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Abstract

A Gram-type-negative, obligately anaerobic, selenate-respiring bacterium, strain S4, was isolated from activated sludge of a wastewater treatment plant in New Jersey after enrichment with 10 mM selenate as the sole electron acceptor. In addition to its selenate-respiring capability, strain S4 also respired arsenate with acetate as carbon source and electron donor. Fermentative growth was not observed. The optimum growth temperature was 37 °C and optimum pH was pH 7. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain S4 is a novel member of the family , with the type strain of as its closest cultivated relative, with 91.5 % sequence similarity. The cellular fatty acid profile was composed predominantly of straight-chain fatty acids C, C, C, C and C, which distinguishes this organism from its closest relatives. The DNA G+C content was 47.7 mol%. Together, these findings support the conclusion that strain S4 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is S4 ( = DSM 24984 = ATCC BAA-2290).

Funding
This study was supported by the:
  • , National Science Foundation , (Award EAR 084329530)
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2013-10-01
2020-10-27
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