1887

Abstract

X-disease is one of the most serious diseases known in peach (). Based on RFLP analysis of 16S rRNA gene sequences, peach X-disease phytoplasma strains from eastern and western United States and eastern Canada were classified in 16S rRNA gene RFLP group 16SrIII, subgroup A. Phylogenetic analyses of 16S rRNA gene sequences revealed that the X-disease phytoplasma strains formed a distinct subclade within the phytoplasma clade, supporting the hypothesis that they represented a lineage distinct from those of previously described ‘ Phytoplasma ’ species. Nucleotide sequence alignments revealed that all studied X-disease phytoplasma strains shared less than 97.5 % 16S rRNA gene sequence similarity with previously described ‘ Phytoplasma ’ species. Based on unique properties of the DNA, we propose recognition of X-disease phytoplasma strain PX11CT1 as representative of a novel taxon, ‘ Phytoplasma pruni’. Results from nucleotide and phylogenetic analyses of and ribosomal protein (rp) gene sequences provided additional molecular markers of the ‘. Phytoplasma pruni’ lineage. We propose that the term ‘ Phytoplasma pruni’ be applied to phytoplasma strains whose 16S rRNA gene sequences contain the oligonucleotide sequences of unique regions that are designated in the formally published description of the taxon. Such strains include X-disease phytoplasma and - within the tolerance of a single base difference in one unique sequence - peach rosette, peach red suture, and little peach phytoplasmas. Although not employed for taxon delineation in this work, we further propose that , rp, and other genetic loci from the reference strain of a taxon, and where possible oligonucleotide sequences of unique regions of those genes that distinguish taxa within a given 16Sr group, be incorporated in emended descriptions and as part of future descriptions of ‘ Phytoplasma ’ taxa.

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2013-02-01
2019-10-23
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References

  1. Allen W. R. , Davidson T. R. . ( 1978; ). Dosage response and duration of symptom remission in X-diseased peach trees treated with oxytetracycline-HCl in Ontario. . Plant Disease Reporter 62:, 311–313.
    [Google Scholar]
  2. Amin P. W. , Jensen D. D. . ( 1971; ). Effects of tetracycline on the transmission and pathogenicity of the western X disease agent in its insect and plant hosts. . Phytopathology 61:, 696–702. [CrossRef]
    [Google Scholar]
  3. Cooley D. R. , Tattar T. A. , Schieffer J. T. . ( 1992; ). Treatment of X-disease of peaches using oxytetracycline microinjection capsules. . HortScience 27:, 235–237.
    [Google Scholar]
  4. Davis R. E. , Sinclair W. A. . ( 1998; ). Phytoplasma identity and disease etiology. . Phytopathology 88:, 1372–1376. [CrossRef] [PubMed]
    [Google Scholar]
  5. Davis R. E. , Jomantiene R. , Kalvelyte A. , Dally E. L. . ( 2003; ). Differential amplification of sequence heterogeneous ribosomal RNA genes and classification of the ‘Fragaria multicipita’ phytoplasma. . Microbiol Res 158:, 229–236. [CrossRef] [PubMed]
    [Google Scholar]
  6. Davis R. E. , Jomantiene R. , Zhao Y. . ( 2005; ). Lineage-specific decay of folate biosynthesis genes suggests ongoing host adaptation in phytoplasmas. . DNA Cell Biol 24:, 832–840. [CrossRef] [PubMed]
    [Google Scholar]
  7. Davis R. E. , Zhao Y. , Dally E. L. , Jomantiene R. , Lee I. M. , Wei W. , Kitajima E. W. . ( 2012; ). Candidatus Phytoplasma sudamericanum’, a novel taxon, and strain PassWB-Br4, a new subgroup 16SrIII-V phytoplasma, from diseased passion fruit (Passiflora edulis f. flavicarpa Deg.). . Int J Syst Evol Microbiol 62:, 984–989. [CrossRef] [PubMed]
    [Google Scholar]
  8. Douglas S. M. . ( 1986; ). Detection of mycoplasma-like organisms in peach and chokecherry with X-disease by fluorescence microscopy. . Phytopathology 76:, 784–787. [CrossRef]
    [Google Scholar]
  9. Douglas S. M. , McClure M. S. . ( 1988; ). New integrated approach for controlling X-disease of stone fruits. . In Connecticut Agricultural Experiment Station Bulletin No 854, pp. 1–10. New Haven:: Connecticut Agricultural Experiment Station;. [CrossRef]
    [Google Scholar]
  10. Granett A. L. , Gilmer R. M. . ( 1971; ). Mycoplasmas associated with X-disease in various Prunus species. . Phytopathology 61:, 1036–1037. [CrossRef]
    [Google Scholar]
  11. Green M. J. , Thompson D. A. , MacKenzie D. J. . ( 1999; ). Easy and efficient DNA extraction from woody plants for the detection of phytoplasmas by polymerase chain reaction. . Plant Dis 83:, 482–485. [CrossRef]
    [Google Scholar]
  12. Gundersen D. E. , Lee I.-M. , Rehner S. A. , Davis R. E. , Kingsbury D. T. . ( 1994; ). Phylogeny of mycoplasmalike organisms (phytoplasmas): a basis for their classification. . J Bacteriol 176:, 5244–5254.[PubMed]
    [Google Scholar]
  13. IRPCM ( 2004; ). Candidatus Phytoplasma’, a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects. . Int J Syst Evol Microbiol 54:, 1243–1255. [CrossRef] [PubMed]
    [Google Scholar]
  14. Jones A. L. , Hooper G. R. , Rosenberger D. A. . ( 1974; ). Association of mycoplasmalike bodies with little peach and X-disease. . Phytopathology 64:, 755–756. [CrossRef]
    [Google Scholar]
  15. Kirkpatrick B. C. , Fisher G. A. , Fraser J. D. , Purcell A. H. . ( 1990; ). Epidemiology and phylogenetic studies on western X-disease mycoplasma-like organisms. . In Recent Advances in Mycoplasmology. Proceedings of the 7th Congress of the International Organization for Mycoplasmology, Baden near Vienna, 1988, pp. 288–297. Stuttgart:: Gustav Fischer Verlag;.
