1887

Abstract

The taxonomic properties of strain DC2a-G7, a Gram-negative, ovoid to rod-shaped, gellan gum-lysing bacterium, were examined. The 16S rRNA gene sequence similarity showed that DC2a-G7 is a member of the phylum and the closest type strain of a species with a validly published name is DSM 4136, with a sequence similarity of 91.2 %. In addition to this similarity value lower than 95 %, the absence of prostheca, the orangey-red colony colour and the compositions of the major menaquinones and polar lipids also supported the differentiation of this bacterium from the genus . Here, we propose the name gen. nov., sp. nov. for the isolate. The type strain of is DC2a-G7 ( = NBRC 108606 = DSM 25532).

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2013-06-01
2019-12-13
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References

  1. Ashida N. , Ishii S. , Hayano S. , Tago K. , Tsuji T. , Yoshimura Y. , Otsuka S. , Senoo K. . ( 2010; ). Isolation of functional single cells from environments using a micromanipulator: application to study denitrifying bacteria. . Appl Microbiol Biotechnol 85:, 1211–1217. [CrossRef] [PubMed]
    [Google Scholar]
  2. Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  3. Hamada M. , Iino T. , Iwami T. , Harayama S. , Tamura T. , Suzuki K. . ( 2010; ). Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov.. J Gen Appl Microbiol 56:, 427–436. [CrossRef] [PubMed]
    [Google Scholar]
  4. Hedlund B. P. . ( 2010; ). Phylum XXIII. Verrucomicrobia phyl. nov. . In Bergey's Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 795–841. Edited by Krieg N. R. , Staley J. T. , Brown D. R. , Hedlund B. P. , Paster B. J. . . New York:: Springer;.
    [Google Scholar]
  5. Hedlund B. P. , Gosink J. J. , Staley J. T. . ( 1997; ). Verrucomicrobia div. nov., a new division of the bacteria containing three new species of Prosthecobacter . . Antonie van Leeuwenhoek 72:, 29–38. [CrossRef] [PubMed]
    [Google Scholar]
  6. Huang X. , Miller W. . ( 1991; ). A time-efficient, linear-space local similarity algorithm. . Adv Appl Math 12:, 337–357. [CrossRef]
    [Google Scholar]
  7. Hugenholtz P. , Goebel B. M. , Pace N. R. . ( 1998; ). Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. . J Bacteriol 180:, 4765–4774.[PubMed]
    [Google Scholar]
  8. Ichimura, T. (1971). Sexual cell division and conjugation-papilla formation in sexual reproduction of Closterium strigosum. In Proceedings of the 7th International Seaweed Symposium, pp. 208–214. Edited by K. Nishizawa. Tokyo: University of Tokyo Press.
  9. Janssen P. H. , Schuhmann A. , Mörschel E. , Rainey F. A. . ( 1997; ). Novel anaerobic ultramicrobacteria belonging to the Verrucomicrobiales lineage of bacterial descent isolated by dilution culture from anoxic rice paddy soil. . Appl Environ Microbiol 63:, 1382–1388.[PubMed]
    [Google Scholar]
  10. Janssen P. H. , Yates P. S. , Grinton B. E. , Taylor P. M. , Sait M. . ( 2002; ). Improved culturability of soil bacteria and isolation in pure culture of novel members of the divisions Acidobacteria, Actinobacteria, Proteobacteria, and Verrucomicrobia . . Appl Environ Microbiol 68:, 2391–2396. [CrossRef] [PubMed]
    [Google Scholar]
  11. Katsuta A. , Adachi K. , Matsuda S. , Shizuri Y. , Kasai H. . ( 2005; ). Ferrimonas marina sp. nov.. Int J Syst Evol Microbiol 55:, 1851–1855. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kumar S. , Nei M. , Dudley J. , Tamura K. . ( 2008; ). MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. . Brief Bioinform 9:, 299–306. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–147. Edited by Stackebrandt E. , Goodfellow M. . . New York:: John Wiley & Sons;.
    [Google Scholar]
  14. Martinez-Romero E. , Caballero-Mellado J. . ( 1996; ). Rhizobium phylogenies and bacterial genetic diversity. . Crit Rev Plant Sci 15:, 113–140.[CrossRef]
    [Google Scholar]
  15. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  16. Pruesse E. , Quast C. , Knittel K. , Fuchs B. M. , Ludwig W. , Peplies J. , Glöckner F. O. . ( 2007; ). SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. . Nucleic Acids Res 35:, 7188–7196. [CrossRef] [PubMed]
    [Google Scholar]
