1887

Abstract

A novel nitrogen-fixing strain, designated HL-12, was isolated from a freshwater lake in Taiwan. Cells of strain HL-12 were aerobic, Gram-negative, motile rods that were surrounded by a thick capsule, contained poly-β-hydroxybutyrate granules, and formed light-yellow to brownish-red colonies. Growth occurred at 15–40 °C (optimum 25–35 °C), at pH 6.0–7.0 (optimum pH 6.0) and with 0–4 % NaCl (optimum 0–1 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HL-12 belonged to the genus and exhibited 99.1 and 98.8 % 16S rRNA gene sequence similarity, respectively, with IAM 14990 and IAM 13946. The major fatty acids (>10 %) of strain HL-12 were summed feature 3 (comprising Cω7 and/or Cω6), C and Cω7. The cellular hydroxy fatty acids were C 3-OH, C 2-OH and C 3-OH. The isoprenoid quinone was Q-8 and the DNA G+C content was 72.0 mol%. The polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several unknown aminophospholipids and phospholipids. DNA–DNA relatedness between strain HL-12 and LMG 3975 and LMG 3977 was <70 %. On the basis of the genotypic and phenotypic data, strain HL-12 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is HL-12 ( = BCRC 80208  = KCTC 23311).

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2013-03-01
2020-01-17
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