1887

Abstract

A novel obligately anaerobic, mesophilic, organotrophic bacterium, strain P3M-1, was isolated from a microbial mat formed in a wooden bath filled with hot water emerging from a 2775 m-deep well in the Tomsk region of western Siberia, Russia. Cells of strain P3M-1 were rod-shaped, 0.3–0.7 µm in width and formed multicellullar filaments that reached up to 400 µm in length. Strain P3M-1 grew optimally at 42–45 °C, pH 7.5–8.0, and with 0.1% (w/v) NaCl. Under optimal conditions, the doubling time was 6 h. The isolate was able to ferment a variety of proteinaceous substrates and sugars, including microcrystalline cellulose. Acetate, ethanol and H were the main products of glucose fermentation. The genomic DNA G+C content was 55 mol%. 16S rRNA gene sequence-based phylogenetic analyses showed that strain P3M-1 was a member of the class , with 92.8 % sequence similarity to KIBI-1. Based on phylogenetic analysis and physiological properties, strain P3M-1 represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed; the type strain of is P3M-1 ( = DSM 23815 = VKM B-2669).

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2013-01-01
2020-01-27
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