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Abstract

A Gram-stain-positive, coccoid-shaped, halophilic bacterium, strain SR-1, was isolated from a salt crystal obtained from a mine located in Tarija, Bolivia. The strain was investigated using a polyphasic approach. The optimum conditions for growth of strain SR-1 were reached at 5 % (w/v) NaCl, pH 7.6 and 37–40 °C. The peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The isoprenoid quinone was MK-7. The major cellular fatty acids of strain SR-1 were anteiso-C, anteiso-C and iso-C. The DNA G+C content of strain SR-1 was 48.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed a close relationship between strain SR-1 and JCM 2479 (99.7 % 16S rRNA gene sequence similarity), KCTC 19045 (99.4 %) and KCTC 13214 (99.8 %). However, strain SR-1 also showed low levels of DNA–DNA relatedness with these reference strains (47, 61 and 58 %, respectively). On the basis of phenotypic differences and DNA–DNA hybridization results, strain SR-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SR-1 ( = LMG 26930 = CECT 8130).

Funding
This study was supported by the:
  • Swedish International Development Cooperation Agency (Sida)
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2013-09-01
2024-12-08
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