1887

Abstract

A taxonomic study was carried out on a novel bacterium, designated strain 3-C-1, which was isolated from a crude-oil-degrading consortium produced by enrichment of a sample of surface seawater collected near Xiamen Island in China. Cells of the novel strain were Gram-reaction-negative, oxidase- and catalase-positive, rod-shaped, motile and amphitrichous. Growth was observed with 0–12 % (w/v) NaCl and at temperatures of 10–42 °C. The novel strain was incapable of nitrate reduction. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-C-1 belonged to the genus and was most closely related to CEE_131 (with sequence similarities of 96.5–96.8 %). The gene sequence of strain 3-C-1 was also similar to that of CEE_131 (89.4 % sequence similarity). The results of PCR-based comparison of repetitive extragenic palindromic elements (Rep-PCR fingerprinting) allowed strain 3-C-1 to be distinguished from CEE_131. The principal fatty acids of the novel strain were C, C 3-OH, summed feature 3 (Cω6 and/or Cω7) and summed feature 8 (Cω6 and/or Cω7). The G+C content of the novel strain’s genomic DNA was 61.3 mol%. The combined genotypic and phenotypic data indicate that strain 3-C-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 3-C-1 ( = CCTCC AB 209060  = LMG 25226  = MCCC 1A01354).

Funding
This study was supported by the:
  • , State Oceanic Administration, China , (Award 201005032)
  • , State Oceanic Administration, China , (Award 2010137)
  • , National Infrastructure of Natural Resources for Science and Technology Program of China , (Award NIMR-2011-9)
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2013-03-01
2020-08-05
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