1887

Abstract

A Gram-staining-negative, rod-shaped, non-spore-forming bacterium, designated strain R27, was isolated from the moss , collected from Beijing Songshan National Nature Reserve, China, and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain R27 were Cω7 (33.6 %), C (16.3 %), summed feature 3 (Cω7 and/or Cω6; 15.8 %) and C cyclo (8.7 %) and its major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, three uncharacterized aminolipids and an unknown phospholipid. Strain R27 contained Q-8 as the dominant isoprenoid quinone and the G+C content of its genomic DNA was 64.6 mol%. On the basis of 16S rRNA gene sequence comparison, strain R27 showed 99.1 % similarity to the closest related type strain, OP-1, and 97.6 % similarity to ATCC 29195. However, the DNA–DNA relatedness between strain R27 and CCTCC AB 2010354 and ATCC 29195 was 10.2 and 14.9 %, respectively. Based on 16S rRNA and gene sequence similarities and phenotypic and chemotaxonomic data, strain R27 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R27 ( = CGMCC 1.11013  = DSM 25160).

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2013-06-01
2019-10-19
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References

  1. Aizawa T. , Vijarnsorn P. , Nakajima M. , Sunairi M. . ( 2011; ). Burkholderia bannensis sp. nov., an acid-neutralizing bacterium isolated from torpedo grass (Panicum repens) growing in highly acidic swamps. . Int J Syst Evol Microbiol 61:, 1645–1650. [CrossRef] [PubMed]
    [Google Scholar]
  2. Chen W.-M. , de Faria S. M. , James E. K. , Elliott G. N. , Lin K.-Y. , Chou J.-H. , Sheu S.-Y. , Cnockaert M. , Sprent J. I. , Vandamme P. . ( 2007; ). Burkholderia nodosa sp. nov., isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella . . Int J Syst Evol Microbiol 57:, 1055–1059. [CrossRef] [PubMed]
    [Google Scholar]
  3. Chen W.-M. , de Faria S. M. , Chou J.-H. , James E. K. , Elliott G. N. , Sprent J. I. , Bontemps C. , Young J. P. W. , Vandamme P. . ( 2008; ). Burkholderia sabiae sp. nov., isolated from root nodules of Mimosa caesalpiniifolia . . Int J Syst Evol Microbiol 58:, 2174–2179. [CrossRef] [PubMed]
    [Google Scholar]
  4. Dahllöf I. , Baillie H. , Kjelleberg S. . ( 2000; ). rpoB-based microbial community analysis avoids limitations inherent in 16S rRNA gene intraspecies heterogeneity. . Appl Environ Microbiol 66:, 3376–3380. [CrossRef] [PubMed]
    [Google Scholar]
  5. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  6. Dong X.-Z. , Cai M.-Y. . (editors) ( 2001; ). Determination of biochemical properties. . In Manual for the Systematic Identification of General Bacteria, pp. 370–398. Beijing:: Science Press; (in Chinese).
    [Google Scholar]
  7. Gillis M. , De Ley J. , De Cleene M. . ( 1970; ). The determination of molecular weight of bacterial genome DNA from renaturation rates. . Eur J Biochem 12:, 143–153. [CrossRef] [PubMed]
    [Google Scholar]
  8. Gillis M. , Van Van T. , Bardin R. , Goor M. , Hebbar P. , Willems A. , Segers P. , Kersters K. , Heulin T. , Fernandez M. P. . ( 1995; ). Polyphasic taxonomy in the genus Burkholderia leading to an emended description of the genus and proposition of Burkholderia vietnamiensis sp. nov. for N2-fixing isolates from rice in Vietnam. . Int J Syst Bacteriol 45:, 274–289. [CrossRef]
    [Google Scholar]
  9. Kim O.-S. , Cho Y.-J. , Lee K. , Yoon S.-H. , Kim M. , Na H. , Park S.-C. , Jeon Y. S. , Lee J.-H. et al. ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kroppenstedt R. M. . ( 1982; ). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion-exchanger as stationary phases. . J Liq Chromatogr 5:, 2359–2367. [CrossRef]
