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Abstract

A bacterial strain designated Ruye-90 was isolated from a freshwater tilapiine cichlid fish culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Strain Ruye-90 was Gram-negative, aerobic, yellow-coloured, rod-shaped, and motile by means of a single polar flagellum. Growth occurred at 4–30 °C (optimum, 20–30 °C), at pH 7.0–9.0 (optimum, pH 8.0–9.0) and with 0–2 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Ruye-90 belonged to the genus and its most closely related neighbour was JA3-B52 with sequence similarity of 97.5 %. The major fatty acids were summed feature 3 (comprising Cω7 and/or Cω6, 41.3 %), C (19.3 %), Cω7 (8.4 %) and C 3-OH (7.0 %). The major respiratory quinone was Q-8. The DNA G+C content of the genomic DNA was 49.0 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, aminolipid and two uncharacterized phospholipids. The DNA–DNA relatedness of strain Ruye-90 with respect to recognized members of the genus was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Ruye-90 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is Ruye-90 ( = LMG 26339 = BCRC 80263 = KCTC 23315).

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2013-04-01
2020-10-20
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