1887

Abstract

Two novel species of that are clearly distinct from all known species in section were revealed during a study of microfungal communities in Spanish caves. The novel species identified in this study and additional species of section are associated with places and substrates related to human activities in caves. Novel species are described using data from four loci (ITS, , and ), morphology and basic chemical and physiological analyses. Members of the species sp. nov. were isolated from various substrates, including decaying organic matter, cave air and cave sediment of the Cueva del Tesoro Cave (the Treasure cave); the species is represented by twelve isolates and is most closely related to the recently described . Members of the species sp. nov. were isolated from cave sediment in the Gruta de las Maravillas Cave (the Grotto of the Marvels); the species is represented by two isolates. An additional isolate was found in the Cueva del Tesoro Cave and in the Demänovská Peace Cave (Slovakia), suggesting a potentially wide distribution of this micro-organism. The species is related to and . Both species were unable to grow at 37 °C, and a weakly positive, light greenish yellow Ehrlich reaction was observed in . . Unique morphological features alone are sufficient to distinguish both species from related taxa.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.041004-0
2012-11-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/11/2778.html?itemId=/content/journal/ijsem/10.1099/ijs.0.041004-0&mimeType=html&fmt=ahah

References

  1. Adetutu E. M. , Thorpe K. , Bourne S. , Cao X. , Shahsavari E. , Kirby G. , Ball A. S. . ( 2011; ). Phylogenetic diversity of fungal communities in areas accessible and not accessible to tourists in Naracoorte Caves. . Mycologia 103:, 959–968. [CrossRef] [PubMed]
    [Google Scholar]
  2. Atlas R. M. . ( 2010; ). Handbook of Microbiological Media. Boca Raton:: CRC Press;. [CrossRef]
    [Google Scholar]
  3. Bastian F. , Alabouvette C. , Saiz-Jimenez C. . ( 2009; ). The impact of arthropods on fungal community structure in Lascaux Cave. . J Appl Microbiol 106:, 1456–1462. [CrossRef] [PubMed]
    [Google Scholar]
  4. Berrocal Pérez J. A. , Wallace Moreno L. . ( 1988; ). Guía de las cuevas de Málaga. Málaga:: Diputación Provincial de Málaga;.
    [Google Scholar]
  5. Buttner M. P. , Stetzenbach L. D. . ( 1991; ). Evaluation of four aerobiological sampling methods for the retrieval of aerosolized Pseudomonas syringae . . Appl Environ Microbiol 57:, 1268–1270.[PubMed]
    [Google Scholar]
  6. Fakih M. G. , Barden G. E. , Oakes C. A. , Berenson C. S. . ( 1995; ). First reported case of Aspergillus granulosus infection in a cardiac transplant patient. . J Clin Microbiol 33:, 471–473.[PubMed]
    [Google Scholar]
  7. Frisvad J. C. , Samson R. A. . ( 2004; ). Polyphasic taxonomy of Penicillium subgenus Penicillium: A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins. . Stud Mycol 49:, 1–173.
    [Google Scholar]
  8. Garrett S. D. . ( 1981; ). Soil Fungi and Soil Fertility: an Introduction to Soil Mycology. Oxford:: Pergamon Press;.
    [Google Scholar]
  9. Giménez Reyna S. , Laza Palacios M. . ( 1964; ). Informe de las excavaciones en la Cueva del Higuerón o del Suizo. Madrid:: Informe de las Excavaciones en la Cueva del Higuerón o Suizo;.
    [Google Scholar]
  10. Hall T. . ( 2004; ). BioEdit 7.0.0. Raleigh:: North Carolina State University;.
    [Google Scholar]
  11. Hermosín B. , Novakova A. , Jurado V. , Laiz Trobajo L. , Porca E. , Rogerio Candelera M. A. , Sánchez-Moral S. , Sáiz-Jiménez C. . ( 2010; ). Observatorio microbiológico de cuevas: evaluación y control de comunidades fúngicas en cuevas sometidas al impacto de actividades turísticas. . In Cuevas: Patrimonio, Naturaleza, Cultura y Turismo, pp. 513–520. Edited by Durán J. J. , Carrasco F. . . Madrid:: Asociación de Cuevas Turísticas Españolas;.
    [Google Scholar]
  12. Hong S. B. , Cho H. S. , Shin H. D. , Frisvad J. C. , Samson R. A. . ( 2006; ). Novel Neosartorya species isolated from soil in Korea. . Int J Syst Evol Microbiol 56:, 477–486. [CrossRef] [PubMed]
    [Google Scholar]
