1887

Abstract

A light-yellow-pigmented bacterial strain designated JS43 was isolated from a freshwater spring in Taiwan and was characterized using a polyphasic taxonomic approach. Cells of strain JS43 were Gram-negative-staining, facultatively anaerobic, rod-shaped, non-motile and non-spore-forming. Growth occurred at 10–30 °C (optimum, 25 °C), at pH 7.0–10.0 (optimum, pH 7.0–8.0) and with 0–0.5 % (w/v) NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain JS43 was a member of the family of the class . Sequence similarities to type strains of the genera and were between 94.6 and 98.1 %. The highest sequence similarity was found with DSM 3857. Strain JS43 contained Cω7 as the predominant fatty acid (74.4 %). The major isoprenoid quinone was Q-10 and the DNA G+C content was 69.3 mol%. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two uncharacterized aminolipids and three uncharacterized phospholipids. The DNA–DNA relatedness values of strain JS43 with respect to the phylogenetically related DSM 3857, A1-9 and DCA-1 were less than 70 %. The taxonomic relationship between members of the genera and was clarified by means of a direct experimental comparison. Based on phylogenetic, chemotaxonomic and phenotypic data, we propose that all species currently classified in the genus should be transferred to the genus. The following new combinations are proposed: is reclassified as comb. nov. (type strain, AST4 = DSM 15620 = JCM 11959 = NBRC 100046), as comb. nov. (type strain, JA139 = CCUG 53722 = DSM 18774 = JCM 14338), as nom. nov. (type strain, A1-9 = CGMCC 1.7029 = NBRC 104254), as comb. nov. (type strain, DCA-1 = CGMCC 1.7745 = DSM 21823) and as comb. nov. (type strain, Y12 = CCTCC AB 2010218 = KCTC 23298). Emended descriptions of the genus and of are also presented. Strain JS43 could be distinguished from recognized species of the genera and . It is suggested, on the basis of genotypic and phenotypic characteristics, that strain JS43 ( = BCRC 80082 = LMG 25376) represents a novel species for which the name is proposed.

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2013-02-01
2024-12-14
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