucose. Their chemotaxonomic properties included lysine as the cell wall diamino acid and C, Cω7 DMA and C DMA as the major fatty acids. The G+C contents of the genomic DNA were 46.9–47.2 mol% (HPLC). Several phenotypic and chemotaxonomic characteristics could be readily used to differentiate the isolates from phylogenetically related clostridia. Therefore, strains HT03-11, KO-38 and TT-111 represent a novel species in a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is HT03-11 ( = YIT 12554 = JCM 18507 = DSM 26062).

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2013-10-01
2020-11-24
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