1887

Abstract

The bacterial strain C3 was isolated from permafrost soil in Beijicun, Mohe County, Heilongjiang province, China. Cells of strain C3 were Gram-stain-negative rods, 0.3–0.4 µm in diameter and 1.0–2.6 µm in length. Strain C3 was strictly aerobic. Growth occurred at 15–37 °C but not at 4 or 42 °C, at pH 5.0–9.0 (optimum pH 6.0–7.0) and in the presence of 0–8 g NaCl l (optimum 0–1 g l). The analysis of 16S rRNA gene sequences indicated that strain C3 was phylogenetically related to members of the genus , with similarities ranging from 94.7 to 96.5 %. Strain C3 contained ubiquinone 8 as the major respiratory quinone. The major cellular fatty acids were summed feature 3 (Cω7/Cω6), C cyclo, straight-chain C, C and C, unsaturated Cω7 and hydroxylated fatty acids C 3-OH and C 2-OH. The polar lipids were mainly phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The polyamines were putrescine and 2-hydroxyputrescine. The DNA G+C content was 57.4 mol% (determined from ). Based on these results, it is concluded that strain C3 represents a novel species of the genus , for which the name sp. nov. is proposed, with C3 ( = CGMCC 1.10998 = NBRC 108789) representing the type strain.

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2013-06-01
2019-10-22
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