  17. Saito T. , Ishii S. , Otsuka S. , Nishiyama M. , Senoo K. . ( 2008; ). Identification of novel betaproteobacteria in a succinate-assimilating population in denitrifying rice paddy soil by using stable isotope probing. . Microbes Environ 23:, 192–200. [CrossRef] [PubMed]
    [Google Scholar]
  18. Sangwan P. , Chen X. , Hugenholtz P. , Janssen P. H. . ( 2004; ). Chthoniobacter flavus gen. nov., sp. nov., the first pure-culture representative of subdivision two, Spartobacteria classis nov., of the phylum Verrucomicrobia . . Appl Environ Microbiol 70:, 5875–5881. [CrossRef] [PubMed]
    [Google Scholar]
  19. Sangwan P. , Kovac S. , Davis K. E. R. , Sait M. , Janssen P. H. . ( 2005; ). Detection and cultivation of soil Verrucomicrobia . . Appl Environ Microbiol 71:, 8402–8410. [CrossRef] [PubMed]
    [Google Scholar]
  20. Schlesner H. . ( 1987; ). Verrucomicrobium spinosum gen. nov., sp. nov. a fimbriated prosthecate bacterium. . Syst Appl Microbiol 10:, 54–56. [CrossRef]
    [Google Scholar]
  21. Seviour E. M. , Williams C. , DeGrey B. , Soddell J. A. , Seviour K. C. , Lindrea K. C. . ( 1994; ). Studies on filamentous bacteria from Australian activated sludge plants. . Water Res 28:, 2335–2342. [CrossRef]
    [Google Scholar]
  22. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: A place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  23. Staley J. T. , Mandel M. . ( 1973; ). Deoxyribonucleic acid base composition of Prosthecomicrobium and Ancalomicrobium strains. . Int J Syst Bacteriol 23:, 271–273. [CrossRef]
    [Google Scholar]
  24. Stevenson B. S. , Eichorst S. A. , Wertz J. T. , Schmidt T. M. , Breznak J. A. . ( 2004; ). New strategies for cultivation and detection of previously uncultured microbes. . Appl Environ Microbiol 70:, 4748–4755. [CrossRef] [PubMed]
    [Google Scholar]
  25. Takeda M. , Yoneya A. , Miyazaki Y. , Kondo K. , Makita H. , Kondoh M. , Suzuki I. , Koizumi J. . ( 2008; ). Prosthecobacter fluviatilis sp. nov., which lacks the bacterial tubulin btubA and btubB genes. . Int J Syst Evol Microbiol 58:, 1561–1565. [CrossRef] [PubMed]
    [Google Scholar]
  26. Thi Vu H. , Otsuka S. , Ueda H. , Senoo K. . ( 2010; ). Cocultivated bacteria can increase or decrease the culture lifetime of Chlorella vulgaris . . J Gen Appl Microbiol 56:, 413–418. [CrossRef] [PubMed]
    [Google Scholar]
  27. Ueda H. , Otsuka S. , Senoo K. . ( 2010; ). Bacterial communities constructed in artificial consortia of bacteria and Chlorella vulgaris . . Microbes Environ 25:, 36–40. [CrossRef] [PubMed]
    [Google Scholar]
  28. Yoon J. , Yasumoto-Hirose M. , Matsuo Y. , Nozawa M. , Matsuda S. , Kasai H. , Yokota A. . ( 2007a; ). Pelagicoccus mobilis gen. nov., sp. nov., Pelagicoccus albus sp. nov. and Pelagicoccus litoralis sp. nov., three novel members of subdivision 4 within the phylum ‘Verrucomicrobia’, isolated from seawater by in situ cultivation. . Int J Syst Evol Microbiol 57:, 1377–1385. [CrossRef] [PubMed]
    [Google Scholar]
  29. Yoon J. , Matsuo Y. , Matsuda S. , Adachi K. , Kasai H. , Yokota A. . ( 2007b; ). Rubritalea spongiae sp. nov. and Rubritalea tangerina sp. nov., two carotenoid- and squalene-producing marine bacteria of the family Verrucomicrobiaceae within the phylum ‘Verrucomicrobia’, isolated from marine animals. . Int J Syst Evol Microbiol 57:, 2337–2343. [CrossRef] [PubMed]
    [Google Scholar]
  30. Yoon J. , Matsuo Y. , Adachi K. , Nozawa M. , Matsuda S. , Kasai H. , Yokota A. . ( 2008; ). Description of Persicirhabdus sediminis gen. nov., sp. nov., Roseibacillus ishigakijimensis gen. nov., sp. nov., Roseibacillus ponti sp. nov., Roseibacillus persicicus sp. nov., Luteolibacter pohnpeiensis gen. nov., sp. nov. and Luteolibacter algae sp. nov., six marine members of the phylum ‘Verrucomicrobia’, and emended descriptions of the class Verrucomicrobiae, the order Verrucomicrobiales and the family Verrucomicrobiaceae . . Int J Syst Evol Microbiol 58:, 998–1007. [CrossRef] [PubMed]
    [Google Scholar]
  31. Yoon J. , Matsuo Y. , Matsuda S. , Kasai H. , Yokota A. . ( 2010; ). Cerasicoccus maritimus sp. nov. and Cerasicoccus frondis sp. nov., two peptidoglycan-less marine verrucomicrobial species, and description of Verrucomicrobia phyl. nov., nom. rev.. J Gen Appl Microbiol 56:, 213–222. [CrossRef] [PubMed]
    [Google Scholar]
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