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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 63, Issue Pt_7

gen. nov., sp. nov., causing tonsil infections in pigs Open Access

Abstract

A micro-organism resembling members of the genus , strain W254, which was isolated from the submandibular lymph node of a pig, and an additional 16 strains isolated from swine tonsils, were studied to establish their taxonomic status. Although all 17 strains were isolated anaerobically under an atmosphere of 100 % CO, all of them were aerotolerant anaerobes. The micro-organisms showed at least five cellular morphologies: (i) a radially protrusive thallus, which proliferated into tuber-like cells; (ii) segmentation in both tubers and thallus followed by multilocule formation, (iii) development of coccoid forms in the locules; (iv) a change from the coccoid forms to zoospores; (v) resting cells, which were able to develop into protrusive thalli again. The micro-organisms were positive for nitrate reduction, but negative for catalase, indole production, hydrolysis of urea and gelatin liquefaction. Milk was not decomposed and none of the strains was haemolytic. A total of 16 compounds, including glucose, were utilized as sole carbon sources and seven compounds, including -arabinose, were not utilized. Three out of the 17 strains were subjected to further studies. The micro-organisms had -diaminopimelic acid in their peptidoglycan and galactose, glucose, madurose and a trace of mannose in their whole-cell sugar patterns. The major phospholipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol.Cellular fatty acids were C (35.7–23.1 %), C (5.9–2.4 %) C (62.9–39.5 %), C (24.4–0 %) and C (3–1.6 %). The predominant menaquinone was MK-8 (H). The G+C content of the DNA was 69.6–71.8 mol%. Analysis of the 16S rRNA gene sequences showed that the strains clustered with the type strains of members of the family . Based on the polyphasic taxonomic characterization carried out, all 17 strains are considered to belong to a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is W254 ( = ATCC 35846 = CCM 3774 = DSM 21880 = JCM 15727).

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2013-07-01
2025-04-20
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Tonsilliphilus suis gen. nov., sp. nov., causing tonsil infections in pigs
Int J Syst Evol Microbiol 63, 2545 (2013); https://doi.org/10.1099/ijs.0.045237-0
/content/journal/ijsem/10.1099/ijs.0.045237-0
/content/journal/ijsem/10.1099/ijs.0.045237-0
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