1887

Abstract

A Gram-positive, spore-forming, rod-shaped bacterium, designated J1 was isolated from deep-sea mud collected from the South China Sea and subjected to polyphasic taxonomic investigation. Phylogenetic analysis based on 16S rRNA gene sequences revealed that J1 clustered with the type strains of , and and exhibited a range of similarity of 93.9–97.0 % to members of the genus . The DNA G+C content was 36.7 mol%. Chemotaxonomic analysis showed no quinones, and the cell wall contained -diaminopimelic acid as the diagnostic diamino acid for strain J1. The major cellular fatty acids were iso-C and anteiso-C. Strain J1 was positive for catalase activity and negative for oxidase activity. On the basis of phylogenetic position and phenotypic properties, strain J1 represents a novel species of the genus and the name sp. nov. is proposed. The type strain is J1 ( = CGMCC 1.10434 = JCM 17099).

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2013-04-01
2019-10-13
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References

  1. An S. Y. , Ishikawa S. , Kasai H. , Goto K. , Yokota A. . ( 2007; ). Amphibacillus sediminis sp. nov., an endospore-forming bacterium isolated from lake sediment in Japan. . Int J Syst Evol Microbiol 57:, 2489–2492. [CrossRef] [PubMed]
    [Google Scholar]
  2. Barrow G. I. , Feltham R. K. A. . (editors) ( 1993; ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  3. Bian J. , Song F.-H. , Zhang L.-X. . ( 2008; ). [Strategies on the construction of high-quality microbial natural product library–a review]. . Wei Sheng Wu Xue Bao 48:, 1132–1137 (in Chinese).[PubMed]
    [Google Scholar]
  4. Bian J. , Li Y. , Wang J. , Song F.-H. , Liu M. , Dai H.-Q. , Ren B. , Gao H. , Hu X.-L. . & other authors ( 2009; ). Amycolatopsis marina sp. nov., an actinomycete isolated from an ocean sediment. . Int J Syst Evol Microbiol 59:, 477–481. [CrossRef] [PubMed]
    [Google Scholar]
  5. Buck J. D. . ( 1982; ). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. . Appl Environ Microbiol 44:, 992–993.[PubMed]
    [Google Scholar]
  6. Cao S.-J. , Qu J.-H. , Yang J.-S. , Sun Q. , Yuan H.-L. . ( 2008; ). Halolactibacillus alkaliphilus sp. nov., a moderately alkaliphilic and halophilic bacterium isolated from a soda lake in Inner Mongolia, China, and emended description of the genus Halolactibacillus . . Int J Syst Evol Microbiol 58:, 2169–2173. [CrossRef] [PubMed]
    [Google Scholar]
  7. Chen Y.-G. , Cui X.-L. , Zhang Y.-Q. , Li W.-J. , Wang Y.-X. , Xu L.-H. , Wen M.-L. , Peng Q. , Jiang C.-L. . ( 2009; ). Paraliobacillus quinghaiensis sp. nov., isolated from salt-lake sediment in China. . Int J Syst Evol Microbiol 59:, 28–33. [CrossRef] [PubMed]
    [Google Scholar]
  8. Claus D. , Berkeley R. C. W. . ( 1986; ). Genus Bacillus Cohn 1872. . In Bergey’s Manual of Systematic Bacteriology, vol. 2, pp. 1105–1140. Edited by Sneath P. H. A. , Mair N. S. , Sharpe M. E. , Holt J. G. . . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  9. Collins M. D. , Goodfellow M. , Minnikin D. E. . ( 1980; ). Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curtobacterium and related taxa. . J Gen Microbiol 118:, 29–37.[PubMed]
    [Google Scholar]
  10. Dai H. Q. , Wang J. , Xin Y. H. , Pei G. , Tang S. K. , Ren B. , Ward A. , Ruan J. S. , Li W. J. , Zhang L. X. . ( 2010; ). Verrucosispora sediminis sp. nov., a cyclodipeptide-producing actinomycete from deep-sea sediment. . Int J Syst Evol Microbiol 60:, 1807–1812. [CrossRef] [PubMed]
    [Google Scholar]
  11. Demain A. L. , Zhang L.-X. . ( 2005; ). Natural Products: Drug Discovery and Therapeutics Medicines, pp. 3–56. Edited by Demain A. L. Z. , Zhang L. X. . . Totowa, NJ:: Humana;.
    [Google Scholar]
  12. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: An approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  13. Felsenstein J. . ( 2002; ). phylip (phylogenetic inference package), version 3.6a. Distributed by the author. . Department of Genome Sciences, University of Washington;, Seattle, USA:.
  14. Guindon S. , Dufayard J. F. , Lefort V. , Anisimova M. , Hordijk W. , Gascuel O. . ( 2010; ). New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. . Syst Biol 59:, 307–321. [CrossRef] [PubMed]
