1887

Abstract

A novel sulfate-reducing bacterium, strain S28bF, was isolated from tidal flat sediment from Tokyo Bay, Japan. Cells of strain S28bF were rod-shaped (0.5–0.6×1.7–3.8 µm), motile and Gram-stain-negative. For growth, the optimum pH was pH 6.8–7.3 and the optimum temperature was 34–42 °C. Strain S28bF used sulfate and thiosulfate as electron acceptors, but not nitrate. The G+C content of the genomic DNA was 56.6 mol%. The fatty acid profile of strain S28bF was characterized by the presence of anteiso-C and C as the major components. Phylogenetic analyses based on genes for 16S rRNA, the alpha subunit of dissimilatory sulfite reductase () and adenosine-5′-phosphosulfate reductase () revealed that the isolated strain belonged to the class . Its closest relative was DSM 7267 with a 16S rRNA gene sequence similarity of 93.3 %. Two other strains, S28OL1 and S28OL2 were also isolated from the same sediment. These strains were closely related to S28bF with 16S rRNA gene sequence similarities of 99 %, and the same physiological characteristics were shared with strain S28bF. On the basis of phylogenetic and phenotypic characterization, a novel species in a new genus, gen. nov., sp. nov., is proposed to accommodate the strains obtained in this study. The type strain is S28bF ( = NBRC 107166 = DSM 23472).

Funding
This study was supported by the:
  • , Ministry of Education, Culture, Sports, Science and Technology, Japan , (Award 22370005)
  • , Institute for Fermentation, Osaka
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.043356-0
2013-02-01
2020-09-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/2/761.html?itemId=/content/journal/ijsem/10.1099/ijs.0.043356-0&mimeType=html&fmt=ahah

References

  1. Bak F., Widdel F. ( 1986 ). Anaerobic degradation of indolic compounds by sulfate-reducing enrichment cultures, and description of Desulfobacterium indolicum gen. nov., sp. nov. . Arch Microbiol 146, 170176. [CrossRef]
    [Google Scholar]
  2. Cord-Ruwisch R. ( 1985 ). A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. . J Microbiol Methods 4, 3336. [CrossRef]
    [Google Scholar]
  3. Higashioka Y., Kojima H., Fukui M. ( 2011 ). Temperature-dependent differences in community structure of bacteria involved in degradation of petroleum hydrocarbons under sulfate-reducing conditions. . J Appl Microbiol 110, 314322. [CrossRef] [PubMed]
    [Google Scholar]
  4. Katayama-Fujimura Y., Komatsu Y., Kuraishi H., Kaneko T. ( 1984 ). Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. . Agric Biol Chem 48, 31693172. [CrossRef]
    [Google Scholar]
  5. Klein M., Friedrich M., Roger A. J., Hugenholtz P., Fishbain S., Abicht H., Blackall L. L., Stahl D. A., Wagner M. ( 2001 ). Multiple lateral transfers of dissimilatory sulfite reductase genes between major lineages of sulfate-reducing prokaryotes. . J Bacteriol 183, 60286035. [CrossRef] [PubMed]
    [Google Scholar]
  6. Kuever J., Rainey F. A., Widdel F. ( 2005a ). Genus VI. Desufococcus Widdel 1981, 382VP (Effective publication: Widdel 1980, 376). . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, pp. 972974. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  7. Kuever J., Rainey F. A., Widdel F. ( 2005b ). Genus X. Desufosarcina Widdel 1981, 382VP (Effective publication: Widdel 1980, 382). . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, pp. 981984. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  8. Kumar S., Tamura K., Nei M. ( 2004 ). MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. . Brief Bioinform 5, 150163. [CrossRef] [PubMed]
    [Google Scholar]
  9. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . New York:: Wiley;.
    [Google Scholar]
  10. Meyer B., Kuever J. ( 2007 ). Molecular analysis of the diversity of sulfate-reducing and sulfur-oxidizing prokaryotes in the environment, using aprA as functional marker gene. . Appl Environ Microbiol 73, 76647679. [CrossRef] [PubMed]
    [Google Scholar]
  11. Rütters H., Sass H., Cypionka H., Rullkötter J. ( 2001 ). Monoalkylether phospholipids in the sulfate-reducing bacteria Desulfosarcina variabilis and Desulforhabdus amnigenus . . Arch Microbiol 176, 435442. [CrossRef] [PubMed]
    [Google Scholar]
  12. Santillano D., Boetius A., Ramette A. ( 2010 ). Improved dsrA-based terminal restriction fragment length polymorphism analysis of sulfate-reducing bacteria. . Appl Environ Microbiol 76, 53085311. [CrossRef] [PubMed]
    [Google Scholar]
  13. Tabuchi K., Kojima H., Fukui M. ( 2010 ). Seasonal changes in organic matter mineralization in a sublittoral sediment and temperature-driven decoupling of key processes. . Microb Ecol 60, 551560. [CrossRef] [PubMed]
    [Google Scholar]
  14. Widdel F., Bak F. ( 1992 ). Gram-negative mesophilic sulfate-reducing bacteria. . In The Prokaryotes, , 2nd edn., vol. 4, pp. 33523378. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. . New York:: Springer-Verlag;. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.043356-0
Loading
/content/journal/ijsem/10.1099/ijs.0.043356-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error