    [Google Scholar]
  16. Lacy G. H. . ( 1982; ). Peach X-disease: Treatment site damage and yield response following antibiotic infusion. . Plant Dis 66:, 1129–1133. [CrossRef]
    [Google Scholar]
  17. Lacy G. H. , McIntyre J. L. , Walton G. S. , Dodds J. A. . ( 1980; ). Rapid method for and effects of infusing trees with concentrated oxytetracycline-HCl solutions for pear decline control. . Can J Plant Pathol 2:, 96–101. [CrossRef]
    [Google Scholar]
  18. Larsen K. J. , Whalen M. E. . ( 1988; ). Dispersal of Paraphlopsius irroratus (Say) (Homoptera: Cicadellidae) in peach and cherry orchards. . Environ Entomol 17:, 842–851.[CrossRef]
    [Google Scholar]
  19. Lee I. M. , Davis R. E. , Hiruki C. . ( 1991; ). Genetic interrelatedness among clover proliferation mycoplasmalike organisms (MLOs) and other MLOs investigated by nucleic acid hybridization and restriction fragment length polymorphism analyses. . Appl Environ Microbiol 57:, 3565–3569.[PubMed]
    [Google Scholar]
  20. Lee I.-M. , Gundersen D. E. , Davis R. E. , Chiykowski L. N. . ( 1992; ). Identification and analysis of a genomic strain cluster of mycoplasmalike organisms associated with Canadian peach (eastern) X disease, western X disease, and clover yellow edge. . J Bacteriol 174:, 6694–6698.[PubMed]
    [Google Scholar]
  21. Lee I.-M. , Gundersen-Rindal D. E. , Davis R. E. , Bartoszk I. M. . ( 1998; ). Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. . Phytopathology 83:, 834–842. [CrossRef]
    [Google Scholar]
  22. Lee I.-M. , Gundersen-Rindal D. E. , Davis R. E. , Bottner K. D. , Marcone C. , Seemüller E. . ( 2004; ). Candidatus Phytoplasma asteris”, a novel phytoplasma taxon associated with aster yellows and related diseases. . Int J Syst Evol Microbiol 54:, 1037–1048. [CrossRef] [PubMed]
    [Google Scholar]
  23. Lee I.-M. , Bottner-Parker K. D. , Zhao Y. , Davis R. E. , Harrison N. A. . ( 2010; ). Phylogenetic analysis and delineation of phytoplasmas based on secY gene sequences. . Int J Syst Evol Microbiol 60:, 2887–2897. [CrossRef] [PubMed]
    [Google Scholar]
  24. Lukens R. J. , Miller P. M. , Walton G. S. , Hitchcock S. W. . ( 1971; ). Incidence of X-disease of peach and eradication of chokecherry. . Plant Disease Reporter 55:, 645–647.
