1887

Abstract

Four strains of a novel heterothallic yeast species were isolated from rotten wood collected in or near the Pilis Mountains in Hungary. The strains produced riboflavin in liquid culture. Analysis of gene sequences for the D1/D2 domains of the LSU nuclear rRNA, as well as analysis of concatenated gene sequences for the D1/D2 nuclear LSU rRNA, mitochondrial SSU rRNA and cytochrome oxidase II placed the novel species in a small clade including only two recognized species, and , in the family Trichomonascaceae. DNA sequence analyses demonstrated that the novel species was distinct from all currently recognized teleomorphic yeast genera. The name gen nov., sp. nov. is proposed to accommodate the novel genus and species. The new genus proposed here can be recognized only from gene sequence analysis, because the characters of its asexual reproduction and ascospore formation are shared by several members of the genera , and . The type and isotype strains of are NCAIM Y.01949 ( = NRRL Y-48781 = CBS 12613) and NCAIM Y.01956 ( = NRRL Y-48782 = CBS 12614), respectively. In view of their close relatedness to , and are transferred to the genus as new combinations in accordance with changes in the International Code of Nomenclature for algae, fungi and plants.

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2012-12-01
2019-10-17
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References

  1. Budavari S.. (editor) ( 1996;). The MERCK Index. . In An Encyclopedia of Chemicals, Drugs and Biologicals, , 12th edn., pp. 1410. Whitehouse Station, NJ:: MERCK & Co.;
    [Google Scholar]
  2. Dlauchy D., Tornai-Lehoczki J., Fülöp L., Péter G.. ( 2003;). Pichia (Komagataella) pseudopastoris sp. nov., a new yeast species from Hungary. . Antonie van Leeuwenhoek 83:, 327–332. [CrossRef][PubMed]
    [Google Scholar]
  3. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  4. Johnson E. A., Echavarri-Erasun C.. ( 2011;). Yeast biotechnology. . In The Yeasts, a Taxonomic Study, , 5th edn., pp. 21–44. Edited by Kurtzman C. P., Fell J. W., Boekhout T... Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
  5. Knapp S., McNeill J., Turland N. J.. ( 2011;). Changes to publication requirements made at the XVIII International Botanical Congress in Melbourne – what does e-publication mean for you?. BMC Evol Biol 11:, 250. [CrossRef][PubMed]
    [Google Scholar]
  6. Kurtzman C. P.. ( 2007a;). Eleven new species of Sugiyamaella and Candida from forest habitats. . FEMS Yeast Res 7:, 1046–1063. [CrossRef][PubMed]
    [Google Scholar]
  7. Kurtzman C. P.. ( 2007b;). New anamorphic yeast species: Candida infanticola sp. nov., Candida polysorbophila sp. nov., Candida transvaalensis sp. nov. and Trigonopsis californica sp. nov.. Antonie van Leeuwenhoek 92:, 221–231. [CrossRef][PubMed]
    [Google Scholar]
  8. Kurtzman C. P.. ( 2007c;). Blastobotrys americana sp. nov., Blastobotrys illinoisensis sp. nov., Blastobotrys malaysiensis sp. nov., Blastobotrys muscicola sp. nov., Blastobotrys peoriensis sp. nov. and Blastobotrys raffinosifermentans sp. nov., novel anamorphic yeast species. . Int J Syst Evol Microbiol 57:, 1154–1162. [CrossRef][PubMed]
    [Google Scholar]
  9. Kurtzman C. P.. ( 2011a;). Discussion of teleomorphic and anamorphic ascomycetous yeasts and yeast-like taxa. . In The Yeasts, a Taxonomic Study, , 5th edn., pp. 293–307. Edited by Kurtzman C. P., Fell J. W., Boekhout T... Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
  10. Kurtzman C. P.. ( 2011b;). Sugiyamaella Kurtzman & Robnett (2007). . In The Yeasts, a Taxonomic Study, , 5th edn., pp. 817–822. Edited by Kurtzman C. P., Fell J. W., Boekhout T... Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
  11. Kurtzman C. P., de Hoog G. S.. ( 2011;). Eremothecium Borzi emend. Kurtzman (1995). . In The Yeasts, a Taxonomic Study, , 5th edn., pp. 405–412. Edited by Kurtzman C. P., Fell J. W., Boekhout T... Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
  12. Kurtzman C. P., Robnett C. J.. ( 1998;). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. . Antonie van Leeuwenhoek 73:, 331–371. [CrossRef][PubMed]
    [Google Scholar]
  13. Kurtzman C. P., Robnett C. J.. ( 2007;). Multigene phylogenetic analysis of the Trichomonascus, Wickerhamiella and Zygoascus yeast clades, and the proposal of Sugiyamaella gen. nov. and 14 new species combinations. . FEMS Yeast Res 7:, 141–151. [CrossRef][PubMed]
    [Google Scholar]
  14. Lachance M. A., Boekhout T., Scorzetti G., Fell J. W., Kurtzman C. P.. ( 2011;). Candida Berkhout (1923). . In The Yeasts, a Taxonomic Study, , 5th edn., pp. 987–1278. Edited by Kurtzman C. P., Fell J. W., Boekhout T... Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
  15. Péter G., Tornai-Lehoczki J., Dlauchy D.. ( 2009;). Candida ogatae sp. nov., an anamorphic member of the Kuraishia clade. . FEMS Yeast Res 9:, 328–333. [CrossRef][PubMed]
    [Google Scholar]
  16. Péter G., Dlauchy D., Tornai-Lehoczki J., Suzuki M., Kurtzman C. P.. ( 2011;). Spencermartinsiella europaea gen. nov., sp. nov., a new member of the family Trichomonascaceae. . Int J Syst Evol Microbiol 61:, 993–1000. [CrossRef][PubMed]
    [Google Scholar]
  17. Smith M. Th., de Hoog G. S., Malloch D., Kurtzman C. P.. ( 2011;). Trichomonascus H.S. Jackson emend. Kurtzman & Robnett (2007). . In The Yeasts, a Taxonomic Study, , 5th edn., pp. 875–881. Edited by Kurtzman C. P., Fell J. W., Boekhout T... Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
  18. Stahmann K.-P., Revuelta J. L., Seulberger H.. ( 2000;). Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production. . Appl Microbiol Biotechnol 53:, 509–516. [CrossRef][PubMed]
    [Google Scholar]
  19. Swofford D. L.. ( 1998;). Phylogenetic analysis using parsimony (paup), version 4. Sunderland, MA: Sinauer Associates.
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  21. Yarrow D.. ( 1998;). Methods for the isolation, maintenance and identification of yeasts. . In The Yeasts, a Taxonomic Study, , 4th edn., pp. 77–100. Edited by Kurtzman C. P., Fell J. W... Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
  22. Zhang Z., Schwartz S., Wagner L., Miller W.. ( 2000;). A greedy algorithm for aligning DNA sequences. . J Comput Biol 7:, 203–214. [CrossRef][PubMed]
    [Google Scholar]
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