1887

Abstract

Bacterial strain 14-2A, isolated from a long-term DDT-contaminated soil in China, was characterized by using a polyphasic approach to clarify its taxonomic position. Strain 14-2A was found to be Gram-negative, aerobic, non-spore-forming, non-motile, non-flagellated and rod-shaped. The new isolate was able to grow at 4–42 °C, pH 6.0–9.0 and with 0–5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the family . The 16S rRNA gene sequence of strain 14-2A showed the highest similarity with TBF2/20.2 (99.4 %), followed by DC-186 (93.8 %), Gsoil 060 (93.6 %), Jip13 (93.1 %), Gsoil 034 (92.8 %) and AW-6 (89.6 %). The DNA–DNA hybridization value between strains 14-2A and TBF2/20.2 was 34.45±2.11 %. Strain 14-2A contained phosphatidylethanolamine, phosphatidylmonomethylethanolamine, aminophospholipid and phosphatidylinositol mannoside as the major polar lipids. The DNA G+C content was 41.2 mol%. MK-7 is the major isoprenoid quinone. Summed feature 3 (Cω7 and/or Cω6), iso-C and iso-C 3-OH are the major fatty acids. The phenotypic and chemotaxonomic data confirmed the affiliation of strain 14-2A to the genus . On the basis of the phylogenetic and phenotypic characteristics, and chemotaxonomic data, strain 14-2A is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is 14-2A ( = KCTC 23098 = CCTCC AB 2010105).

Funding
This study was supported by the:
  • , National Basic Research Program of China , (Award 2010CB833801)
  • , Chinese National Natural Science Foundation , (Award 31070100)
  • , Fundamental Research Fund
  • , Environmental and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science , (Award 2010hzszdzx001)
  • , National Undergraduate Innovative Test Program , (Award 101030717)

Erratum

This article contains a correction applying to the following content:
sp. nov., isolated from DDT-contaminated soil
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.042416-0
2013-03-01
2020-07-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/3/1083.html?itemId=/content/journal/ijsem/10.1099/ijs.0.042416-0&mimeType=html&fmt=ahah

References

  1. Buck J. D. ( 1982 ). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. . Appl Environ Microbiol 44, 992993.[PubMed]
    [Google Scholar]
  2. Collins M. D., Jones D. ( 1980 ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. . J Appl Bacteriol 48, 459470. [CrossRef]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [CrossRef] [PubMed]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [CrossRef]
    [Google Scholar]
  5. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  7. Felsenstein J. ( 2002 ). phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [CrossRef]
    [Google Scholar]
  9. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kluge A. G., Farris F. S. ( 1969 ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18, 132. [CrossRef]
    [Google Scholar]
  12. Kovacs N. ( 1956 ). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178, 703704. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kroppenstedt R. M. ( 1982 ). Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5, 23592367. [CrossRef]
    [Google Scholar]
  14. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  15. Li R., Zheng J., Wang R., Song Y., Chen Q., Yang X., Li S., Jiang J. ( 2010 ). Biochemical degradation pathway of dimethoate by Paracoccus sp. Lgjj-3 isolated from treatment wastewater. . Int Biodeterior Biodegradation 64, 5157. [CrossRef]
    [Google Scholar]
  16. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [CrossRef]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  18. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [CrossRef]
    [Google Scholar]
  19. Ntougias S., Fasseas C. , Zervakis G.I. ( 2007 ). Olivibacter sitiensis gen. nov., sp. nov., isolated from alkaline olive-oil mill wastes in the region of Sitia, Crete. . Int J Syst Evol Microbiol 57, 398404. [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  21. Sambrook J., Russell D. W. ( 2001 ). Molecular cloning: a Laboratory Manual, , 3rd ed.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  22. Steyn P. L., Segers P., Vancanneyt M., Sandra P., Kersters K., Joubert J. J. ( 1998 ). Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. proposal of the family Sphingobacteriaceae fam. nov.. Int J Syst Bacteriol 48, 165177. [CrossRef] [PubMed]
    [Google Scholar]
  23. Szabó I., Szoboszlay S., Kriszt B., Háhn J., Harkai P., Baka E., Táncsics A., Kaszab E., Privler Z., Kukolya J. ( 2011 ). Olivibacter oleidegradans sp. nov., a hydrocarbon-degrading bacterium isolated from a biofilter clean-up facility on a hydrocarbon-contaminated site. . Int J Syst Evol Microbiol 61, 28612865. [CrossRef] [PubMed]
    [Google Scholar]
  24. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetic analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [CrossRef] [PubMed]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  26. Wang L., Ten L. N., Lee H. G., Im W. T., Lee S. T. ( 2008 ). Olivibacter soli sp. nov., Olivibacter ginsengisoli sp. nov. and Olivibacter terrae sp. nov., from soil of a ginseng field and compost in South Korea. . Int J Syst Evol Microbiol 58, 11231127. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.042416-0
Loading
/content/journal/ijsem/10.1099/ijs.0.042416-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error