    [Google Scholar]
  25. MacBeath J. H. , Nyland G. , Spurr A. R. . ( 1972; ). Morphology of mycoplasma-like bodies associated with peach X-disease in Prunus persica . . Phytopathology 62:, 935–937. [CrossRef]
    [Google Scholar]
  26. Marcone C. , Neimark H. , Ragozzino A. , Lauer U. , Seemüller E. . ( 1999; ). Chromosome sizes of phytoplasmas composing major phylogenetic groups and subgroups. . Phytopathology 89:, 805–810. [CrossRef] [PubMed]
    [Google Scholar]
  27. Martini M. , Lee I. M. , Bottner K. D. , Zhao Y. , Botti S. , Bertaccini A. , Harrison N. A. , Carraro L. , Marcone C. . & other authors ( 2007; ). Ribosomal protein gene-based phylogeny for finer differentiation and classification of phytoplasmas. . Int J Syst Evol Microbiol 57:, 2037–2051. [CrossRef] [PubMed]
    [Google Scholar]
  28. Martini M. , Marcone C. , Mitrović J. , Maixner M. , Delić D. , Myrta A. , Ermacora P. , Bertaccini A. , Duduk B. . ( 2012; ). Candidatus Phytoplasma convolvuli”, a new phytoplasma taxon associated with bindweed yellows in four European countries. . Int J Syst Evol Microbiol 62:, 2910–2915. [CrossRef]
    [Google Scholar]
  29. McClure M. S. . ( 1980; ). Spatial and seasonal distribution of leafhopper vectors of peach X-disease in Connecticut. . Environ Entomol 9:, 668–672.[CrossRef]
    [Google Scholar]
  30. McClure M. S. , Andreadis T. G. , Lacy G. H. . ( 1982; ). Manipulating orchard ground cover to reduce invasion by leafhopper vectors of peach X-disease. . J Econ Entomol 75:, 64–68.[CrossRef]
    [Google Scholar]
  31. Nasu S. , Jensen D. D. , Richardson J. . ( 1970; ). Electron microscopy of Mycoplasma-like bodies associated with insect and plant hosts of peach Western X-disease. . Virology 41:, 583–595. [CrossRef] [PubMed]
    [Google Scholar]
  32. Nyland G. . ( 1971; ). Remission of symptoms of pear decline in pear and X-disease in peach after treatment with a tetracycline. . Phytopathology 61:, 904–905.
    [Google Scholar]
  33. Oshima K. , Kakizawa S. , Nishigawa H. , Jung H.-Y. , Wei W. , Suzuki S. , Arashida R. , Nakata D. , Miyata S. . & other authors ( 2004; ). Reductive evolution suggested from the complete genome sequence of a plant-pathogenic phytoplasma. . Nat Genet 36:, 27–29. [CrossRef] [PubMed]
    [Google Scholar]
  34. Pearson R. C. , Sands D. C. . ( 1978; ). Eastern X-disease of peach: Symptom remission and yield response following yearly injections of tetracyclines. . Plant Disease Reporter 62:, 753–757.
    [Google Scholar]
  35. Prince J. P. , Davis R. E. , Wolf T. K. , Lee I.-M. , Mogen B. D. , Dally E. L. , Bertaccini A. , Credi R. , Barba M. . ( 1993; ). Molecular detection of diverse mycoplasmalike organisms (MLOs) associated with grapevine yellows and their classification with aster yellows, X-disease, and elm yellows MLOs. . Phytopathology 83:, 1130–1137. [CrossRef]
    [Google Scholar]
  36. Rice R. E. , Jones R. A. . ( 1972; ). Leafhopper vectors of the western X-disease pathogen: collections in central California. . Environ Entomol 1:, 726–730.[CrossRef]
    [Google Scholar]
  37. Rosenberger D. A. , Jones A. L. . ( 1977; ). Symptom remission in X-diseased peach trees as affected by date, method, and rate of application of oxytetracycline-HCl. . Phytopathology 67:, 277–282. [CrossRef]
    [Google Scholar]
  38. Sands D. C. , Walton G. S. . ( 1975; ). Tetracycline injections for control of Eastern X-disease and bacterial spot of peach. . Plant Disease Reporter 59:, 573–576.
    [Google Scholar]
  39. Stoddard E. M. . ( 1934; ). Progress report on the investigation of a new peach disease. . Conn Pomol Soc Proc (1933) (44):, 31–36.
    [Google Scholar]
  40. Stoddard E. M. . ( 1938; ). The “X disease” of peach. . In June 1938 Circular, vol. 122, pp. 54–60. New Haven:: Connecticut Agricultural Experiment Station;.
    [Google Scholar]
  41. Stoddard E. M. , Hildebrand E. M. , Palmiter D. H. , Parker K. G. . ( 1951; ). X-disease. . In Virus Diseases and Other Disorders with Viruslike Symptoms of Stone Fruits in North America United States Department of Agriculture Agriculture Handbook, vol. 10, pp. 37–42. Washington:: United States Government Printing Office;.
    [Google Scholar]
  42. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  43. Wei W. , Davis R. E. , Lee I.-M. , Zhao Y. . ( 2007; ). Computer-simulated RFLP analysis of 16S rRNA genes: identification of ten new phytoplasma groups. . Int J Syst Evol Microbiol 57:, 1855–1867. [CrossRef] [PubMed]
    [Google Scholar]
  44. Zhao Y. , Wei W. , Lee I.-M. , Shao J. , Suo X. , Davis R. E. . ( 2009; ). Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). . Int J Syst Evol Microbiol 59:, 2582–2593. [CrossRef] [PubMed]
    [Google Scholar]
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