1887

Abstract

A strictly anaerobic Gram-stain-variable but positive by structure, non-spore-forming bacterium designated bacterium ACC2 strain DSM 24645 was isolated from human subgingival dental plaque. Bacterial cells were 4–40 µm long non-motile rods, often swollen and forming curved filaments up to 200 µm. Cells contained intracellular, poorly crystalline, nanometre-sized iron- and sulfur-rich particles. The micro-organism was able to grow on yeast extract, trypticase peptone, milk, some sugars and organic acids. The major metabolic end-products of glucose fermentation were butyrate, lactate, isovalerate and acetate. The growth temperature and pH ranges were 30–42 °C and 4.9–7.5, respectively. Major fatty acids were C, C DMA (dimethyl aldehyde), C, Cω7 DMA. The whole-cell hydrolysate contained -diaminopimelic acid, indicating peptidoglycan type A1γ. The DNA G+C content was calculated to be 55.05 mol% from the whole-genome sequence and 55.3 mol% as determined by HPLC. There were no predicted genes responsible for biosynthesis of respiratory lipoquinones, mycolic acids and lipopolysaccharides. Genes associated with synthesis of teichoic and lipoteichoic acids, diaminopimelic acid, polar lipids and polyamines were present. According to the 16S rRNA gene sequence phylogeny, strain DSM 24645 formed, together with several uncultured oral clones, a separate branch within the family , with the highest sequence similarity to the type strain of at 94.2 %. Based on distinct phenotypic and genotypic characteristics, we suggest that strain DSM 24645 represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is DSM 24645 ( = HM-480; deposited in BEI Resources, an NIH collection managed by the ATCC).

Funding
This study was supported by the:
  • NIH (Award 3 R21 DE018026-02S1 and 1RC1DE020707-01)
  • NSF (Award EF-1002148 and EAR-0920718)
  • US Department of Energy, Basic Energy Sciences, Materials Sciences and Engineering Division (Award DE-AC02-07CH11358)
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2013-04-01
2021-06-24
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