1887

Abstract

A micro-organism resembling members of the genus , strain W254, which was isolated from the submandibular lymph node of a pig, and an additional 16 strains isolated from swine tonsils, were studied to establish their taxonomic status. Although all 17 strains were isolated anaerobically under an atmosphere of 100 % CO, all of them were aerotolerant anaerobes. The micro-organisms showed at least five cellular morphologies: (i) a radially protrusive thallus, which proliferated into tuber-like cells; (ii) segmentation in both tubers and thallus followed by multilocule formation, (iii) development of coccoid forms in the locules; (iv) a change from the coccoid forms to zoospores; (v) resting cells, which were able to develop into protrusive thalli again. The micro-organisms were positive for nitrate reduction, but negative for catalase, indole production, hydrolysis of urea and gelatin liquefaction. Milk was not decomposed and none of the strains was haemolytic. A total of 16 compounds, including glucose, were utilized as sole carbon sources and seven compounds, including -arabinose, were not utilized. Three out of the 17 strains were subjected to further studies. The micro-organisms had -diaminopimelic acid in their peptidoglycan and galactose, glucose, madurose and a trace of mannose in their whole-cell sugar patterns. The major phospholipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol.Cellular fatty acids were C (35.7–23.1 %), C (5.9–2.4 %) C (62.9–39.5 %), C (24.4–0 %) and C (3–1.6 %). The predominant menaquinone was MK-8 (H). The G+C content of the DNA was 69.6–71.8 mol%. Analysis of the 16S rRNA gene sequences showed that the strains clustered with the type strains of members of the family . Based on the polyphasic taxonomic characterization carried out, all 17 strains are considered to belong to a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is W254 ( = ATCC 35846 = CCM 3774 = DSM 21880 = JCM 15727).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.045237-0
2013-07-01
2019-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/7/2545.html?itemId=/content/journal/ijsem/10.1099/ijs.0.045237-0&mimeType=html&fmt=ahah

References

  1. Abu-Samra M. T. , Imbabi S. E. , Mahgoub E. S. . ( 1976; ). Dermatophilus congolensis. A bacteriological, in vitro antibiotic sensitivity and histopathological study of natural infection in Sudanese cattle. . Br Vet J 132:, 627–631.[PubMed]
    [Google Scholar]
  2. Anonymous ( 1989; ). Lactophenol Method. . In Veterinary Dictionary, p. 1545. Tokyo:: Chikusan Publishing;.
    [Google Scholar]
  3. Azuma R. , Bak U. B. . ( 1980; ). Isolation of Dermatophilus-like microorganisms from swine tonsils. . Proc Int Pig Vet Sci 6th Congress, Cophenhagen. 348.
    [Google Scholar]
  4. Azuma R. , Ito T. . ( 1987; ). Botulism in waterfowl and distribution of C. botulism Type C in Japan. . In Avian botulism: an International Perspective, pp. 167–187. Edited by Ekuland M. L. , Dowell V. R. . . Springfield, IL:: Charles C Thomas;.
    [Google Scholar]
  5. Azuma R. , Suto T. . ( 1970; ). Validity of transfer of the taxonomical position of Corynebacterium pseudopyogenes from Genus Corynebacterium to Genus Actinomyces . . In Culture Collection of Microorganism. 493–505 Edited by Iizuka H. , Hasegawa T. . : Proc 1st Int Conf Cult Collect Univ Tokyo Press, Tokyo:.
    [Google Scholar]
  6. Azuma R. , Takahashi Y. , Miyagawa E. , Taniguchi T. . ( 1989; ). Additional findings on Dermatophilus like organism, ‘Tonsillophilus suis’ from swine tonsils. . In Trends in Actinomycetology in Japan, pp. 21–24. Edited by Koyama Y . . Japan:: Society for Actinomycetes;.
    [Google Scholar]
  7. Azuma R. , Koeda T. , Murakami S. , Shiozawa M. , Fujiwara H. . ( 1990; ). Tonsillophilus suis ‘infection among Japanese pigs. . Proc Int Pig Vet Sci 11th Congress, Lausanne:. 192.
    [Google Scholar]
  8. Azuma R. , Ishiwata H. , Yoshikawa F. , Watanabe T. , Oomi T. , Yamamoto T. , Kudo T. , Kusunoki H. , Makino T. . ( 1992; ). Selective medium and some chemotaxonomical findings on ‘Tonsillophilus suis’. . Proc Int Pig Vet Sci 12th Congress 346.
