1887

Abstract

A species diversity study of lactic acid bacteria occurring in traditional Vietnamese nem chua yielded an isolate, LMG 26767, that could not be assigned to a species with a validly published name. The isolate was initially investigated by 16S rRNA gene sequence analysis, which revealed that it belonged to the genus , with and as the closest relatives (98.9 % and 96.9 % gene sequence similarity to the type strains, respectively). Comparative (GTG)-PCR genomic fingerprinting confirmed the unique taxonomic status of the novel strain. DNA–DNA hybridization experiments, DNA G+C content determination, sequence analysis of the phenylalanyl-tRNA synthase () gene, and physiological and biochemical characterization demonstrated that strain LMG 26767 represents a novel species, for which the name sp. nov. is proposed; the type strain is LMG 26767 ( = CCUG 62266). Biochemically, can be distinguished from and by its carbohydrate fermentation profile, absence of growth at 45 °C, and production of - and -lactate as end products of glucose metabolism.

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2013-05-01
2020-01-18
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