1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_6

sp. nov., an extremely halophilic, fermentative, strictly anaerobic bacterium from sediments of the hypersaline lake Sehline Sebkha Free

Abstract

A strictly anaerobic, extremely halophilic, Gram-positive, rod-shaped bacterium was isolated from the hypersaline (>20 % NaCl) surface sediments of Sehline Sebkha in Tunisia. The strain, designated 1Sehel, was strictly halophilic and proliferated at NaCl concentrations of between 5 % and 30 % (saturation), with optimal growth at 20 % NaCl. Strain 1Sehel was non-spore-forming, non-motile, appearing singly or in pairs, or occasionally as long chains and measured 0.5–0.8 µm by 3–10 µm. Strain 1Sehel grew optimally at pH values of 7.4 but had a very broad pH range for growth (pH 5.2–9.4). It grew at temperatures between 20 and 50 °C with an optimum at 43 °C. Strain 1Sehel required yeast extract for growth. The isolate fermented glucose, galactose, fructose, glycerol, mannose, maltose, ribose, pyruvate and sucrose. The fermentation products from glucose utilization were lactate, acetate, formate, ethanol, CO and H. The G+C ratio of the DNA was 32.7 mol%. The major fatty acids were Cω6/7 Cω7, C and C On the basis of phylogenetic and physiological properties, strain 1Sehel ( = DSM 25582 = JCM 18213) is proposed as the type strain of sp. nov., within the family .