    [Google Scholar]
  9. Azuma R. , Murakami S. , Ogawa A. , Okada Y. , Miyazaki S. , Makino T. . ( 2009; ). Arcanobacterium abortisuis sp. nov., isolated from a placenta of a sow following an abortion. . Int J Syst Evol Microbiol 59:, 1469–1473. [CrossRef] [PubMed]
    [Google Scholar]
  10. Bak U. B. , Azuma R. . ( 1980; ). Dermatophilus infection of porcine tonsils. . Proc Int Pig Vet Sci 6th Congress, Copenhagen, 349.
  11. Bak U. B. , Azuma R. . ( 1991; ). Pathology of infection caused by Dermatophilus-like organisms in porcine tonsils. . J Comp Pathol 105:, 255–262. [CrossRef] [PubMed]
    [Google Scholar]
  12. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef] [PubMed]
    [Google Scholar]
  13. Cote R. . ( 1992; ). Media formulation 1402, MVL. . In American Type Culture Collection., Catalogue of Bacteria and Fungi, , 472nd edn., p. 472. Edited by Ghena R. , Pienta P. , Cote R. . . Rockville, MD:: The American Type Culture Collection;.
    [Google Scholar]
  14. Cowan S. T. . ( 1974; ). Cowan & Steel’s Manual for the Identification of Medical Bacteria. Cambridge:: Cambridge University Press;.
    [Google Scholar]
  15. Davis D. J. . ( 1923; ). A comparative study of the crypt content of tonsils of man, pig and cow. . JAMA 81:, 1172–1174. [CrossRef]
    [Google Scholar]
  16. Ezaki T. , Saidi S. M. , Liu S. L. , Hashimoto Y. , Yamamoto H. , Yabuuchi E. . ( 1990; ). Rapid procedure to determine the DNA base composition from small amounts of Gram-positive bacteria. . FEMS Microbiol Lett 67:, 127–130. [CrossRef] [PubMed]
    [Google Scholar]
  17. Folch J. , Lees M. , Sloane Stanley G. H. . ( 1957; ). A simple method for the isolation and purification of total lipids from animal tissues. . J Biol Chem 226:, 497–509.[PubMed]
    [Google Scholar]
  18. Gilka F. . ( 1983; ). Electron microscopy of radiating clubs in tonsillar crypts of swine. . Vet Pathol 20:, 57–64.[PubMed]
    [Google Scholar]
  19. Gordon M. A. . ( 1964; ). The genus Dermatophilus . . J Bacteriol 88:, 509–522.[PubMed]
    [Google Scholar]
  20. Gordon M. A. . ( 1989; ). Genus Dermatophilus . . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2409–2410. Edited by Williams S. T. , Sharpe M. E. , Holt J. G. . . Baltimore, MD:: Williams & Wilkins;.
    [Google Scholar]
  21. Hamada M. , Iino T. , Iwami T. , Harayama S. , Tamura T. , Suzuki K. . ( 2010; ). Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov.. J Gen Appl Microbiol 56:, 427–436. [CrossRef] [PubMed]
    [Google Scholar]
  22. Hyslop N. S. . ( 1979; ). Dermatophilosis (streptothricosis) in animals and man. . Comp Immunol Microbiol Infect Dis 2:, 389–404. [CrossRef] [PubMed]
    [Google Scholar]
  23. Johne F. . ( 1881; ). Weitereszur Kenntisniss des Stralenpilzes (Actinomyces bovis). . Centralbl F Med Wissensch 15:, 273–274.
    [Google Scholar]
  24. Johne F. . ( 1882; ). Die Actinomykose oder Strahlenpilzerkrankung, eine neue Infectioneskrankheit. . Deutsche Zeitschlift f Thiermed u vergl Pathologie VII, 141–192.
    [Google Scholar]
  25. Kinjo E. , Motonaga H. , Matayoshi E. , Kudo S. , Watanabe K. , Hara M. , Tabuchi K. , Momotani E. , Azuma R. . ( 1981; ). Occurrence of bovine dermatophilosis in the sourthernmost islands of Japan. . Natl Inst Anim Health Q (Tokyo) 21:, 163–174.
