1887

Abstract

A Gram-stain-positive, coccoid-shaped, halophilic bacterium, strain SR-1, was isolated from a salt crystal obtained from a mine located in Tarija, Bolivia. The strain was investigated using a polyphasic approach. The optimum conditions for growth of strain SR-1 were reached at 5 % (w/v) NaCl, pH 7.6 and 37–40 °C. The peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The isoprenoid quinone was MK-7. The major cellular fatty acids of strain SR-1 were anteiso-C, anteiso-C and iso-C. The DNA G+C content of strain SR-1 was 48.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed a close relationship between strain SR-1 and JCM 2479 (99.7 % 16S rRNA gene sequence similarity), KCTC 19045 (99.4 %) and KCTC 13214 (99.8 %). However, strain SR-1 also showed low levels of DNA–DNA relatedness with these reference strains (47, 61 and 58 %, respectively). On the basis of phenotypic differences and DNA–DNA hybridization results, strain SR-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SR-1 ( = LMG 26930 = CECT 8130).

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2013-09-01
2020-01-22
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References

  1. Bligh E. G., Dyer W. J.. ( 1959;). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol 37:, 911–917. [CrossRef][PubMed]
    [Google Scholar]
  2. Cline S. W., Schalkwyk L. C., Doolittle W. F.. ( 1989;). Transformation of the archaebacterium Halobacterium volcanii with genomic DNA. . J Bacteriol 171:, 4987–4991.[PubMed]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  4. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  5. Hao M. V., Kocur M., Komogata K.. ( 1984;). Marinococcus gen. nov., a new genus for motile cocci with meso-diaminopimelic acid in the cell wall; and Marinococcus albus sp. nov. and Marinococcus halophilus (Novitsky and Kushner) comb. nov.. J Gen Appl Microbiol 30:, 449–459. [CrossRef]
    [Google Scholar]
  6. Hao M. V., Kocur M., Komogata K.. ( 1985;). Marinococcus gen. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 19. . Int J Syst Bacteriol 35:, 535. [CrossRef]
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  8. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A.. & other authors ( 2007;). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef][PubMed]
    [Google Scholar]
  9. Li W.-J., Schumann P., Zhang Y.-Q., Chen G.-Z., Tian X.-P., Xu L.-H., Stackebrandt E., Jiang C.-L.. ( 2005;). Marinococcus halotolerans sp. nov., isolated from Qinghai, north-west China. . Int J Syst Evol Microbiol 55:, 1801–1804. [CrossRef][PubMed]
    [Google Scholar]
  10. Marquez M. C., Ventosa A., Ruiz-Berraquero F.. ( 1990;). Marinococcus hispanicus, a new species of moderately halophilic gram-positive cocci. . Int J Syst Bacteriol 40:, 165–169. [CrossRef]
    [Google Scholar]
  11. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  12. Novitsky T. J., Kushner D. J.. ( 1976;). Planococcus halophilus sp. nov., a facultatively halophilic coccus. . Int J Syst Bacteriol 26:, 53–57. [CrossRef]
    [Google Scholar]
  13. Quillaguamán J., Hatti-Kaul R., Mattiasson B., Alvarez M. T., Delgado O.. ( 2004;). Halomonas boliviensis sp. nov., an alkalitolerant, moderate halophile isolated from soil around a Bolivian hypersaline lake. . Int J Syst Evol Microbiol 54:, 721–725. [CrossRef][PubMed]
    [Google Scholar]
  14. Quillaguamán J., Delgado O., Mattiasson B., Hatti-Kaul R.. ( 2006;). Poly(β-hydroxybutyrate) production by a moderate halophile, Halomonas boliviensis LC1. . Enzyme Microb Technol 38:, 148–154. [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  16. Schaeffer A. B., Fulton M. D.. ( 1933;). A simplified method of staining endospores. . Science 77:, 194. [CrossRef][PubMed]
    [Google Scholar]
  17. Schleifer K. H.. ( 1985;). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18:, 123–156. [CrossRef]
    [Google Scholar]
  18. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  19. Silvestro D., Michalak I.. ( 2012;). raxmlGUI: a graphical front-end for RAxML. . Org Divers Evol 12:, 335–337. [CrossRef]
    [Google Scholar]
  20. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 611–651. Edited by Gerhart P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Stamatakis A., Ludwig T., Meier H.. ( 2005;). RAxML-III: a fast program for maximum likelihood-based inference of large phylogenetic trees. . Bioinformatics 21:, 456–463. [CrossRef][PubMed]
    [Google Scholar]
  22. Tamaoka J.. ( 1986;). Analysis of bacterial menaquinone mixtures by reverse-phase high-performance liquid chromatography. . Methods Enzymol 123:, 251–256. [CrossRef][PubMed]
    [Google Scholar]
  23. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  24. Ventosa A., Quesada E., Rodríguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A.. ( 1982;). Numerical taxonomy of moderately halophilic Gram-negative rods. . J Gen Microbiol 128:, 1959–1968.
    [Google Scholar]
  25. Ventosa A., Marquez M. C., Weiss N., Tindall B. J.. ( 1992;). Transfer of Marinococcus hispanicus to the genus Salinicoccus as Salinicoccus hispanicus comb. nov.. Syst Appl Microbiol 15:, 530–534. [CrossRef]
    [Google Scholar]
  26. Ventosa A., Nieto J. J., Oren A.. ( 1998;). Biology of moderately halophilic aerobic bacteria. . Microbiol Mol Biol Rev 62:, 504–544.[PubMed]
    [Google Scholar]
  27. Wang Y., Cao L.-L., Tang S.-K., Lou K., Mao P.-H., Jin X., Jiang C.-L., Xu L.-H., Li W.-J.. ( 2009;). Marinococcus luteus sp. nov., a halotolerant bacterium isolated from a salt lake, and emended description of the genus Marinococcus. . Int J Syst Evol Microbiol 59:, 2875–2879. [CrossRef][PubMed]
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  29. Yoon J. H., Kang S. J., Oh T. K.. ( 2007;). Reclassification of Marinococcus albus Hao et al. 1985 as Salimicrobium album gen. nov., comb. nov. and Bacillus halophilus Ventosa et al. 1990 as Salimicrobium halophilum comb. nov., and description of Salimicrobium luteum sp. nov.. Int J Syst Evol Microbiol 57:, 2406–2411. [CrossRef][PubMed]
    [Google Scholar]
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