1887

Abstract

A Gram-type-negative, obligately anaerobic, selenate-respiring bacterium, strain S4, was isolated from activated sludge of a wastewater treatment plant in New Jersey after enrichment with 10 mM selenate as the sole electron acceptor. In addition to its selenate-respiring capability, strain S4 also respired arsenate with acetate as carbon source and electron donor. Fermentative growth was not observed. The optimum growth temperature was 37 °C and optimum pH was pH 7. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain S4 is a novel member of the family , with the type strain of as its closest cultivated relative, with 91.5 % sequence similarity. The cellular fatty acid profile was composed predominantly of straight-chain fatty acids C, C, C, C and C, which distinguishes this organism from its closest relatives. The DNA G+C content was 47.7 mol%. Together, these findings support the conclusion that strain S4 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is S4 ( = DSM 24984 = ATCC BAA-2290).

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2013-10-01
2019-10-17
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. ( 1997;). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389–3402. [CrossRef][PubMed]
    [Google Scholar]
  2. Caccavo F. Jr, Coates J. D., Rossello-Mora R. A., Ludwig W., Schleifer K. H., Lovley D. R., McInerney M. J.. ( 1996;). Geovibrio ferrireducens, a phylogenetically distinct dissimilatory Fe(III)-reducing bacterium. . Arch Microbiol 165:, 370–376. [CrossRef][PubMed]
    [Google Scholar]
  3. Chenna R., Sugawara H., Koike T., Lopez R., Gibson T. J., Higgins D. G., Thompson J. D.. ( 2003;). Multiple sequence alignment with the clustal series of programs. . Nucleic Acids Res 31:, 3497–3500. [CrossRef][PubMed]
    [Google Scholar]
  4. Fennell D. E., Rhee S. K., Ahn Y. B., Häggblom M. M., Kerkhof L. J.. ( 2004;). Detection and characterization of a dehalogenating microorganism by terminal restriction fragment length polymorphism fingerprinting of 16S rRNA in a sulfidogenic, 2-bromophenol-utilizing enrichment. . Appl Environ Microbiol 70:, 1169–1175. [CrossRef][PubMed]
    [Google Scholar]
  5. Fiala G., Woese C. R., Langworthy T. A., Stetter K. O.. ( 1990;). Flexistipes sinusarabici, a novel genus and species of eubacteria occurring in the Atlantic II Deep brines of the Red Sea. . Arch Microbiol 154:, 120–126. [CrossRef]
    [Google Scholar]
  6. Garrity G. M., Holt J. M.. ( 2001;). Phylum BIX. Deferribacteres phy. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 1, pp. 465–471. Edited by Boone D. R., R. W., Castenholz G. M., Garrity.. New York:: Springer;. [CrossRef]
    [Google Scholar]
  7. Greene A. C., Patel B. K. C., Sheehy A. J.. ( 1997;). Deferribacter thermophilus gen. nov., sp. nov., a novel thermophilic manganese- and iron-reducing bacterium isolated from a petroleum reservoir. . Int J Syst Bacteriol 47:, 505–509. [CrossRef][PubMed]
    [Google Scholar]
  8. Iino T., Nakagawa T., Mori K., Harayama S., Suzuki K.. ( 2008;). Calditerrivibrio nitroreducens gen. nov., sp. nov., a thermophilic, nitrate-reducing bacterium isolated from a terrestrial hot spring in Japan. . Int J Syst Evol Microbiol 58:, 1675–1679. [CrossRef][PubMed]
    [Google Scholar]
  9. Janssen P. H., Liesack W., Schink B.. ( 2002;). Geovibrio thiophilus sp. nov., a novel sulfur-reducing bacterium belonging to the phylum Deferribacteres. . Int J Syst Evol Microbiol 52:, 1341–1347. [CrossRef][PubMed]
    [Google Scholar]
