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Volume 62, Issue Pt_11

gen. nov., sp. nov., an obligately anaerobic, alkaliphilic, xylanolytic bacterium from a meromictic soda lake Free

Abstract

A Gram-positive, obligately anaerobic, motile, slender, flexible rod, designated SC/BZ-SP2, was isolated from mixed alkaline water and sediment of Soap Lake, Washington State, USA. Strain SC/BZ-SP2 formed salmon to pink colonies and was alkaliphilic. The isolate grew at pH 7.5–10.5 (optimum pH 9.7), at 8–40 °C (optimum 35–37 °C) and with 0.35–1.38 M Na (optimum 0.44–0.69 M Na). The isolate utilized -arabinose, -ribose, -xylose, -fructose, -mannose, -galactose, cellobiose, maltose, sucrose, trehalose, sorbitol, xylan, malate and yeast extract as carbon and energy sources; best growth was observed with -arabinose, cellobiose, maltose and trehalose. The major fermentation products from beechwood xylan were propionate and acetate. The dominant fatty acids were iso-C, anteiso-C, iso-C 3-OH, C 3-OH and C 3-OH. The cell-wall sugars were ribose, xylose, galactose and glucose. Thiosulfate and sulfite could be reduced to sulfide. The genomic DNA G+C content was 39.5±0.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SC/BZ-SP2 belonged to the family of the order , class The most closely related strains were Z-7010 (91.8 % 16S rRNA gene sequence similarity), Cy s1 (91.0 %) and Fru22 (90.4 %). On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain SC/BZ-SP2 represents a novel species in a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is SC/BZ-SP2 ( = ATCC BAA-2172  = DSM 24412).

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2012-11-01
2024-04-26
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Alkalitalea saponilacus gen. nov., sp. nov., an obligately anaerobic, alkaliphilic, xylanolytic bacterium from a meromictic soda lake
Int J Syst Evol Microbiol 62, 2618 (2012); https://doi.org/10.1099/ijs.0.038315-0
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