1887

Abstract

An actinomycete strain, designated Sco-B23, was isolated from a brown-coloured layer of scoria collected near Darangshi Oreum (a small mountain) in Jeju, Republic of Korea. Cells of the organism were Gram-positive, non-endospore-forming, non-motile cocci and grew at 20–35 °C, at pH 5.1–11.1 and with 0–1 % (w/v) NaCl. Colonies were circular, translucent and moderate yellow in colour with entire margins. On the basis of 16S rRNA gene sequence analysis, the isolate belonged to the genus and formed a monophyletic line between a cluster and a cluster. Levels of 16S rRNA gene sequence similarity between strain Sco-B23 and the type strains of species were: (98.8 %), (98.7 %), (97.7 %), (97.7 %), (96.9 %) and (96.5 %). Chemotaxonomic analyses showed that the isolate possessed -diaminopimelic acid as the diamino acid of the peptidoglycan, MK-8(H) as the predominant menaquinone, a polar lipid profile including diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unknown phospholipid and two unknown lipids, predominant fatty acids represented by iso-C, C 9, iso-C and iso-C, and DNA G+C content of 69.7 mol%. DNA–DNA relatedness between strain Sco-B23 and the close relatives within the genus was below 18.1 %. On the basis of phenotypic features and DNA–DNA hybridization data, strain Sco-B23 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Sco-B23 ( = KCTC 19807  = KACC 15111  = NBRC 107918).

Funding
This study was supported by the:
  • , 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science & Technology, Republic of Korea
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2013-03-01
2020-12-02
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