    [Google Scholar]
  26. Kohn J. . ( 1953; ). A preliminary report of a new gelatin liquefaction method. . J Clin Pathol 6:, 249. [CrossRef] [PubMed]
    [Google Scholar]
  27. Momotani E. , Yoshino T. , Ishikawa Y. , Azuma R. . ( 1983; ). Morphology of experimental actinomycotic abscess in mice with Dermatophilus-like microorganisms from porcine tonsil. . Mycopathologia 81:, 99–105. [CrossRef] [PubMed]
    [Google Scholar]
  28. Momotani E. , Inui S. , Ishikawa Y. , Azuma R. . ( 1985; ). Experimental subcutaneous granulomas in sheep with Dermatophilus-like microorganisms from porcine tonsil. . Mycopathologia 91:, 143–149. [CrossRef] [PubMed]
    [Google Scholar]
  29. Murakami S. , Okazaki Y. , Shiozawa M. , Oishi H. , Koeda T. , Gotou K. , Azuma R. , Fujiwara H. . ( 1991; ). Tonsillar abscess caused by “Tonsillophilus suis” infection in fattening pigs. . J Vet Med Sci 53:, 755–757. [CrossRef] [PubMed]
    [Google Scholar]
  30. Murakami S. , Koeda T. , Azuma R. , Fujiwara H. . ( 1992; ). Experimental Tonsillophilus suis granuloma in rats and guinea pigs. . J Vet Med Sci 54:, 891–895. [CrossRef] [PubMed]
    [Google Scholar]
  31. Murakami S. , Azuma R. , Oomi H. , Koeda T. , Fujiwara H. . ( 1997; ). Incidence of tonsillar lesions caused by Tonsillophilus suis and Actinomyces sp infection in swine. . Zentralbl Veterinarmed A 44:, 611–618.[PubMed] [CrossRef]
    [Google Scholar]
  32. Murakami S. , Azuma R. , Koeda T. , Oomi H. , Watanabe T. , Fujiwara H. . ( 1998; ). Immunohistochemical detection for Actinomyces sp. in swine tonsillar abscess and granulomatous mastitis. . Mycopathologia 141:, 15–19. [CrossRef] [PubMed]
    [Google Scholar]
  33. Ogura K. . ( 1925; ). On pathogenic Actinomycetes (the 1st report). . Jap J. Bacteriol 359:, 1–19.
    [Google Scholar]
  34. Oomi, H., Azuma, R., Ishiwata, H., Asahara, T., Watanabe, T. & Murakami, S. (1994). Bacteriological study on another pathogenic agent ‘Actinomyces suis’ Grasser 1957 in swine tonsils. Proc Int Pig Vet Sci 13th Congress, Bangkok, 323.
  35. Research Group ( 1951; ). Flagella staining. Toda’s method. . Practical Bacteriological Text. Edited by Researchers of the National Institute of Infectious Disease . Tokyo:: Maruzen Publishing;.
    [Google Scholar]
  36. Rohde P. A. . ( 1973; ). Trypticase Soy Broth. In: BBL Manual of Products and Laboratory Procedures. p.151. Cockeysville, MD:: BBL;
    [Google Scholar]
  37. Saiki R. K. , Gelfand D. H. , Stoffel S. , Scharf S. J. , Higuchi R. , Horn G. T. , Mullis K. B. , Erlich H. A. . ( 1988; ). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. . Science 239:, 487–491. [CrossRef] [PubMed]
    [Google Scholar]
  38. Schumann P. , Kämpfer P. , Busse H.-J. , Evtushenko L. I. . Subcommittee on the Taxonomy of the Suborder Micrococcineae of the International Committee on Systematics of Prokaryotes ( 2009; ). Proposed minimal standards for describing new genera and species of the suborder Micrococcineae . . Int J Syst Evol Microbiol 59:, 1823–1849. [CrossRef] [PubMed]
    [Google Scholar]
  39. Shiozawa M. , Koizumi H. , Ito S. , Harada Y. , Imagawa T. , Murakami S. , Koeda T. , Azuma R. , Fujiwara H. . ( 1991; ). Incidence of tonsillitis by Tonsillophilus suis infection of fattening and breeding pigs. . Nippon Juishikai Zasshi 44:, 1093–1097. (in Japanese with English summary).
    [Google Scholar]
  40. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  41. Tamura, T., Nakagawa, Y. & Kawasaki, H. (2000). DNA–DNA hybridization. In: Identification Manual of Actinomycetes. pp. 134–137. Edited by the Society for Actinomycetes. Tokyo: Business Center for Academic Societies. (in Japanese).
  42. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  43. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.045237-0
Loading
/content/journal/ijsem/10.1099/ijs.0.045237-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error