  • Published Online:
Funding
This study was supported by the:
  • Ministry for Scientific, Technology and Development
© 2013 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.040139-0
2013-06-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/6/2069.html?itemId=/content/journal/ijsem/10.1099/ijs.0.040139-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. ( 1979 ). Methanogens: reevaluation of a unique biological group. . Microbiol Rev 43, 260–296.[PubMed]
    [Google Scholar]
  3. Ben Dhia Thabet O. B., Fardeau M.-L., Joulian C., Thomas P., Hamdi M., Garcia J.-L., Ollivier B. ( 2004 ). Clostridium tunisiense sp. nov., a new proteolytic, sulfur-reducing bacterium isolated from an olive mill wastewater contaminated by phosphogypse. . Anaerobe 10, 185–190. [View Article] [PubMed]
    [Google Scholar]
  4. Bhupathiraju V. K., Oren A., Sharma P. K., Tanner R. S., Woese C. R., McInerney M. J. ( 1994 ). Haloanaerobium salsugo sp. nov., a moderately halophilic, anaerobic bacterium from a subterranean brine. . Int J Syst Bacteriol 44, 565–572. [View Article] [PubMed]
    [Google Scholar]
  5. Bhupathiraju V. K., McInerney M. J., Woese C. R., Tanner R. S. ( 1999 ). Haloanaerobium kushneri sp. nov., an obligately halophilic, anaerobic bacterium from an oil brine. . Int J Syst Bacteriol 49, 953–960. [View Article] [PubMed]
    [Google Scholar]
  6. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461–466. [View Article] [PubMed]
    [Google Scholar]
  7. Cayol J.-L., Ollivier B., Lawson Anani Soh A., Fardeau M.-L., Ageron E., Grimont P. A. D., Prensier G., Guezennec J. M., Magot M., Garcia J.-L. ( 1994 ). Haloincola saccharolytica subsp. senegalensis subsp. nov., isolated from the sediments of a hypersaline lake, and emended description of Haloincola saccharolytica . . Int J Syst Bacteriol 44, 805–811. [View Article]
    [Google Scholar]
  8. Cayol J.-L., Ollivier B., Patel B. K. C., Ageron E., Grimont P. A. D., Prensier G., Garcia J.-L. ( 1995 ). Haloanaerobium lacusroseus sp. nov., an extremely halophilic fermentative bacterium from the sediments of a hypersaline lake. . Int J Syst Bacteriol 45, 790–797. [View Article] [PubMed]
    [Google Scholar]
  9. Cole J. R., Wang Q., Cardenas E., Fish J., Chai B., Farris R. J., Kulam-Syed-Mohideen A. S., McGarrell D. M., Marsh T. et al. ( 2009 ). The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. . Nucleic Acids Res 37 (Database issue), D141–D145. [View Article] [PubMed]
    [Google Scholar]
  10. Cord-Ruwisch R. ( 1985 ). A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate reducing bacteria. . J Microbiol Methods 4, 33–36. [View Article]
    [Google Scholar]
  11. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [View Article] [PubMed]
    [Google Scholar]
  12. Fardeau M.-L., Ollivier B., Patel B. K. C., Magot M., Thomas P., Rimbault A., Rocchiccioli F., Garcia J.-L. ( 1997 ). Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. . Int J Syst Bacteriol 47, 1013–1019. [View Article] [PubMed]
    [Google Scholar]
  13. Fardeau M.-L., Magot M., Patel B. K. C., Thomas P., Garcia J.-L., Ollivier B. ( 2000 ). Thermoanaerobacter subterraneus sp. nov., a novel thermophile isolated from oilfield water. . Int J Syst Evol Microbiol 50, 2141–2149. [View Article] [PubMed]
    [Google Scholar]
  14. Felsenstein, J. (1993). phylip (phylogeny inference package) version 3.5.1c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  15. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 95–98.
     [Google Scholar]
  16. Hungate R. E. ( 1969 ). A roll tube method for cultivation of strict anaerobes. . Methods Microbiol 3B, 117–132. [View Article]
    [Google Scholar]
  17. Huss V. A., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184–192. [View Article] [PubMed]
    [Google Scholar]
  18. Imhoff J. F. ( 2005 ). Family II. Ectothiorhodospiraceae Imhoff part B, The Gammaproteobacteria. . In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 41–52. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;.
     [Google Scholar]
  19. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. R. . New York:: Academic Press;.
     [Google Scholar]
  20. Kobayashi T., Kimura B., Fujii T. ( 2000 ). Haloanaerobium fermentans sp. nov., a strictly anaerobic, fermentative halophile isolated from fermented puffer fish ovaries. . Int J Syst Evol Microbiol 50, 1621–1627. [View Article] [PubMed]
    [Google Scholar]
  21. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E. ( 1988 ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38, 358–361. [View Article]
    [Google Scholar]
  22. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  23. Miller L. T. ( 1982 ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16, 584–586.[PubMed]
    [Google Scholar]
  24. Miller T. L., Wolin M. J. ( 1974 ). A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. . Appl Microbiol 27, 985–987.[PubMed]
    [Google Scholar]
  25. Ollivier B., Caumette P., Garcia J.-L., Mah R. A. ( 1994 ). Anaerobic bacteria from hypersaline environments. . Microbiol Rev 58, 27–38.[PubMed]
    [Google Scholar]
  26. Oren A., Paster B. J., Woese C. R. ( 1984 ). Haloanaerobiaceae: a new family of moderately halophilic obligatory anaerobic bacteria. . Syst Appl Microbiol 5, 71–80. [View Article]
    [Google Scholar]
  27. Rainey F. A., Zhilina T. N., Boulygina E. S., Stackebrandt E., Tourova T. P., Zavarzin G. A. ( 1995 ). The taxonomic status of the fermentative halophilic anaerobic bacteria: description of Haloanaerobiales ord. nov., Halobacteroidaceae fam. nov., Orenia gen. nov. and further taxonomic rearrangements at the genus and species level. . Anaerobe 1, 185–199. [View Article] [PubMed]
    [Google Scholar]
  28. Ravot G., Magot M., Ollivier B., Patel B. K. C., Ageron E., Grimont P. A. D., Thomas P., Garcia J.-L. ( 1997 ). Haloanaerobium congolense sp. nov., an anaerobic, moderately halophilic, thiosulfate- and sulfur-reducing bacterium from an African oil field. . FEMS Microbiol Lett 147, 81–88. [View Article] [PubMed]
    [Google Scholar]
  29. Saitou N., Nei M. ( 1987 ). The neighbour-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 405–425.
     [Google Scholar]
  30. Tsai C.-R., Garcia J.-L., Patel B. K. C., Cayol J.-L., Baresi L., Mah R. A. ( 1995 ). Haloanaerobium alcaliphilum sp. nov., an anaerobic moderate halophile from the sediments of Great Salt Lake, Utah. . Int J Syst Bacteriol 45, 301–307. [View Article] [PubMed]
    [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  32. Winker S., Woese C. R. ( 1991 ). A definition of the domains Archaea, Bacteria and Eucarya in terms of small subunit ribosomal RNA characteristics. . Syst Appl Microbiol 14, 305–310. [View Article] [PubMed]
    [Google Scholar]
  33. Zeikus J. G., Hegge P. W., Thompson T. E., Phelps T. J., Langworthy T. A. ( 1983 ). Isolation and description of Haloanaerobium praevalens gen. nov. and sp. nov., an obligately anaerobic halophile common to Great Salt Lake sediments. . Curr Microbiol 9, 225–233. [View Article]
    [Google Scholar]
  34. Zhilina T. N., Zavarzin G. A., Bulygina E. S., Kevbrin V. V., Osipov G. A., Chumakov K. M. ( 1992 ). Ecology, physiology and taxonomy studies on a new taxon of Haloanaerobiaceae, Haloincola saccharolytica, gen. nov., sp. nov.. Syst Appl Microbiol 15, 275–284. [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.040139-0
Loading
Halanaerobium sehlinense sp. nov., an extremely halophilic, fermentative, strictly anaerobic bacterium from sediments of the hypersaline lake Sehline Sebkha
Int J Syst Evol Microbiol 63, 2069 (2013); https://doi.org/10.1099/ijs.0.040139-0
/content/journal/ijsem/10.1099/ijs.0.040139-0
/content/journal/ijsem/10.1099/ijs.0.040139-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error