    [Google Scholar]
  11. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M. . . Chichester:: Wiley;.
    [Google Scholar]
  12. Li Y. H. , Liu Q. F. , Liu Y. , Zhu J. N. , Zhang Q. . ( 2011; ). Endophytic bacterial diversity in roots of Typha angustifolia L. in the constructed Beijing Cuihu wetland (China). . Res Microbiol 162:, 124–131. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lim Y. W. , Baik K. S. , Han S. K. , Kim S. B. , Bae K. S. . ( 2003; ). Burkholderia sordidicola sp. nov., isolated from the white-rot fungus Phanerochaete sordida . . Int J Syst Evol Microbiol 53:, 1631–1636. [CrossRef] [PubMed]
    [Google Scholar]
  14. Lim J. H. , Baek S.-H. , Lee S.-T. . ( 2008; ). Burkholderia sediminicola sp. nov., isolated from freshwater sediment. . Int J Syst Evol Microbiol 58:, 565–569. [CrossRef] [PubMed]
    [Google Scholar]
  15. Lu P. , Zheng L.-Q. , Sun J.-J. , Liu H.-M. , Li S.-P. , Hong Q. , Li W.-J. . ( 2012; ). Burkholderia zhejiangensis sp. nov., a methyl-parathion-degrading bacterium isolated from a wastewater-treatment system. . Int J Syst Evol Microbiol 62:, 1337–1341. [CrossRef] [PubMed]
    [Google Scholar]
  16. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  17. Marmur J. , Doty P. . ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef] [PubMed]
    [Google Scholar]
  18. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  19. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  20. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  21. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  22. Stackebrandt E. , Frederiksen W. , Garrity G. M. , Grimont P. A. D. , Kämpfer P. , Maiden M. C. J. , Nesme X. , Rosselló-Mora R. , Swings J. et al. ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52:, 1043–1047. [CrossRef] [PubMed]
    [Google Scholar]
  23. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  24. Vanlaere E. , van der Meer J. R. , Falsen E. , Salles J. F. , de Brandt E. , Vandamme P. . ( 2008; ). Burkholderia sartisoli sp. nov., isolated from a polycyclic aromatic hydrocarbon-contaminated soil. . Int J Syst Evol Microbiol 58:, 420–423. [CrossRef] [PubMed]
    [Google Scholar]
  25. Viallard V. , Poirier I. , Cournoyer B. , Haurat J. , Wiebkin S. , Ophel-Keller K. , Balandreau J. . ( 1998; ). Burkholderia graminis sp. nov., a rhizospheric Burkholderia species, and reassessment of [Pseudomonas] phenazinium, [Pseudomonas] pyrrocinia and [Pseudomonas] glathei as Burkholderia . . Int J Syst Bacteriol 48:, 549–563. [CrossRef] [PubMed]
    [Google Scholar]
  26. Yabuuchi E. , Kosako Y. , Oyaizu H. , Yano I. , Hotta H. , Hashimoto Y. , Ezaki T. , Arakawa M. . ( 1992; ). Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov.. Microbiol Immunol 36:, 1251–1275.[PubMed] [CrossRef]
    [Google Scholar]
  27. Yoo S.-H. , Kim B.-Y. , Weon H.-Y. , Kwon S.-W. , Go S.-J. , Stackebrandt E. . ( 2007; ). Burkholderia soli sp. nov., isolated from soil cultivated with Korean ginseng. . Int J Syst Evol Microbiol 57:, 122–125. [CrossRef] [PubMed]
    [Google Scholar]
  28. Zolg W. , Ottow J. C. . ( 1975; ). Pseudomonas glathei sp. nov., a new nitrogen-scavenging rod isolated from acid lateritic relicts in Germany. . Z Allg Mikrobiol 15:, 287–299.[PubMed] [CrossRef]
    [Google Scholar]
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