    [Google Scholar]
  15. Hasegawa T. , Takizawa M. , Tanida S. . ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29:, 319–322. [CrossRef]
    [Google Scholar]
  16. Hirota K. , Aino K. , Nodasaka Y. , Morita N. , Yumoto I. . ( 2012; ). Amphibacillus indicireducens sp. nov., a facultatively alkaliphile that reduces an indigo dye. . Int J Syst Evol Microbiol 63:, 421–426.[PubMed]
    [Google Scholar]
  17. Horikoshi K. . ( 1999; ). Alkaliphiles: some applications of their products for biotechnology. . Microbiol Mol Biol Rev 63:, 735–750.[PubMed]
    [Google Scholar]
  18. Ishikawa M. , Ishizaki S. , Yamamoto Y. , Yamasato K. . ( 2002; ). Paraliobacillus ryukyuensis gen. nov., sp. nov., a new Gram-positive, slightly halophilic, extremely halotolerant, facultative anaerobe isolated from a decomposing marine alga. . J Gen Appl Microbiol 48:, 269–279. [CrossRef] [PubMed]
    [Google Scholar]
  19. Ishikawa M. , Nakajima K. , Itamiya Y. , Furukawa S. , Yamamoto Y. , Yamasato K. . ( 2005; ). Halolactibacillus halophilus gen. nov., sp. nov. and Halolactibacillus miurensis sp. nov., halophilic and alkaliphilic marine lactic acid bacteria constituting a phylogenetic lineage in Bacillus rRNA group 1. . Int J Syst Evol Microbiol 55:, 2427–2439. [CrossRef] [PubMed]
    [Google Scholar]
  20. Kim S. B. , Falconer C. , Williams E. , Goodfellow M. . ( 1998; ). Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. . Int J Syst Bacteriol 48:, 59–68. [CrossRef] [PubMed]
    [Google Scholar]
  21. Kim O.-S. , Cho Y.-J. , Lee K. , Yoon S.-H. , Kim M. , Na H. , Park S.-C. , Jeon Y. S. , Lee J.-H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  22. Kroppenstedt R. M. . ( 1982; ). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. . J Liquid Chromatogr Relat Technol 5:, 2359–2367. [CrossRef]
    [Google Scholar]
  23. Mandel M. , Marmur J. . ( 1968; ). Use of ultraviolet absorbance temperature profiles for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B:, 195–206. [CrossRef]
    [Google Scholar]
  24. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  25. Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  26. Niimura Y. , Koh E. , Yanagida F. , Suzuki K.-I. , Komagata K. , Kozaki M. . ( 1990; ). Amphibacillus xylanus gen. nov., sp. nov., a facultatively anaerobic spore forming xylan-digesting bacterium which lacks cytochrome, quinone, and catalase. . Int J Syst Bacteriol 40:, 297–301. [CrossRef]
    [Google Scholar]
  27. Pugin B. , Blamey J. M. , Baxter B. K. , Wiegel J. . ( 2012; ). Amphibacillus cookii sp. nov., a facultatively aerobic, spore-forming, moderately halophilic, alkalithermotolerant bacterium. . Int J Syst Evol Microbiol 62:, 2090–2096. [CrossRef] [PubMed]
    [Google Scholar]
  28. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  29. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20:, 16.
    [Google Scholar]
  30. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  31. Simmons J. S. . ( 1926; ). A culture medium for differentiating organisms of typhoid-colon aerogenes groups and for isolation of certain fungi. . J Infect Dis 39:, 209.[CrossRef]
    [Google Scholar]
  32. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  33. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  34. Wang J. , Li Y. , Bian J. , Tang S.-K. , Ren B. , Chen M. , Li W.-J. , Zhang L.-X. . ( 2010; ). Prauserella marina sp. nov., isolated from ocean sediment of the South China Sea. . Int J Syst Evol Microbiol 60:, 985–989. [CrossRef] [PubMed]
    [Google Scholar]
  35. Wu X. Y. , Zheng G. , Zhang W. W. , Xu X. W. , Wu M. , Zhu X. F. . ( 2010; ). Amphibacillus jilinensis sp. nov., a facultatively anaerobic, alkaliphilic bacillus from a soda lake. . Int J Syst Evol Microbiol 60:, 2540–2543. [CrossRef] [PubMed]
    [Google Scholar]
  36. Zhilina T. N. , Garnova E. S. , Turova T. P. , Kostrikina N. A. , Zavarzin G. A. . ( 2001; ). [Amphibacillus fermentum sp. nov., Amphibacillus tropicus sp. nov.–new alkaliphilic, facultatively anaerobic, saccharolytic Bacilli from Lake Magadi]. . Mikrobiologiia 70:, 825–837 (in Russian).[PubMed]
    [Google Scholar]
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