  13. Houbraken J. , Due M. , Varga J. , Meijer M. , Frisvad J. C. , Samson R. A. . ( 2007; ). Polyphasic taxonomy of Aspergillus section Usti . . Stud Mycol 59:, 107–128. [CrossRef] [PubMed]
    [Google Scholar]
  14. Jurado V. , Porca E. , Cuezva S. , Fernandez-Cortes A. , Sanchez-Moral S. , Saiz-Jimenez C. . ( 2010; ). Fungal outbreak in a show cave. . Sci Total Environ 408:, 3632–3638. [CrossRef] [PubMed]
    [Google Scholar]
  15. Katoh K. , Kuma K. , Toh H. , Miyata T. . ( 2005; ). MAFFT version 5: improvement in accuracy of multiple sequence alignment. . Nucleic Acids Res 33:, 511–518. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kelly K. L. . ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office. .
    [Google Scholar]
  17. Kelly K. L. . ( 1993; ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office. .
    [Google Scholar]
  18. Klich M. A. . ( 1993; ). Morphological studies of Aspergillus section Versicolores and related species. . Mycologia 85:, 100–107. [CrossRef]
    [Google Scholar]
  19. Kozakiewicz Z. . ( 1989; ). Aspergillus species on stored products. . Mycol Pap 161:, 1–188.
    [Google Scholar]
  20. Martín-Rosales W. , López-Chicano M. , Rodríguez C. M. , Pulido-Bosch A. . ( 1995; ). Morfología, espeleotemas y climatología de la Gruta de las Maravillas, Aracena (Huelva). . Espeleotemas 5:, 51–62.
    [Google Scholar]
  21. Northup D. E. , Lavoie K. H. . ( 2001; ). Geomicrobiology of caves: a review. . Geomicrobiol J 18:, 199–222. [CrossRef]
    [Google Scholar]
  22. Nováková A. . ( 2009; ). Microscopic fungi isolated from the Domica Cave system (Slovak Karst National Park, Slovakia). A review. . Int J Speleol 38:, 71–82.[CrossRef]
    [Google Scholar]
  23. Peterson S. W. . ( 2000; ). Phylogenetic relationships in Aspergillus based on rDNA sequence analysis. . In Integration of Modern Taxonomic Methods for Penicillium and Aspergillus Classification, pp. 323–356. Edited by Samson R. A. , Pitt J. I. . . Amsterdam:: Harwood Academic Publishers;.
    [Google Scholar]
  24. Peterson S. W. . ( 2008; ). Phylogenetic analysis of Aspergillus species using DNA sequences from four loci. . Mycologia 100:, 205–226. [CrossRef] [PubMed]
    [Google Scholar]
  25. Peterson S. W. , Varga J. , Frisvad J. C. , Samson R. A. . ( 2008; ). Phylogeny and subgeneric taxonomy of Aspergillus . . In Aspergillus in the Genomic Era, pp. 33–53. Edited by Varga J. , Samson R. A. . . Wageningen:: Wageningen Academic Publishers;.
    [Google Scholar]
  26. Pulido-Bosch A. , Martin-Rosales W. , López-Chicano M. , Rodriguez-Navarro C. M. , Vallejos A. . ( 1997; ). Human impact in a tourist karstic cave (Aracena, Spain). . Environ Geol 31:, 142–149. [CrossRef]
    [Google Scholar]
  27. Raper K. B. , Fennell D. I. . ( 1965; ). The genus Aspergillus. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  28. Robinson W. F. , Connole M. D. , King T. J. , Pitt J. I. , Moss S. M. . ( 2000; ). Systemic mycosis due to Aspergillus deflectus in a dog. . Aust Vet J 78:, 600–602. [CrossRef] [PubMed]
    [Google Scholar]
  29. Rutherford J. M. , Huang L. H. . ( 1994; ). A study of fungi of remote sediments in West Virginia caves and a comparison with reported species in the literature. . J Cave Karst Stud 56:, 38–45.
    [Google Scholar]
  30. Samson R. A. , Noonim P. , Meijer M. , Houbraken J. , Frisvad J. C. , Varga J. . ( 2007; ). Diagnostic tools to identify black aspergilli. . Stud Mycol 59:, 129–145. [CrossRef] [PubMed]
    [Google Scholar]
  31. Samson R. A. , Varga J. , Meijer M. , Frisvad J. C. . ( 2011; ). New taxa in Aspergillus section Usti . . Stud Mycol 69:, 81–97. [CrossRef] [PubMed]
    [Google Scholar]
  32. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  33. Varga J. , Houbraken J. , Van Der Lee H. A. L. , Verweij P. E. , Samson R. A. . ( 2008; ). Aspergillus calidoustus sp. nov., causative agent of human infections previously assigned to Aspergillus ustus . . Eukaryot Cell 7:, 630–638. [CrossRef] [PubMed]
    [Google Scholar]
  34. Vaughan M. J. , Maier R. M. , Pryor B. M. . ( 2011; ). Fungal communities on speleothem surfaces in Kartchner Caverns, Arizona, USA. . Int J Speleol 40:, 65–77. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.041004-0
Loading
/content/journal/ijsem/10.1099/ijs.0.041004-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error