1887

Abstract

Bacterial strain 14-2A, isolated from a long-term DDT-contaminated soil in China, was characterized by using a polyphasic approach to clarify its taxonomic position. Strain 14-2A was found to be Gram-negative, aerobic, non-spore-forming, non-motile, non-flagellated and rod-shaped. The new isolate was able to grow at 4–42 °C, pH 6.0–9.0 and with 0–5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the family . The 16S rRNA gene sequence of strain 14-2A showed the highest similarity with TBF2/20.2 (99.4 %), followed by DC-186 (93.8 %), Gsoil 060 (93.6 %), Jip13 (93.1 %), Gsoil 034 (92.8 %) and AW-6 (89.6 %). The DNA–DNA hybridization value between strains 14-2A and TBF2/20.2 was 34.45±2.11 %. Strain 14-2A contained phosphatidylethanolamine, phosphatidylmonomethylethanolamine, aminophospholipid and phosphatidylinositol mannoside as the major polar lipids. The DNA G+C content was 41.2 mol%. MK-7 is the major isoprenoid quinone. Summed feature 3 (Cω7 and/or Cω6), iso-C and iso-C 3-OH are the major fatty acids. The phenotypic and chemotaxonomic data confirmed the affiliation of strain 14-2A to the genus . On the basis of the phylogenetic and phenotypic characteristics, and chemotaxonomic data, strain 14-2A is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is 14-2A ( = KCTC 23098 = CCTCC AB 2010105).

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2013-03-01
2019-10-18
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