1887

Abstract

A novel Gram-stain-negative, facultatively anaerobic, non-motile and coccus-shaped bacterium, strain C7, was isolated from the gut of the butterfly . Strain C7 grew optimally at 20–25 °C, at pH 7–8 and with 1 % (w/v) NaCl. The strain was negative for oxidase activity but positive for catalase activity. The 16S rRNA gene sequences of strain C7 and CN3 shared 96.8 % similarity. The major fatty acids identified were C, C, Cω7 and summed feature 2 (comprising C 3-OH/iso-C). The major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids of strain C7 were phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid and two unidentified aminophospholipids. The G+C content of the genomic DNA extracted from strain C7 was 32.1 mol%. Taken together, the phenotypic, genotypic and phylogenetic analyses indicate that strain C7 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is C7 ( = KACC 16544 = JCM 18050). An emended description of the genus is provided.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.041871-0
2013-05-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/5/1766.html?itemId=/content/journal/ijsem/10.1099/ijs.0.041871-0&mimeType=html&fmt=ahah

References

  1. Baker G. C. , Smith J. J. , Cowan D. A. . ( 2003; ). Review and re-analysis of domain-specific 16S primers. . J Microbiol Methods 55:, 541–555. [CrossRef] [PubMed]
    [Google Scholar]
  2. Chun J. , Lee J. H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  3. Collins M. D. , Jones D. . ( 1981a; ). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. . Microbiol Rev 45:, 316–354.[PubMed]
    [Google Scholar]
  4. Collins M. D. , Jones D. . ( 1981b; ). A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. . J Appl Bacteriol 51:, 129–134. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  6. Gonzalez J. M. , Saiz-Jimenez C. . ( 2002; ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4:, 770–773. [CrossRef] [PubMed]
    [Google Scholar]
  7. Kluge A. G. , Farris F. S. . ( 1969; ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  8. MIDI (1999). Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE: MIDI, Inc.
  9. Rochelle P. A. , Fry J. C. , Parkes R. J. , Weightman A. J. . ( 1992; ). DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities. . FEMS Microbiol Lett 79:, 59–65.[PubMed] [CrossRef]
    [Google Scholar]
  10. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  11. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  12. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  13. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  14. Tindall B. J. . ( 1990; ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  15. Tittsler R. P. , Sandholzer L. A. . ( 1936; ). The use of semi-solid agar for the detection of bacterial motility. . J Bacteriol 31:, 575–580.[PubMed]
    [Google Scholar]
  16. Volkmann M. , Skiebe E. , Kerrinnes T. , Faber F. , Lepka D. , Pfeifer Y. , Holland G. , Bannert N. , Wilharm G. . ( 2010; ). Orbus hercynius gen. nov., sp. nov., isolated from faeces of wild boar, is most closely related to members of the orders ‘Enterobacteriales’ and Pasteurellales . . Int J Syst Evol Microbiol 60:, 2601–2605. [CrossRef] [PubMed]
    [Google Scholar]
  17. Weisburg W. G. , Barns S. M. , Pelletier D. A. , Lane D. J. . ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  18. Xin H. , Itoh T. , Zhou P. , Suzuki K. , Kamekura M. , Nakase T. . ( 2000; ). Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. . Int J Syst Evol Microbiol 50:, 1297–1303. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.041871-0
Loading
/content/journal/ijsem/10.1099/ijs.0.041871-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error