  10. Kerkhof L., Ward B. B.. ( 1993;). Comparison of nucleic acid hybridization and fluorometry for measurement of the relationship between RNA/DNA ratio and growth rate in a marine bacterium. . Appl Environ Microbiol 59:, 1303–1309.[PubMed]
    [Google Scholar]
  11. Männistö M. K., Tiirola M., McConnell J., Häggblom M. M.. ( 2010;). Mucilaginibacter frigoritolerans sp. nov., Mucilaginibacter lappiensis sp. nov. and Mucilaginibacter mallensis sp. nov., isolated from soil and lichen samples. . Int J Syst Evol Microbiol 60:, 2849–2856. [CrossRef][PubMed]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  13. Miroshnichenko M. L., Slobodkin A. I., Kostrikina N. A., L’Haridon S. L., Nercessian O., Spring S., Stackebrandt E., Bonch-Osmolovskaya E. A., Jeanthon C.. ( 2003;). Deferribacter abyssi sp. nov., an anaerobic thermophile from deep-sea hydrothermal vents of the Mid-Atlantic Ridge. . Int J Syst Evol Microbiol 53:, 1637–1641. [CrossRef][PubMed]
    [Google Scholar]
  14. Muyzer G., de Waal E. C., Uitterlinden A. G.. ( 1993;). Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. . Appl Environ Microbiol 59:, 695–700.[PubMed]
    [Google Scholar]
  15. Myhr S., Torsvik T.. ( 2000;). Denitrovibrio acetiphilus, a novel genus and species of dissimilatory nitrate-reducing bacterium isolated from an oil reservoir model column. . Int J Syst Evol Microbiol 50:, 1611–1619. [CrossRef][PubMed]
    [Google Scholar]
  16. Narasingarao P., Häggblom M. M.. ( 2006;). Sedimenticola selenatireducens, gen. nov., sp. nov., an anaerobic selenate-respiring bacterium isolated from estuarine sediment. . Syst Appl Microbiol 29:, 382–388. [CrossRef][PubMed]
    [Google Scholar]
  17. Narasingarao P., Häggblom M. M.. ( 2007;). Identification of anaerobic selenate-respiring bacteria from aquatic sediments. . Appl Environ Microbiol 73:, 3519–3527. [CrossRef][PubMed]
    [Google Scholar]
  18. Rauschenbach I., Narasingarao P., Häggblom M. M.. ( 2011;). Desulfurispirillum indicum sp. nov., a selenate- and selenite-respiring bacterium isolated from an estuarine canal. . Int J Syst Evol Microbiol 61:, 654–658. [CrossRef][PubMed]
    [Google Scholar]
  19. Robertson B. R., O’Rourke J. L., Neilan B. A., Vandamme P., On S. L. W., Fox J. G., Lee A.. ( 2005;). Mucispirillum schaedleri gen. nov., sp. nov., a spiral-shaped bacterium colonizing the mucus layer of the gastrointestinal tract of laboratory rodents. . Int J Syst Evol Microbiol 55:, 1199–1204. [CrossRef][PubMed]
    [Google Scholar]
  20. Slobodkina G. B., Kolganova T. V., Chernyh N. A., Querellou J., Bonch-Osmolovskaya E. A., Slobodkin A. I.. ( 2009;). Deferribacter autotrophicus sp. nov., an iron(III)-reducing bacterium from a deep-sea hydrothermal vent. . Int J Syst Evol Microbiol 59:, 1508–1512. [CrossRef][PubMed]
    [Google Scholar]
  21. Stolz J. F., Oremland R. S.. ( 1999;). Bacterial respiration of arsenic and selenium. . FEMS Microbiol Rev 23:, 615–627. [CrossRef][PubMed]
    [Google Scholar]
  22. Stolz J. F., Basu P., Santini J. M., Oremland R. S.. ( 2006;). Arsenic and selenium in microbial metabolism. . Annu Rev Microbiol 60:, 107–130. [CrossRef][PubMed]
    [Google Scholar]
  23. Takai K., Kobayashi H., Nealson K. H., Horikoshi K.. ( 2003;). Deferribacter desulfuricans sp. nov., a novel sulfur-, nitrate- and arsenate-reducing thermophile isolated from a deep-sea hydrothermal vent. . Int J Syst Evol Microbiol 53:, 839–846. [CrossRef][PubMed]
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
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